Characterization and purification of protease extracted from two maturity stages of ‘Noni’ (Morinda citrifolia L.) fruit / Normah Ismail and Nurulain Abd Razak

Protease was extracted from two maturity stages of noni fruits (Morinda citrifolia L.), unripe (stage 1) and ripe (stage 5). The crude extract was partially purified by acetone precipitation method followed by dialysis, gel filtration chromatography and freeze drying. Protein concentrations, proteo...

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Main Authors:	Ismail, Normah, Abd Razak, Nurulain
Format:	Article
Language:	English
Published:	Research Management Institute (RMI) 2014
Subjects:	Rubiaceae (Madder). Morinda citrifolia Proteolytic enzymes. Proteases
Online Access:	http://ir.uitm.edu.my/id/eprint/12429/ http://ir.uitm.edu.my/id/eprint/12429/1/AJ_NORMAH%20ISMAIL%20SRJ%2014%201.pdf

id	uitm-12429
recordtype	eprints
spelling	uitm-124292016-05-26T02:39:20Z http://ir.uitm.edu.my/id/eprint/12429/ Characterization and purification of protease extracted from two maturity stages of ‘Noni’ (Morinda citrifolia L.) fruit / Normah Ismail and Nurulain Abd Razak Ismail, Normah Abd Razak, Nurulain Rubiaceae (Madder). Morinda citrifolia Proteolytic enzymes. Proteases Protease was extracted from two maturity stages of noni fruits (Morinda citrifolia L.), unripe (stage 1) and ripe (stage 5). The crude extract was partially purified by acetone precipitation method followed by dialysis, gel filtration chromatography and freeze drying. Protein concentrations, proteolytic activity, molecular weight distribution, pH stability, temperature stability and storage efficiency of the resulting protease were evaluated. The unripe and ripe noni fruit contains 0.65 and 0.35% protein, respectively. Molecular weight of the proteases from both stages ranged approximately between 3 to 28 kDa based on the SDS-PAGE results. The optimum activity were at pH 7s and 6, temperatures of 40 and 50°C, respectively for proteases obtained from the unripe and ripe fruit. Analysis from the freeze dried protease indicated that protease from ripe noni fruits had higher protein concentration and specific activity compared to those from unripe fruit. However, it is more sensitive to pH and temperature and less stable during storage as it shows lower proteolytic activity compared to protease from unripe fruit. Based on its high proteolytic activity reaching up to 70.31 U/mg and storage stability (30% lost of activity), noni fruit could be an alternative source of plant protease. Research Management Institute (RMI) 2014 Article PeerReviewed text en http://ir.uitm.edu.my/id/eprint/12429/1/AJ_NORMAH%20ISMAIL%20SRJ%2014%201.pdf Ismail, Normah and Abd Razak, Nurulain (2014) Characterization and purification of protease extracted from two maturity stages of ‘Noni’ (Morinda citrifolia L.) fruit / Normah Ismail and Nurulain Abd Razak. Scientific Research Journal, 11 (2). pp. 1-16. ISSN 1675-7009
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Teknologi MARA
building	UiTM Institutional Repository
collection	Online Access
language	English
topic	Rubiaceae (Madder). Morinda citrifolia Proteolytic enzymes. Proteases
spellingShingle	Rubiaceae (Madder). Morinda citrifolia Proteolytic enzymes. Proteases Ismail, Normah Abd Razak, Nurulain Characterization and purification of protease extracted from two maturity stages of ‘Noni’ (Morinda citrifolia L.) fruit / Normah Ismail and Nurulain Abd Razak
description	Protease was extracted from two maturity stages of noni fruits (Morinda citrifolia L.), unripe (stage 1) and ripe (stage 5). The crude extract was partially purified by acetone precipitation method followed by dialysis, gel filtration chromatography and freeze drying. Protein concentrations, proteolytic activity, molecular weight distribution, pH stability, temperature stability and storage efficiency of the resulting protease were evaluated. The unripe and ripe noni fruit contains 0.65 and 0.35% protein, respectively. Molecular weight of the proteases from both stages ranged approximately between 3 to 28 kDa based on the SDS-PAGE results. The optimum activity were at pH 7s and 6, temperatures of 40 and 50°C, respectively for proteases obtained from the unripe and ripe fruit. Analysis from the freeze dried protease indicated that protease from ripe noni fruits had higher protein concentration and specific activity compared to those from unripe fruit. However, it is more sensitive to pH and temperature and less stable during storage as it shows lower proteolytic activity compared to protease from unripe fruit. Based on its high proteolytic activity reaching up to 70.31 U/mg and storage stability (30% lost of activity), noni fruit could be an alternative source of plant protease.
format	Article
author	Ismail, Normah Abd Razak, Nurulain
author_facet	Ismail, Normah Abd Razak, Nurulain
author_sort	Ismail, Normah
title	Characterization and purification of protease extracted from two maturity stages of ‘Noni’ (Morinda citrifolia L.) fruit / Normah Ismail and Nurulain Abd Razak
title_short	Characterization and purification of protease extracted from two maturity stages of ‘Noni’ (Morinda citrifolia L.) fruit / Normah Ismail and Nurulain Abd Razak
title_full	Characterization and purification of protease extracted from two maturity stages of ‘Noni’ (Morinda citrifolia L.) fruit / Normah Ismail and Nurulain Abd Razak
title_fullStr	Characterization and purification of protease extracted from two maturity stages of ‘Noni’ (Morinda citrifolia L.) fruit / Normah Ismail and Nurulain Abd Razak
title_full_unstemmed	Characterization and purification of protease extracted from two maturity stages of ‘Noni’ (Morinda citrifolia L.) fruit / Normah Ismail and Nurulain Abd Razak
title_sort	characterization and purification of protease extracted from two maturity stages of ‘noni’ (morinda citrifolia l.) fruit / normah ismail and nurulain abd razak
publisher	Research Management Institute (RMI)
publishDate	2014
url	http://ir.uitm.edu.my/id/eprint/12429/ http://ir.uitm.edu.my/id/eprint/12429/1/AJ_NORMAH%20ISMAIL%20SRJ%2014%201.pdf
first_indexed	2023-09-18T22:49:15Z
last_indexed	2023-09-18T22:49:15Z
_version_	1777417433651347456

Characterization and purification of protease extracted from two maturity stages of ‘Noni’ (Morinda citrifolia L.) fruit / Normah Ismail and Nurulain Abd Razak

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