The protective effect of sodium nitroprusside(SNP)treatment on Arabidopsis thaliana seedlings exposed to toxic level of Pb is not linked to avoidance of Pb uptake

The effects of 100 μM Pb(NO3)2 on 7-day-old Arabidopsis thaliana seedlings grown from seeds pretreated with a nitric oxide donor, sodium nitroprusside (SNP), or a nitric oxide scavenger, 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (cPTIO) were investigated. Physiological stress...

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Main Authors:	Phang, Ing Chia, Leung, David W. M., Taylor, H. Harry, Burritt, David J.
Format:	Article
Language:	English
Published:	Academic Press 2011
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/938/ http://irep.iium.edu.my/938/ http://irep.iium.edu.my/938/ http://irep.iium.edu.my/938/2/Phang_et_al_2011.PDF

id	iium-938
recordtype	eprints
spelling	iium-9382013-06-21T03:40:57Z http://irep.iium.edu.my/938/ The protective effect of sodium nitroprusside(SNP)treatment on Arabidopsis thaliana seedlings exposed to toxic level of Pb is not linked to avoidance of Pb uptake Phang, Ing Chia Leung, David W. M. Taylor, H. Harry Burritt, David J. Q Science (General) The effects of 100 μM Pb(NO3)2 on 7-day-old Arabidopsis thaliana seedlings grown from seeds pretreated with a nitric oxide donor, sodium nitroprusside (SNP), or a nitric oxide scavenger, 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (cPTIO) were investigated. Physiological stress induced by Pb (reduced root growth) was less evident in seedlings grown from seeds pretreated with SNP. However, SNP pretreatment of seeds did not affect Pb accumulation in the seedlings. Pb exposure caused oxidative stress by elevating hydrogen peroxide (H2O2) and lipid hydroperoxide contents of the seedlings. SNP pretreatment of seeds counteracted Pb toxicity by reducing the H2O2 and lipid hydroperoxide contents of Pb-exposed seedlings. Additionally, Pb-induced rises in antioxidative enzyme activities were reversed by SNP pretreatment of seeds. It was concluded that the ameliorating effects of SNP pretreatment were associated with the release of nitric oxide because cPTIO reversed these effects of SNP pretreatment of seeds and more importantly SNP pretreatment did not trigger an avoidance mechanism. Academic Press 2011-07 Article PeerReviewed application/pdf en http://irep.iium.edu.my/938/2/Phang_et_al_2011.PDF Phang, Ing Chia and Leung, David W. M. and Taylor, H. Harry and Burritt, David J. (2011) The protective effect of sodium nitroprusside(SNP)treatment on Arabidopsis thaliana seedlings exposed to toxic level of Pb is not linked to avoidance of Pb uptake. Ecotoxicology and Environmental Safety, 74 (5). pp. 1310-1315. ISSN 0147-6513 http://dx.doi.org/10.1016/j.ecoenv.2011.02.006 doi:10.1016/j.ecoenv.2011.02.006
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Phang, Ing Chia Leung, David W. M. Taylor, H. Harry Burritt, David J. The protective effect of sodium nitroprusside(SNP)treatment on Arabidopsis thaliana seedlings exposed to toxic level of Pb is not linked to avoidance of Pb uptake
description	The effects of 100 μM Pb(NO3)2 on 7-day-old Arabidopsis thaliana seedlings grown from seeds pretreated with a nitric oxide donor, sodium nitroprusside (SNP), or a nitric oxide scavenger, 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (cPTIO) were investigated. Physiological stress induced by Pb (reduced root growth) was less evident in seedlings grown from seeds pretreated with SNP. However, SNP pretreatment of seeds did not affect Pb accumulation in the seedlings. Pb exposure caused oxidative stress by elevating hydrogen peroxide (H2O2) and lipid hydroperoxide contents of the seedlings. SNP pretreatment of seeds counteracted Pb toxicity by reducing the H2O2 and lipid hydroperoxide contents of Pb-exposed seedlings. Additionally, Pb-induced rises in antioxidative enzyme activities were reversed by SNP pretreatment of seeds. It was concluded that the ameliorating effects of SNP pretreatment were associated with the release of nitric oxide because cPTIO reversed these effects of SNP pretreatment of seeds and more importantly SNP pretreatment did not trigger an avoidance mechanism.
format	Article
author	Phang, Ing Chia Leung, David W. M. Taylor, H. Harry Burritt, David J.
author_facet	Phang, Ing Chia Leung, David W. M. Taylor, H. Harry Burritt, David J.
author_sort	Phang, Ing Chia
title	The protective effect of sodium nitroprusside(SNP)treatment on Arabidopsis thaliana seedlings exposed to toxic level of Pb is not linked to avoidance of Pb uptake
title_short	The protective effect of sodium nitroprusside(SNP)treatment on Arabidopsis thaliana seedlings exposed to toxic level of Pb is not linked to avoidance of Pb uptake
title_full	The protective effect of sodium nitroprusside(SNP)treatment on Arabidopsis thaliana seedlings exposed to toxic level of Pb is not linked to avoidance of Pb uptake
title_fullStr	The protective effect of sodium nitroprusside(SNP)treatment on Arabidopsis thaliana seedlings exposed to toxic level of Pb is not linked to avoidance of Pb uptake
title_full_unstemmed	The protective effect of sodium nitroprusside(SNP)treatment on Arabidopsis thaliana seedlings exposed to toxic level of Pb is not linked to avoidance of Pb uptake
title_sort	protective effect of sodium nitroprusside(snp)treatment on arabidopsis thaliana seedlings exposed to toxic level of pb is not linked to avoidance of pb uptake
publisher	Academic Press
publishDate	2011
url	http://irep.iium.edu.my/938/ http://irep.iium.edu.my/938/ http://irep.iium.edu.my/938/ http://irep.iium.edu.my/938/2/Phang_et_al_2011.PDF
first_indexed	2023-09-18T20:08:07Z
last_indexed	2023-09-18T20:08:07Z
_version_	1777407295824592896

The protective effect of sodium nitroprusside(SNP)treatment on Arabidopsis thaliana seedlings exposed to toxic level of Pb is not linked to avoidance of Pb uptake

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