Electron microscopic study of esophageal epithelium in non-erosive reflux disease

Introduction: Non-erosive reflux disease (NERD) is the most common phenotype of gastro esophageal reflux disease (GERD). Dilated intercellular spaces (DIS) in prickle cell layers are considered as early signs of acid damage to the esophageal epithelium. Objectives: The objective of this study was t...

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Main Authors:	Myint, Yi Yi, Tun, Ye, Mohammad Amjad, Nasser, Mat Hassan, Mohd. Zailani, Abd. Rashid, Nor Linda
Format:	Conference or Workshop Item
Language:	English
Published:	2011
Subjects:	R Medicine (General)
Online Access:	http://irep.iium.edu.my/9711/ http://irep.iium.edu.my/9711/2/UITM_final_poster-_YYM.pdf

id	iium-9711
recordtype	eprints
spelling	iium-97112017-06-22T00:23:23Z http://irep.iium.edu.my/9711/ Electron microscopic study of esophageal epithelium in non-erosive reflux disease Myint, Yi Yi Tun, Ye Mohammad Amjad, Nasser Mat Hassan, Mohd. Zailani Abd. Rashid, Nor Linda R Medicine (General) Introduction: Non-erosive reflux disease (NERD) is the most common phenotype of gastro esophageal reflux disease (GERD). Dilated intercellular spaces (DIS) in prickle cell layers are considered as early signs of acid damage to the esophageal epithelium. Objectives: The objective of this study was to explore DIS in both superficial and prickle cell layer of the esophageal epithelium of NERD patients and normal controls by using scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Material and methods: The study group included 5 normal controls and 8 NERD patients with reflux symptoms, normal mucosa on endoscopy and positive 24-hour pH monitoring. Specimens were taken 5 cm above the Z line during gastroduodenoscopy and then routinely processed for both SEM & TEM studies. The nature of intercellular spaces between superficial cells and prickle cell layers were viewed by TEM and SEM respectively. Results: We found out that cellular attachments in the superficial cells differ significantly between the normal controls and patients with NERD (p value 0.007). Morphometric analysis of TEM microphotographs showed that the mean intercellular space diameter values of NERD patients were significantly ( two times) higher than that in normal controls (p<0.001). Conclusions: We can conclude that SEM study on DIS in esophageal surface cells is not a useful diagnostic test for differentiating between normal and NERD patients but TEM study in prickle cell layer is a promising diagnostic finding of early damage of esophageal epithelium. 2011-06-08 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/9711/2/UITM_final_poster-_YYM.pdf Myint, Yi Yi and Tun, Ye and Mohammad Amjad, Nasser and Mat Hassan, Mohd. Zailani and Abd. Rashid, Nor Linda (2011) Electron microscopic study of esophageal epithelium in non-erosive reflux disease. In: 2nd Malaysian Anatomical Conference, 8 - 9 June, 2011, Selangor, Malaysia. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	R Medicine (General)
spellingShingle	R Medicine (General) Myint, Yi Yi Tun, Ye Mohammad Amjad, Nasser Mat Hassan, Mohd. Zailani Abd. Rashid, Nor Linda Electron microscopic study of esophageal epithelium in non-erosive reflux disease
description	Introduction: Non-erosive reflux disease (NERD) is the most common phenotype of gastro esophageal reflux disease (GERD). Dilated intercellular spaces (DIS) in prickle cell layers are considered as early signs of acid damage to the esophageal epithelium. Objectives: The objective of this study was to explore DIS in both superficial and prickle cell layer of the esophageal epithelium of NERD patients and normal controls by using scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Material and methods: The study group included 5 normal controls and 8 NERD patients with reflux symptoms, normal mucosa on endoscopy and positive 24-hour pH monitoring. Specimens were taken 5 cm above the Z line during gastroduodenoscopy and then routinely processed for both SEM & TEM studies. The nature of intercellular spaces between superficial cells and prickle cell layers were viewed by TEM and SEM respectively. Results: We found out that cellular attachments in the superficial cells differ significantly between the normal controls and patients with NERD (p value 0.007). Morphometric analysis of TEM microphotographs showed that the mean intercellular space diameter values of NERD patients were significantly ( two times) higher than that in normal controls (p<0.001). Conclusions: We can conclude that SEM study on DIS in esophageal surface cells is not a useful diagnostic test for differentiating between normal and NERD patients but TEM study in prickle cell layer is a promising diagnostic finding of early damage of esophageal epithelium.
format	Conference or Workshop Item
author	Myint, Yi Yi Tun, Ye Mohammad Amjad, Nasser Mat Hassan, Mohd. Zailani Abd. Rashid, Nor Linda
author_facet	Myint, Yi Yi Tun, Ye Mohammad Amjad, Nasser Mat Hassan, Mohd. Zailani Abd. Rashid, Nor Linda
author_sort	Myint, Yi Yi
title	Electron microscopic study of esophageal epithelium in non-erosive reflux disease
title_short	Electron microscopic study of esophageal epithelium in non-erosive reflux disease
title_full	Electron microscopic study of esophageal epithelium in non-erosive reflux disease
title_fullStr	Electron microscopic study of esophageal epithelium in non-erosive reflux disease
title_full_unstemmed	Electron microscopic study of esophageal epithelium in non-erosive reflux disease
title_sort	electron microscopic study of esophageal epithelium in non-erosive reflux disease
publishDate	2011
url	http://irep.iium.edu.my/9711/ http://irep.iium.edu.my/9711/2/UITM_final_poster-_YYM.pdf
first_indexed	2023-09-18T20:19:19Z
last_indexed	2023-09-18T20:19:19Z
_version_	1777408000007340032

Electron microscopic study of esophageal epithelium in non-erosive reflux disease

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