Influence of fresh and thermoxidized carotino oil on Cyclic Guanosine Monophosphate (cGMP) in erythrocytes from Sprague dawley rats / Mohd Fakharul Zaman Raja Yahya and Athifah Najwani Shahidan
Cyclic guanosine monophosphate (cGMP) is a second messenger molecule involved in the intracellular signalling mechanism which is important in a wide range of cellular process including metabolism, gene expression, cell proliferation and cell death. This study was conducted to determine the effect of...
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Research Management Institute
2012
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| Online Access: | http://ir.uitm.edu.my/id/eprint/11107/ http://ir.uitm.edu.my/id/eprint/11107/1/AJ_MOHD%20FAKHARUL%20ZAMAN%20RAJA%20YAHYA%20%282%29%20SRJ%2012%201.pdf |
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uitm-111072016-05-16T07:42:12Z http://ir.uitm.edu.my/id/eprint/11107/ Influence of fresh and thermoxidized carotino oil on Cyclic Guanosine Monophosphate (cGMP) in erythrocytes from Sprague dawley rats / Mohd Fakharul Zaman Raja Yahya and Athifah Najwani Shahidan Raja Yahya, Mohd Fakharul Zaman Shahidan, Athifah Najwani Thermochemistry Cellular signal transduction. Including second messengers Cyclic guanylic acid Carotenes. Carotin Cyclic guanosine monophosphate (cGMP) is a second messenger molecule involved in the intracellular signalling mechanism which is important in a wide range of cellular process including metabolism, gene expression, cell proliferation and cell death. This study was conducted to determine the effect of fresh (FCO) and thermoxidized carotino oil (TCO) on erythrocyte cGMP levels from Sprague dawley rats. A total of 30 Sprague dawley rats were randomly segregated into three groups: the first of which was placed on a Fresh Carotino Oil (FCO) diet, the second on a Thermoxidized Carotino Oil (TCO) diet and the control group on commercial rat chow only for a period of 6 and 9 weeks. The two oil diets comprised of 20% (w:w) of each oil mixed with commercial rat feed. The enzyme immunoassays, performed in week 6, revealed that the erythrocytes cGMP levels for the FCO and TCO groups were 66.198±3.193 pmol/mL and 61.990±6.318 pmol/mL respectively, and were significantly (p<0.05) lower than the value for the control group, 77.978±10.479 pmol/mL. The assays performed in week 9 revealed the erythrocytes cGMP levels for the FCO and TCO groups to be 66.522±8.194 pmol/mL and 56.842±8.546 pmol/mL respectively which were also significantly (p<0.05) lower than that for the control group, 82.817±6.677 pmol/mL. The results indicate that the presence of antioxidants, such as beta-carotene and tocols in carotino oil may modulate cGMP levels in rats. Research Management Institute 2012 Article PeerReviewed text en http://ir.uitm.edu.my/id/eprint/11107/1/AJ_MOHD%20FAKHARUL%20ZAMAN%20RAJA%20YAHYA%20%282%29%20SRJ%2012%201.pdf Raja Yahya, Mohd Fakharul Zaman and Shahidan, Athifah Najwani (2012) Influence of fresh and thermoxidized carotino oil on Cyclic Guanosine Monophosphate (cGMP) in erythrocytes from Sprague dawley rats / Mohd Fakharul Zaman Raja Yahya and Athifah Najwani Shahidan. Scientific Research Journal, 9 (1). pp. 63-70. ISSN 1675-7009 |
| repository_type |
Digital Repository |
| institution_category |
Local University |
| institution |
Universiti Teknologi MARA |
| building |
UiTM Institutional Repository |
| collection |
Online Access |
| language |
English |
| topic |
Thermochemistry Cellular signal transduction. Including second messengers Cyclic guanylic acid Carotenes. Carotin |
| spellingShingle |
