Extraction and partial purification of protease from Bilimbi (Averrhoa bilimbi L.) / Normah Ismail and Nur' Ain Mohamad Kharoe

Unripe and ripe bilimbi (Averrhoa bilimbi L.) were ground and the extracted juices were partially purified by ammonium sulfate precipitation at the concentrations of40 and 60% (w/v). The collected proteases were analysed for pH, temperature stability, storage stability, molecular weight distributio...

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Main Authors:	Ismail, Normah, Mohamad Kharoe, Nur' Ain
Format:	Article
Language:	English
Published:	Research Management Institute (RMI) 2013
Subjects:	Oxalidaceae (Wood sorrel) Proteolytic enzymes. Proteases Ammonium sulfate
Online Access:	http://ir.uitm.edu.my/id/eprint/13046/ http://ir.uitm.edu.my/id/eprint/13046/1/AJ_NORMAH%20ISMAIL%20%282%29%20SRJ%2013.pdf

id	uitm-13046
recordtype	eprints
spelling	uitm-130462016-05-30T04:30:54Z http://ir.uitm.edu.my/id/eprint/13046/ Extraction and partial purification of protease from Bilimbi (Averrhoa bilimbi L.) / Normah Ismail and Nur' Ain Mohamad Kharoe Ismail, Normah Mohamad Kharoe, Nur' Ain Oxalidaceae (Wood sorrel) Proteolytic enzymes. Proteases Ammonium sulfate Unripe and ripe bilimbi (Averrhoa bilimbi L.) were ground and the extracted juices were partially purified by ammonium sulfate precipitation at the concentrations of40 and 60% (w/v). The collected proteases were analysed for pH, temperature stability, storage stability, molecular weight distribution, protein concentration and protein content. Protein content of bilimbi fruit was 0.89 g. Protease activity ofboth the unripe and ripe fruit were optimum at pH 4 and 40°C when the juice were purified at 40 and 60% ammonium sulfate precipitation. A decreased in protease activity was observed during the seven days of storage at 4°C. Molecular weight distribution indicated that the proteases protein bands fall between 10 to 220 kDa. Protein bands were observed at 25, 50 and 160 kDa in both the unripe and ripe bilimbi proteases purified with 40% ammonium sulfate, however, the bands were more intense in those from unripe bilimbi. No protein bands were seen in proteases purified with 60% ammonium sulfate. Protein concentration was higher for proteases extracted with 40% ammonium sulfate at both ripening stages. Thus, purification using 40% ammonium sulfate precipitation could be a successful method to partially purify proteases from bilimbi especially from the unripe stage. Research Management Institute (RMI) 2013 Article PeerReviewed text en http://ir.uitm.edu.my/id/eprint/13046/1/AJ_NORMAH%20ISMAIL%20%282%29%20SRJ%2013.pdf Ismail, Normah and Mohamad Kharoe, Nur' Ain (2013) Extraction and partial purification of protease from Bilimbi (Averrhoa bilimbi L.) / Normah Ismail and Nur' Ain Mohamad Kharoe. Scientific Research Journal, 10 (2). pp. 29-49. ISSN 1675-7009
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Teknologi MARA
building	UiTM Institutional Repository
collection	Online Access
language	English
topic	Oxalidaceae (Wood sorrel) Proteolytic enzymes. Proteases Ammonium sulfate
spellingShingle	Oxalidaceae (Wood sorrel) Proteolytic enzymes. Proteases Ammonium sulfate Ismail, Normah Mohamad Kharoe, Nur' Ain Extraction and partial purification of protease from Bilimbi (Averrhoa bilimbi L.) / Normah Ismail and Nur' Ain Mohamad Kharoe
description	Unripe and ripe bilimbi (Averrhoa bilimbi L.) were ground and the extracted juices were partially purified by ammonium sulfate precipitation at the concentrations of40 and 60% (w/v). The collected proteases were analysed for pH, temperature stability, storage stability, molecular weight distribution, protein concentration and protein content. Protein content of bilimbi fruit was 0.89 g. Protease activity ofboth the unripe and ripe fruit were optimum at pH 4 and 40°C when the juice were purified at 40 and 60% ammonium sulfate precipitation. A decreased in protease activity was observed during the seven days of storage at 4°C. Molecular weight distribution indicated that the proteases protein bands fall between 10 to 220 kDa. Protein bands were observed at 25, 50 and 160 kDa in both the unripe and ripe bilimbi proteases purified with 40% ammonium sulfate, however, the bands were more intense in those from unripe bilimbi. No protein bands were seen in proteases purified with 60% ammonium sulfate. Protein concentration was higher for proteases extracted with 40% ammonium sulfate at both ripening stages. Thus, purification using 40% ammonium sulfate precipitation could be a successful method to partially purify proteases from bilimbi especially from the unripe stage.
format	Article
author	Ismail, Normah Mohamad Kharoe, Nur' Ain
author_facet	Ismail, Normah Mohamad Kharoe, Nur' Ain
author_sort	Ismail, Normah
title	Extraction and partial purification of protease from Bilimbi (Averrhoa bilimbi L.) / Normah Ismail and Nur' Ain Mohamad Kharoe
title_short	Extraction and partial purification of protease from Bilimbi (Averrhoa bilimbi L.) / Normah Ismail and Nur' Ain Mohamad Kharoe
title_full	Extraction and partial purification of protease from Bilimbi (Averrhoa bilimbi L.) / Normah Ismail and Nur' Ain Mohamad Kharoe
title_fullStr	Extraction and partial purification of protease from Bilimbi (Averrhoa bilimbi L.) / Normah Ismail and Nur' Ain Mohamad Kharoe
title_full_unstemmed	Extraction and partial purification of protease from Bilimbi (Averrhoa bilimbi L.) / Normah Ismail and Nur' Ain Mohamad Kharoe
title_sort	extraction and partial purification of protease from bilimbi (averrhoa bilimbi l.) / normah ismail and nur' ain mohamad kharoe
publisher	Research Management Institute (RMI)
publishDate	2013
url	http://ir.uitm.edu.my/id/eprint/13046/ http://ir.uitm.edu.my/id/eprint/13046/1/AJ_NORMAH%20ISMAIL%20%282%29%20SRJ%2013.pdf
first_indexed	2023-09-18T22:49:44Z
last_indexed	2023-09-18T22:49:44Z
_version_	1777417464009719808

Extraction and partial purification of protease from Bilimbi (Averrhoa bilimbi L.) / Normah Ismail and Nur' Ain Mohamad Kharoe

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