The effects of palm tocotrienol rich fraction (TRF) on proatherosclerotic changes in aorta of rats fed high methionine diet

The effects of palm tocotrienol rich fraction (TRF) on proatherosclerotic changes in aorta of rats fed high methionine diet

Hyperhomocysteinemia is a risk factor for cardiovascular diseases. It is associated with an increase in oxidative stress. We aimed to study the effects of palm tocotrienol rich fraction (palm TRF) on proatherosclerotic changes in aorta of rats fed high methionine diet. Methodology:42 male Wistar rat...

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Main Authors: Ku Zaifah, Norsidah, Yusuf, Kamisah, Yusuf, Ahmad Asmadi, Ayob, Azizi
Format: Conference or Workshop Item
Language:English
Published: 2009
Subjects:
RM214 Diet therapy. Diet and dietectics in disease
Online Access:http://irep.iium.edu.my/9922/
http://irep.iium.edu.my/9922/1/australia_2009_poster.pdf
id iium-9922
recordtype eprints
spelling iium-99222015-04-07T03:36:13Z http://irep.iium.edu.my/9922/ The effects of palm tocotrienol rich fraction (TRF) on proatherosclerotic changes in aorta of rats fed high methionine diet Ku Zaifah, Norsidah Yusuf, Kamisah Yusuf, Ahmad Asmadi Ayob, Azizi RM214 Diet therapy. Diet and dietectics in disease Hyperhomocysteinemia is a risk factor for cardiovascular diseases. It is associated with an increase in oxidative stress. We aimed to study the effects of palm tocotrienol rich fraction (palm TRF) on proatherosclerotic changes in aorta of rats fed high methionine diet. Methodology:42 male Wistar rats were divided into six groups. The first group was the control. Group 2 to 6 were fed 1% methionine diet for 10 weeks. From week 6 onward, folate (8 mg/kg diet) or palm TRF (30, 60 or 150 mg/kg diet) were added into the diet of group 3 (M+Folate), 4 (M+TRF30), 5 (M+TRF60) and 6 (M+TRF150) respectively. Body weights were recorded weekly. Statistical analysis:Statistical Package for Social Sciences (SPSS) 11.5 was used. Data is presented as Mean ± SEM. P < 0.05 is considered significant. No significant difference in body weight was recorded. Methionine diet (M) significantly raised the total plasma homocysteine (tHcy) level and this was reduced by the supplementations of palm TRF at 150 mg/kg diet and folate. The dietary methionine increased aortic lipid peroxidation as shown by the increase in TBARS (604.0 ± 51.0 pmol MDA/mg protein, P < 0.05). Supplementations of folate and TRF at all doses respectively reduced the oxidative stress (300.0 ± 96.0, 334.0 ± 75.0, 338.0 ± 72.0 and 222.0 ± 41.0 pmol/mg protein). High methionine diet also increased expression of VCAM-1 but it was reversed by folate and palm TRF at 150 mg/kg diet. Palm TRF at 150 mg/kg diet and folate reduced oxidative stress and expression of VCAM-1 in the rat aorta probably via reduction of raised plasma homocysteine level induced by high methionine diet in male Wistar rats. 2009-11-18 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/9922/1/australia_2009_poster.pdf Ku Zaifah, Norsidah and Yusuf, Kamisah and Yusuf, Ahmad Asmadi and Ayob, Azizi (2009) The effects of palm tocotrienol rich fraction (TRF) on proatherosclerotic changes in aorta of rats fed high methionine diet. In: 2009 Joanna Briggs Institute International Convention-Ripples to Revolution: From Bench to Bedside, 18 - 20 Nov 2009, Adelaide, South Australia.
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic RM214 Diet therapy. Diet and dietectics in disease
spellingShingle RM214 Diet therapy. Diet and dietectics in disease
Ku Zaifah, Norsidah
Yusuf, Kamisah
Yusuf, Ahmad Asmadi
Ayob, Azizi
The effects of palm tocotrienol rich fraction (TRF) on proatherosclerotic changes in aorta of rats fed high methionine diet
description Hyperhomocysteinemia is a risk factor for cardiovascular diseases. It is associated with an increase in oxidative stress. We aimed to study the effects of palm tocotrienol rich fraction (palm TRF) on proatherosclerotic changes in aorta of rats fed high methionine diet. Methodology:42 male Wistar rats were divided into six groups. The first group was the control. Group 2 to 6 were fed 1% methionine diet for 10 weeks. From week 6 onward, folate (8 mg/kg diet) or palm TRF (30, 60 or 150 mg/kg diet) were added into the diet of group 3 (M+Folate), 4 (M+TRF30), 5 (M+TRF60) and 6 (M+TRF150) respectively. Body weights were recorded weekly. Statistical analysis:Statistical Package for Social Sciences (SPSS) 11.5 was used. Data is presented as Mean ± SEM. P < 0.05 is considered significant. No significant difference in body weight was recorded. Methionine diet (M) significantly raised the total plasma homocysteine (tHcy) level and this was reduced by the supplementations of palm TRF at 150 mg/kg diet and folate. The dietary methionine increased aortic lipid peroxidation as shown by the increase in TBARS (604.0 ± 51.0 pmol MDA/mg protein, P < 0.05). Supplementations of folate and TRF at all doses respectively reduced the oxidative stress (300.0 ± 96.0, 334.0 ± 75.0, 338.0 ± 72.0 and 222.0 ± 41.0 pmol/mg protein). High methionine diet also increased expression of VCAM-1 but it was reversed by folate and palm TRF at 150 mg/kg diet. Palm TRF at 150 mg/kg diet and folate reduced oxidative stress and expression of VCAM-1 in the rat aorta probably via reduction of raised plasma homocysteine level induced by high methionine diet in male Wistar rats.
format Conference or Workshop Item
author Ku Zaifah, Norsidah
Yusuf, Kamisah
Yusuf, Ahmad Asmadi
Ayob, Azizi
author_facet Ku Zaifah, Norsidah
Yusuf, Kamisah
Yusuf, Ahmad Asmadi
Ayob, Azizi
author_sort Ku Zaifah, Norsidah
title The effects of palm tocotrienol rich fraction (TRF) on proatherosclerotic changes in aorta of rats fed high methionine diet
title_short The effects of palm tocotrienol rich fraction (TRF) on proatherosclerotic changes in aorta of rats fed high methionine diet
title_full The effects of palm tocotrienol rich fraction (TRF) on proatherosclerotic changes in aorta of rats fed high methionine diet
title_fullStr The effects of palm tocotrienol rich fraction (TRF) on proatherosclerotic changes in aorta of rats fed high methionine diet
title_full_unstemmed The effects of palm tocotrienol rich fraction (TRF) on proatherosclerotic changes in aorta of rats fed high methionine diet
title_sort effects of palm tocotrienol rich fraction (trf) on proatherosclerotic changes in aorta of rats fed high methionine diet
publishDate 2009
url http://irep.iium.edu.my/9922/
http://irep.iium.edu.my/9922/1/australia_2009_poster.pdf
first_indexed 2023-09-18T20:19:30Z
last_indexed 2023-09-18T20:19:30Z
_version_ 1777408012018778112

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