Enhancement of l-phenylalanine production by aminoacylase-chitosan complex / Pat M. Lee and Kong-Hung Lee
Aminoacylase (E.C.3.5.1.14) catalyses the enantioselective hydrolysis of N-acetyl-L-amino acids. It is immobilized by forming an insoluble complex with chitosan to produce Lphenylalanine from a racemic mixture of N-acetyl-DL-phenylalanine. The aminoacylasechitosan complex enhanced the yield of produ...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Faculty of Applied Sciences
2004
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| Subjects: | |
| Online Access: | http://ir.uitm.edu.my/id/eprint/11811/ http://ir.uitm.edu.my/id/eprint/11811/1/AJ_PAT%20M.%20LEE%20SL%2004.pdf |
| Summary: | Aminoacylase (E.C.3.5.1.14) catalyses the enantioselective hydrolysis of N-acetyl-L-amino acids. It is immobilized by forming an insoluble complex with chitosan to produce Lphenylalanine from a racemic mixture of N-acetyl-DL-phenylalanine. The aminoacylasechitosan complex enhanced the yield of production of L-phenylalanine by about 400% at
neutral pH, at 65 °c and at alkaline pH (7.5-8.5) at 37°c . |
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