Characterization of oil palm fronds hemicelluloses extracted by subcritical water extraction / Norsyabilah Ridzuan

Oil palm plantations in Malaysia generate more than 36 million tonnes of pruned and felled oil palm fronds (OPF) and are generally considered as wastes. Fractionation of hemicelluloses from OPF using a hydrothermal process could be of interest in obtaining useable substrate for different product ap...

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Main Author:	Ridzuan, Norsyabilah
Format:	Thesis
Language:	English
Published:	2015
Subjects:	Extraction (Chemistry) Palm oil
Online Access:	http://ir.uitm.edu.my/id/eprint/14224/ http://ir.uitm.edu.my/id/eprint/14224/1/TM_NORSYABILAH%20RIDZUAN%20AS%2015_5.pdf

id	uitm-14224
recordtype	eprints
spelling	uitm-142242016-07-28T08:58:58Z http://ir.uitm.edu.my/id/eprint/14224/ Characterization of oil palm fronds hemicelluloses extracted by subcritical water extraction / Norsyabilah Ridzuan Ridzuan, Norsyabilah Extraction (Chemistry) Palm oil Oil palm plantations in Malaysia generate more than 36 million tonnes of pruned and felled oil palm fronds (OPF) and are generally considered as wastes. Fractionation of hemicelluloses from OPF using a hydrothermal process could be of interest in obtaining useable substrate for different product applications. The aim of this study was to optimize the subcritical water extraction (SWE) conditions of oil palm fronds on the hemicelluloses yield and consequently on the production of high molecular weight hemicelluloses (HMH) and low molecular weight hemicelluloses (LMH). In order to optimize the hemicelluloses yield, the effects of temperature (180-200°C), pressure (500-700 psi) and time (5-15 min) of SWE were examined applying three level Box Behnken design and response surface methodology (RSM). The liquid extract obtained at optimized conditions of subcritical water extraction was then precipitated with ethanol and filtered to produce high molecular weight hemicelluloses (solid precipitate). The supernatant was further separated using ultrafiltration lOkDa molecular weight cut off membrane to obtain low molecular weight hemicelluloses. All the samples were characterized for monosaccharides, xylooligosaccharides, lignin content, FTIR spectra and molecular weight determination. The statistical analysis indicated that three variables of SWE conditions had significant effects on the yields of hemicelluloses (p<0.05). The considered optimum conditions were: temperature of 185°C, pressure of 650 psi and contact time of 7 mins was 86.22%. Under these optimal conditions 16.64 mg/ml monosaccharides concentration of liquid extract, 7.86 mg/ml of HMH, 13. 72mg/ml ret en tate of LMH and 13.1 7mg/ml permeate of LMH were obtained. The monosaccharides composition of the liquid extract, HMH and LMH (retentate and permeate) contained xylose as major constituent followed by arabinose. This study demonstrated that the maximum yield of oil palm fronds hemicelluloses could be achieved by optimized the conditions of SWE through the RSM. The HMH and LMH separated via ultrafilltration membrane would be further investigated in the pharmaceutical and food applications 2015 Thesis NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/14224/1/TM_NORSYABILAH%20RIDZUAN%20AS%2015_5.pdf Ridzuan, Norsyabilah (2015) Characterization of oil palm fronds hemicelluloses extracted by subcritical water extraction / Norsyabilah Ridzuan. Masters thesis, Universiti Teknologi MARA.
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Teknologi MARA
building	UiTM Institutional Repository
collection	Online Access
language	English
topic	Extraction (Chemistry) Palm oil
spellingShingle	Extraction (Chemistry) Palm oil Ridzuan, Norsyabilah Characterization of oil palm fronds hemicelluloses extracted by subcritical water extraction / Norsyabilah Ridzuan
description	Oil palm plantations in Malaysia generate more than 36 million tonnes of pruned and felled oil palm fronds (OPF) and are generally considered as wastes. Fractionation of hemicelluloses from OPF using a hydrothermal process could be of interest in obtaining useable substrate for different product applications. The aim of this study was to optimize the subcritical water extraction (SWE) conditions of oil palm fronds on the hemicelluloses yield and consequently on the production of high molecular weight hemicelluloses (HMH) and low molecular weight hemicelluloses (LMH). In order to optimize the hemicelluloses yield, the effects of temperature (180-200°C), pressure (500-700 psi) and time (5-15 min) of SWE were examined applying three level Box Behnken design and response surface methodology (RSM). The liquid extract obtained at optimized conditions of subcritical water extraction was then precipitated with ethanol and filtered to produce high molecular weight hemicelluloses (solid precipitate). The supernatant was further separated using ultrafiltration lOkDa molecular weight cut off membrane to obtain low molecular weight hemicelluloses. All the samples were characterized for monosaccharides, xylooligosaccharides, lignin content, FTIR spectra and molecular weight determination. The statistical analysis indicated that three variables of SWE conditions had significant effects on the yields of hemicelluloses (p<0.05). The considered optimum conditions were: temperature of 185°C, pressure of 650 psi and contact time of 7 mins was 86.22%. Under these optimal conditions 16.64 mg/ml monosaccharides concentration of liquid extract, 7.86 mg/ml of HMH, 13. 72mg/ml ret en tate of LMH and 13.1 7mg/ml permeate of LMH were obtained. The monosaccharides composition of the liquid extract, HMH and LMH (retentate and permeate) contained xylose as major constituent followed by arabinose. This study demonstrated that the maximum yield of oil palm fronds hemicelluloses could be achieved by optimized the conditions of SWE through the RSM. The HMH and LMH separated via ultrafilltration membrane would be further investigated in the pharmaceutical and food applications
format	Thesis
author	Ridzuan, Norsyabilah
author_facet	Ridzuan, Norsyabilah
author_sort	Ridzuan, Norsyabilah
title	Characterization of oil palm fronds hemicelluloses extracted by subcritical water extraction / Norsyabilah Ridzuan
title_short	Characterization of oil palm fronds hemicelluloses extracted by subcritical water extraction / Norsyabilah Ridzuan
title_full	Characterization of oil palm fronds hemicelluloses extracted by subcritical water extraction / Norsyabilah Ridzuan
title_fullStr	Characterization of oil palm fronds hemicelluloses extracted by subcritical water extraction / Norsyabilah Ridzuan
title_full_unstemmed	Characterization of oil palm fronds hemicelluloses extracted by subcritical water extraction / Norsyabilah Ridzuan
title_sort	characterization of oil palm fronds hemicelluloses extracted by subcritical water extraction / norsyabilah ridzuan
publishDate	2015
url	http://ir.uitm.edu.my/id/eprint/14224/ http://ir.uitm.edu.my/id/eprint/14224/1/TM_NORSYABILAH%20RIDZUAN%20AS%2015_5.pdf
first_indexed	2023-09-18T22:51:09Z
last_indexed	2023-09-18T22:51:09Z
_version_	1777417552711909376

Characterization of oil palm fronds hemicelluloses extracted by subcritical water extraction / Norsyabilah Ridzuan

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