Optimization of the elicitation process on chrysanthemum indicum cell suspension culture producing xanthine oxidase inhibitor

This research reports the study that aims to enhance the activity of xanthine oxidase inhibitor, a secondary metabolite, found in Chrysanthemum indicum and to identify the most suitable elicitors with the optimize elicitation time and concentration. Many plant species are well known source of second...

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Main Authors:	Amid, Azura, Jamal, Parveen
Format:	Article
Language:	English
Published:	Asian Network for Scientific Information 2009
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/867/ http://irep.iium.edu.my/867/ http://irep.iium.edu.my/867/ http://irep.iium.edu.my/867/1/2256-2263.pdf

id	iium-867
recordtype	eprints
spelling	iium-8672011-07-18T03:48:50Z http://irep.iium.edu.my/867/ Optimization of the elicitation process on chrysanthemum indicum cell suspension culture producing xanthine oxidase inhibitor Amid, Azura Jamal, Parveen TP248.13 Biotechnology This research reports the study that aims to enhance the activity of xanthine oxidase inhibitor, a secondary metabolite, found in Chrysanthemum indicum and to identify the most suitable elicitors with the optimize elicitation time and concentration. Many plant species are well known source of secondary metabolite with a variety of biological activities. Production of biologically active compounds using plant cell suspension culture approach, normally gives low result. Optimization studies to enhance xanthine oxidase inhibitor activity using different elicitor concentrations for three different types of elicitor (chitosan, yeast extract and Aspergilus niger) were investigated. A factorial design was used from MINITAB software for optimization of the process. Analysis of results proved that chitosan is the best elicitor to increase activity of xanthine oxidase inhibitor in C. indicum to 1.457 fold higher than the unelicited. The highest xanthine oxidase activity was obtained by chitosan elicitation at concentration of 0.16 g L-1 and it was elicited at 1.25 day cultivation or at 30 h culture with percentage inhibition of 61.818%. Asian Network for Scientific Information 2009 Article PeerReviewed application/pdf en http://irep.iium.edu.my/867/1/2256-2263.pdf Amid, Azura and Jamal, Parveen (2009) Optimization of the elicitation process on chrysanthemum indicum cell suspension culture producing xanthine oxidase inhibitor. Journal of Applied Sciences, 9 (12). pp. 2256-2263. ISSN 1812-5662 (O), 1812-5654 (P) http://scialert.net/abstract/?doi=jas.2009.2256.2263 DOI: 10.3923/jas.2009.2256.2263
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Amid, Azura Jamal, Parveen Optimization of the elicitation process on chrysanthemum indicum cell suspension culture producing xanthine oxidase inhibitor
description	This research reports the study that aims to enhance the activity of xanthine oxidase inhibitor, a secondary metabolite, found in Chrysanthemum indicum and to identify the most suitable elicitors with the optimize elicitation time and concentration. Many plant species are well known source of secondary metabolite with a variety of biological activities. Production of biologically active compounds using plant cell suspension culture approach, normally gives low result. Optimization studies to enhance xanthine oxidase inhibitor activity using different elicitor concentrations for three different types of elicitor (chitosan, yeast extract and Aspergilus niger) were investigated. A factorial design was used from MINITAB software for optimization of the process. Analysis of results proved that chitosan is the best elicitor to increase activity of xanthine oxidase inhibitor in C. indicum to 1.457 fold higher than the unelicited. The highest xanthine oxidase activity was obtained by chitosan elicitation at concentration of 0.16 g L-1 and it was elicited at 1.25 day cultivation or at 30 h culture with percentage inhibition of 61.818%.
format	Article
author	Amid, Azura Jamal, Parveen
author_facet	Amid, Azura Jamal, Parveen
author_sort	Amid, Azura
title	Optimization of the elicitation process on chrysanthemum indicum cell suspension culture producing xanthine oxidase inhibitor
title_short	Optimization of the elicitation process on chrysanthemum indicum cell suspension culture producing xanthine oxidase inhibitor
title_full	Optimization of the elicitation process on chrysanthemum indicum cell suspension culture producing xanthine oxidase inhibitor
title_fullStr	Optimization of the elicitation process on chrysanthemum indicum cell suspension culture producing xanthine oxidase inhibitor
title_full_unstemmed	Optimization of the elicitation process on chrysanthemum indicum cell suspension culture producing xanthine oxidase inhibitor
title_sort	optimization of the elicitation process on chrysanthemum indicum cell suspension culture producing xanthine oxidase inhibitor
publisher	Asian Network for Scientific Information
publishDate	2009
url	http://irep.iium.edu.my/867/ http://irep.iium.edu.my/867/ http://irep.iium.edu.my/867/ http://irep.iium.edu.my/867/1/2256-2263.pdf
first_indexed	2023-09-18T20:08:03Z
last_indexed	2023-09-18T20:08:03Z
_version_	1777407291270627328

Optimization of the elicitation process on chrysanthemum indicum cell suspension culture producing xanthine oxidase inhibitor

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