Evaluation of Antibacterial Activity from Flowering Plants and Optimization of Process Conditions for the Extraction of Antibacterial Compounds from Spathiphyllum Cannifolium Leaves

The screening practices for phytochemical compound from plants is essential to explore more natural sources to replace synthetic antibiotics which generally associated with multi-drug resistance as well as having side effects such as hypersensitivity, immune-suppression and allergic reactions. In th...

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Main Authors:	Abdullah, Erlina, Ahmad Raus, Raha, Jamal, Parveen
Format:	Conference or Workshop Item
Language:	English
Published:	2011
Subjects:	QR Microbiology TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/9808/ http://irep.iium.edu.my/9808/ http://irep.iium.edu.my/9808/1/erin2.pdf

id	iium-9808
recordtype	eprints
spelling	iium-98082011-12-18T01:54:24Z http://irep.iium.edu.my/9808/ Evaluation of Antibacterial Activity from Flowering Plants and Optimization of Process Conditions for the Extraction of Antibacterial Compounds from Spathiphyllum Cannifolium Leaves Abdullah, Erlina Ahmad Raus, Raha Jamal, Parveen QR Microbiology TP248.13 Biotechnology The screening practices for phytochemical compound from plants is essential to explore more natural sources to replace synthetic antibiotics which generally associated with multi-drug resistance as well as having side effects such as hypersensitivity, immune-suppression and allergic reactions. In this study, screening of antibacterial property from 19 Malaysian flowering plants was conducted and optimization of the process conditions for extraction of antibacterial compounds was carried out as well. During the screening, the plants were extracted with methanol, ethyl acetate, hexane and distilled water, individually at concentration of 0.1g/ml for 10 hours at room temperature and agitated at 300 rpm. The crude extracts of each plant (5 mg/disc) were tested against Bacillus subtilis and Escherichia coli using agar disc diffusion assay method. The optimization study was carried out as designed by Central Composite Design (CCD) using Design Expert 6.0.8 software and the parameters that were considered for optimization include agitation, temperature, and incubation time. The screening results showed most of the plant samples extracted with methanol and ethyl acetate, have indicated positive activity toward B. subtilis growth but not E. coli. Ethyl acetate extract of Spathiphyllum cannifolium leaves showed the highest antibacterial activity and optimization of extraction for antibacterial compound was carried out using this plant. The best conditions postulated from optimization study are 10hours, 300rpm, and 30⁰C. Analysis of Variance (ANOVA) of this study demonstrated that both temperature and speed factor significantly (p< 0.05) affects the extraction process. In conclusion, this study suggested S. cannifolium is a highly potential plant to be considered for development of new antibiotic and the optimum extraction process conditions obtained from this study are useful for efficient extraction of antibacterial compounds. 2011 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/9808/1/erin2.pdf Abdullah, Erlina and Ahmad Raus, Raha and Jamal, Parveen (2011) Evaluation of Antibacterial Activity from Flowering Plants and Optimization of Process Conditions for the Extraction of Antibacterial Compounds from Spathiphyllum Cannifolium Leaves. In: 2nd International Conference on Biotechnology Engineering (ICBioE 2011), 17-19 May 2011, The Legend Hotel, Kuala Lumpur. http://www.iium.edu.my/icbioe/2011/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QR Microbiology TP248.13 Biotechnology
spellingShingle	QR Microbiology TP248.13 Biotechnology Abdullah, Erlina Ahmad Raus, Raha Jamal, Parveen Evaluation of Antibacterial Activity from Flowering Plants and Optimization of Process Conditions for the Extraction of Antibacterial Compounds from Spathiphyllum Cannifolium Leaves
description	The screening practices for phytochemical compound from plants is essential to explore more natural sources to replace synthetic antibiotics which generally associated with multi-drug resistance as well as having side effects such as hypersensitivity, immune-suppression and allergic reactions. In this study, screening of antibacterial property from 19 Malaysian flowering plants was conducted and optimization of the process conditions for extraction of antibacterial compounds was carried out as well. During the screening, the plants were extracted with methanol, ethyl acetate, hexane and distilled water, individually at concentration of 0.1g/ml for 10 hours at room temperature and agitated at 300 rpm. The crude extracts of each plant (5 mg/disc) were tested against Bacillus subtilis and Escherichia coli using agar disc diffusion assay method. The optimization study was carried out as designed by Central Composite Design (CCD) using Design Expert 6.0.8 software and the parameters that were considered for optimization include agitation, temperature, and incubation time. The screening results showed most of the plant samples extracted with methanol and ethyl acetate, have indicated positive activity toward B. subtilis growth but not E. coli. Ethyl acetate extract of Spathiphyllum cannifolium leaves showed the highest antibacterial activity and optimization of extraction for antibacterial compound was carried out using this plant. The best conditions postulated from optimization study are 10hours, 300rpm, and 30⁰C. Analysis of Variance (ANOVA) of this study demonstrated that both temperature and speed factor significantly (p< 0.05) affects the extraction process. In conclusion, this study suggested S. cannifolium is a highly potential plant to be considered for development of new antibiotic and the optimum extraction process conditions obtained from this study are useful for efficient extraction of antibacterial compounds.
format	Conference or Workshop Item
author	Abdullah, Erlina Ahmad Raus, Raha Jamal, Parveen
author_facet	Abdullah, Erlina Ahmad Raus, Raha Jamal, Parveen
author_sort	Abdullah, Erlina
title	Evaluation of Antibacterial Activity from Flowering Plants and Optimization of Process Conditions for the Extraction of Antibacterial Compounds from Spathiphyllum Cannifolium Leaves
title_short	Evaluation of Antibacterial Activity from Flowering Plants and Optimization of Process Conditions for the Extraction of Antibacterial Compounds from Spathiphyllum Cannifolium Leaves
title_full	Evaluation of Antibacterial Activity from Flowering Plants and Optimization of Process Conditions for the Extraction of Antibacterial Compounds from Spathiphyllum Cannifolium Leaves
title_fullStr	Evaluation of Antibacterial Activity from Flowering Plants and Optimization of Process Conditions for the Extraction of Antibacterial Compounds from Spathiphyllum Cannifolium Leaves
title_full_unstemmed	Evaluation of Antibacterial Activity from Flowering Plants and Optimization of Process Conditions for the Extraction of Antibacterial Compounds from Spathiphyllum Cannifolium Leaves
title_sort	evaluation of antibacterial activity from flowering plants and optimization of process conditions for the extraction of antibacterial compounds from spathiphyllum cannifolium leaves
publishDate	2011
url	http://irep.iium.edu.my/9808/ http://irep.iium.edu.my/9808/ http://irep.iium.edu.my/9808/1/erin2.pdf
first_indexed	2023-09-18T20:19:25Z
last_indexed	2023-09-18T20:19:25Z
_version_	1777408006687817728

Evaluation of Antibacterial Activity from Flowering Plants and Optimization of Process Conditions for the Extraction of Antibacterial Compounds from Spathiphyllum Cannifolium Leaves

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