Thermochemistry Cellular signal transduction. Including second messengers Cyclic guanylic acid Carotenes. Carotin Raja Yahya, Mohd Fakharul Zaman Shahidan, Athifah Najwani Influence of fresh and thermoxidized carotino oil on Cyclic Guanosine Monophosphate (cGMP) in erythrocytes from Sprague dawley rats / Mohd Fakharul Zaman Raja Yahya and Athifah Najwani Shahidan |
| description |
Cyclic guanosine monophosphate (cGMP) is a second messenger molecule involved in the intracellular signalling mechanism which is important in a wide range of cellular process including metabolism, gene expression, cell proliferation and cell death. This study was conducted to determine the effect of fresh (FCO) and thermoxidized carotino oil (TCO) on erythrocyte cGMP levels from Sprague dawley rats. A total of 30 Sprague dawley rats were randomly segregated into three groups: the first of which was placed on a Fresh Carotino Oil (FCO) diet, the second on a Thermoxidized
Carotino Oil (TCO) diet and the control group on commercial rat chow only for a period of 6 and 9 weeks. The two oil diets comprised of 20% (w:w) of each oil mixed with commercial rat feed. The enzyme immunoassays, performed in week 6, revealed that the erythrocytes cGMP levels for the FCO and TCO groups were 66.198±3.193 pmol/mL and 61.990±6.318 pmol/mL respectively, and were significantly (p<0.05) lower than the value for the control group, 77.978±10.479 pmol/mL. The assays performed in week 9 revealed the erythrocytes cGMP levels for the FCO and TCO groups to be 66.522±8.194 pmol/mL and 56.842±8.546 pmol/mL respectively which were also significantly (p<0.05) lower than that for the control group, 82.817±6.677 pmol/mL. The results indicate that the presence of antioxidants, such as beta-carotene and tocols in carotino oil may modulate cGMP levels in rats. |
| format |
Article |
| author |
Raja Yahya, Mohd Fakharul Zaman Shahidan, Athifah Najwani |
| author_facet |
Raja Yahya, Mohd Fakharul Zaman Shahidan, Athifah Najwani |
| author_sort |
Raja Yahya, Mohd Fakharul Zaman |
| title |
Influence of fresh and thermoxidized carotino oil on Cyclic Guanosine Monophosphate (cGMP) in erythrocytes from Sprague dawley rats / Mohd Fakharul Zaman Raja Yahya and Athifah Najwani Shahidan |
| title_short |
Influence of fresh and thermoxidized carotino oil on Cyclic Guanosine Monophosphate (cGMP) in erythrocytes from Sprague dawley rats / Mohd Fakharul Zaman Raja Yahya and Athifah Najwani Shahidan |
| title_full |
Influence of fresh and thermoxidized carotino oil on Cyclic Guanosine Monophosphate (cGMP) in erythrocytes from Sprague dawley rats / Mohd Fakharul Zaman Raja Yahya and Athifah Najwani Shahidan |
| title_fullStr |
Influence of fresh and thermoxidized carotino oil on Cyclic Guanosine Monophosphate (cGMP) in erythrocytes from Sprague dawley rats / Mohd Fakharul Zaman Raja Yahya and Athifah Najwani Shahidan |
| title_full_unstemmed |
Influence of fresh and thermoxidized carotino oil on Cyclic Guanosine Monophosphate (cGMP) in erythrocytes from Sprague dawley rats / Mohd Fakharul Zaman Raja Yahya and Athifah Najwani Shahidan |
| title_sort |
influence of fresh and thermoxidized carotino oil on cyclic guanosine monophosphate (cgmp) in erythrocytes from sprague dawley rats / mohd fakharul zaman raja yahya and athifah najwani shahidan |
| publisher |
Research Management Institute |
| publishDate |
2012 |
| url |
http://ir.uitm.edu.my/id/eprint/11107/ http://ir.uitm.edu.my/id/eprint/11107/1/AJ_MOHD%20FAKHARUL%20ZAMAN%20RAJA%20YAHYA%20%282%29%20SRJ%2012%201.pdf |
| first_indexed |
2023-09-18T22:47:51Z |
| last_indexed |
2023-09-18T22:47:51Z |
| _version_ |
1777417345459814400 |