Screening and optimization of media components for phytase production of ASUIA 279 in Pichia pastoris system using statistical design

The gene encoding for phytase from ASUIA279 has been successfully cloned and expressed in pichia pastoris X-33 under the control of AOX1 promoter, named as HSUIA279(5H)AX. In this study, process and media components were screened for high yield phytase production in small scale culture by using plac...

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Main Authors:	Mahamad Maifiah, Mohd Hafidz, Mohd. Salleh, Hamzah, Jamal, Parveen, Nuge, Tamrin
Format:	Article
Language:	English
Published:	INSI Publications 2011
Subjects:	TA164 Bioengineering TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/9778/ http://irep.iium.edu.my/9778/ http://irep.iium.edu.my/9778/1/hafidz_paper.pdf

id	iium-9778
recordtype	eprints
spelling	iium-97782011-12-16T08:53:25Z http://irep.iium.edu.my/9778/ Screening and optimization of media components for phytase production of ASUIA 279 in Pichia pastoris system using statistical design Mahamad Maifiah, Mohd Hafidz Mohd. Salleh, Hamzah Jamal, Parveen Nuge, Tamrin TA164 Bioengineering TP248.13 Biotechnology The gene encoding for phytase from ASUIA279 has been successfully cloned and expressed in pichia pastoris X-33 under the control of AOX1 promoter, named as HSUIA279(5H)AX. In this study, process and media components were screened for high yield phytase production in small scale culture by using plackett burman design. Three factors identified (Agitation, YNB, Yeast extract) were further optimized by Response Surface methodology (RSM) using Face Centered Composite Design (FCCD). Phytase activity in the optimized media (28 U/ml) increase 64% higher over than it basal media (17.004 U/ml) in the shaken flask culture. The cloning of the phytase enzyme from ASUIA279 into the Pichia pastoris system with minimal and potential media components would be a significant concern for a cost effective measure to produce a high yield and activity enzyme for animal feed application. INSI Publications 2011 Article PeerReviewed application/pdf en http://irep.iium.edu.my/9778/1/hafidz_paper.pdf Mahamad Maifiah, Mohd Hafidz and Mohd. Salleh, Hamzah and Jamal, Parveen and Nuge, Tamrin (2011) Screening and optimization of media components for phytase production of ASUIA 279 in Pichia pastoris system using statistical design. Australian Journal of Basic and Applied Sciences. ISSN 1991-8178 (In Press) http://www.insipub.com/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TA164 Bioengineering TP248.13 Biotechnology
spellingShingle	TA164 Bioengineering TP248.13 Biotechnology Mahamad Maifiah, Mohd Hafidz Mohd. Salleh, Hamzah Jamal, Parveen Nuge, Tamrin Screening and optimization of media components for phytase production of ASUIA 279 in Pichia pastoris system using statistical design
description	The gene encoding for phytase from ASUIA279 has been successfully cloned and expressed in pichia pastoris X-33 under the control of AOX1 promoter, named as HSUIA279(5H)AX. In this study, process and media components were screened for high yield phytase production in small scale culture by using plackett burman design. Three factors identified (Agitation, YNB, Yeast extract) were further optimized by Response Surface methodology (RSM) using Face Centered Composite Design (FCCD). Phytase activity in the optimized media (28 U/ml) increase 64% higher over than it basal media (17.004 U/ml) in the shaken flask culture. The cloning of the phytase enzyme from ASUIA279 into the Pichia pastoris system with minimal and potential media components would be a significant concern for a cost effective measure to produce a high yield and activity enzyme for animal feed application.
format	Article
author	Mahamad Maifiah, Mohd Hafidz Mohd. Salleh, Hamzah Jamal, Parveen Nuge, Tamrin
author_facet	Mahamad Maifiah, Mohd Hafidz Mohd. Salleh, Hamzah Jamal, Parveen Nuge, Tamrin
author_sort	Mahamad Maifiah, Mohd Hafidz
title	Screening and optimization of media components for phytase production of ASUIA 279 in Pichia pastoris system using statistical design
title_short	Screening and optimization of media components for phytase production of ASUIA 279 in Pichia pastoris system using statistical design
title_full	Screening and optimization of media components for phytase production of ASUIA 279 in Pichia pastoris system using statistical design
title_fullStr	Screening and optimization of media components for phytase production of ASUIA 279 in Pichia pastoris system using statistical design
title_full_unstemmed	Screening and optimization of media components for phytase production of ASUIA 279 in Pichia pastoris system using statistical design
title_sort	screening and optimization of media components for phytase production of asuia 279 in pichia pastoris system using statistical design
publisher	INSI Publications
publishDate	2011
url	http://irep.iium.edu.my/9778/ http://irep.iium.edu.my/9778/ http://irep.iium.edu.my/9778/1/hafidz_paper.pdf
first_indexed	2023-09-18T20:19:24Z
last_indexed	2023-09-18T20:19:24Z
_version_	1777408005221908480

Screening and optimization of media components for phytase production of ASUIA 279 in Pichia pastoris system using statistical design

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