Bioautographic screening for natural quinolone antimicrobial agents from Glycosmis pentaphylla (Retz.) DC., Ruta angustifolia (L.) Pers. and Lunasia amara Blanco

Background The quinoline alkaloids are a group of alkaloids with diverse structural types. The 4-oxygenated quinolone and 2-substituted 4-quinolone are the types of quinoline alkaloids that possess the chromophore structures which are similar to the pharmacophore of the conventional 4-quinolone a...

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Main Authors:	Md Taib, Nurhaya, Mohd Kamal, Laina Zarisa, Mohd Hassan, Norazian, Khin Soe, May, Abdul Karim, Khairul Anuar
Format:	Conference or Workshop Item
Language:	English
Published:	2009
Subjects:	QK Botany QR180 Immunology RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/8387/ http://irep.iium.edu.my/8387/ http://irep.iium.edu.my/8387/1/POSTER.pdf

id	iium-8387
recordtype	eprints
spelling	iium-83872011-12-12T03:31:42Z http://irep.iium.edu.my/8387/ Bioautographic screening for natural quinolone antimicrobial agents from Glycosmis pentaphylla (Retz.) DC., Ruta angustifolia (L.) Pers. and Lunasia amara Blanco Md Taib, Nurhaya Mohd Kamal, Laina Zarisa Mohd Hassan, Norazian Khin Soe, May Abdul Karim, Khairul Anuar QK Botany QR180 Immunology RS Pharmacy and materia medica Background The quinoline alkaloids are a group of alkaloids with diverse structural types. The 4-oxygenated quinolone and 2-substituted 4-quinolone are the types of quinoline alkaloids that possess the chromophore structures which are similar to the pharmacophore of the conventional 4-quinolone antimicrobial agents. Therefore it is presumed that these types of natural quinoline alkaloids could also share the same activity as the conventional quinolone antimicrobial agents. Although these conventional agents demonstrate an excellent treatment against so many infectious diseases, the emergence of quinolone resistance against some microbes has been a disturbing feature of microbial infection (Shigemura, et al., 2003). Objectives This study aims primarily to search for naturally occurring quinolone antimicrobial agents from three excellent plant sources of quinolone alkaloids, which are Glycosmis pentaphylla (Retz.) DC., Ruta angustifolia (L.) Pers. and Lunasia amara Blanco. It is aimed that the natural alkaloids will provide new structural types of quinolone antimicrobial agents in view to overcome microbial resistance towards the conventional agents. Methods The plant materials, which include the leaves and root barks of G. pentaphylla, and the leaves of R. angustifolia and L. amara were dried and powdered and subjected to extraction. Acid base extraction was employed following the continuous extraction by hexane and acetone to furnish the crude alkaloidal extracts. The extract was then fractionated by column chromatography. The fractions yielding major alkaloidal compounds were selected to be screened by using TLC Agar Overlay Bioautographic Assay for antimicrobial active quinolone alkaloids against Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922 and Candida albicans ATCC 90028. Results The column chromatography on the crude alkaloidal extracts has afforded two fractions containing major alkaloids from G. pentaphylla and one fraction each from R. angustifolia and L. amara. TLC Agar Overlay Bioautographic screening for antimicrobial active alkaloids has revealed four antimicrobial active alkaloids. Two alkaloids, labeled as GP-3 and RA-3 which were active against S. aureus and C. albicans have been detected from the stem bark fraction of G. pentaphylla and the leaf fraction of R. angustifolia. Two major alkaloids such as GP-4 from the leaf fraction of G. pentaphylla and LA-4 from L. amara leaf fraction were found to be active against both S. aureus and E. coli. Conclusion The reported results of all three plant species therefore proved to have high potential in providing new structural types of lead compounds for synthesizing new drugs to overcome microbial resistance towards conventional quinolone antimicrobial agents. 2009-11-09 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/8387/1/POSTER.pdf Md Taib, Nurhaya and Mohd Kamal, Laina Zarisa and Mohd Hassan, Norazian and Khin Soe, May and Abdul Karim, Khairul Anuar (2009) Bioautographic screening for natural quinolone antimicrobial agents from Glycosmis pentaphylla (Retz.) DC., Ruta angustifolia (L.) Pers. and Lunasia amara Blanco. In: 4th National Medical Microbiology Seminar and Workshop 2009: Advanced Techniques on Multiple Drug Resistance (MDR) Epidemiology and Genetics, 9 Nov 2009, Serdang, Malaysia. (Unpublished) http://medic1.upm.edu.my/MDTBP
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QK Botany QR180 Immunology RS Pharmacy and materia medica
spellingShingle	QK Botany QR180 Immunology RS Pharmacy and materia medica Md Taib, Nurhaya Mohd Kamal, Laina Zarisa Mohd Hassan, Norazian Khin Soe, May Abdul Karim, Khairul Anuar Bioautographic screening for natural quinolone antimicrobial agents from Glycosmis pentaphylla (Retz.) DC., Ruta angustifolia (L.) Pers. and Lunasia amara Blanco
description	Background The quinoline alkaloids are a group of alkaloids with diverse structural types. The 4-oxygenated quinolone and 2-substituted 4-quinolone are the types of quinoline alkaloids that possess the chromophore structures which are similar to the pharmacophore of the conventional 4-quinolone antimicrobial agents. Therefore it is presumed that these types of natural quinoline alkaloids could also share the same activity as the conventional quinolone antimicrobial agents. Although these conventional agents demonstrate an excellent treatment against so many infectious diseases, the emergence of quinolone resistance against some microbes has been a disturbing feature of microbial infection (Shigemura, et al., 2003). Objectives This study aims primarily to search for naturally occurring quinolone antimicrobial agents from three excellent plant sources of quinolone alkaloids, which are Glycosmis pentaphylla (Retz.) DC., Ruta angustifolia (L.) Pers. and Lunasia amara Blanco. It is aimed that the natural alkaloids will provide new structural types of quinolone antimicrobial agents in view to overcome microbial resistance towards the conventional agents. Methods The plant materials, which include the leaves and root barks of G. pentaphylla, and the leaves of R. angustifolia and L. amara were dried and powdered and subjected to extraction. Acid base extraction was employed following the continuous extraction by hexane and acetone to furnish the crude alkaloidal extracts. The extract was then fractionated by column chromatography. The fractions yielding major alkaloidal compounds were selected to be screened by using TLC Agar Overlay Bioautographic Assay for antimicrobial active quinolone alkaloids against Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922 and Candida albicans ATCC 90028. Results The column chromatography on the crude alkaloidal extracts has afforded two fractions containing major alkaloids from G. pentaphylla and one fraction each from R. angustifolia and L. amara. TLC Agar Overlay Bioautographic screening for antimicrobial active alkaloids has revealed four antimicrobial active alkaloids. Two alkaloids, labeled as GP-3 and RA-3 which were active against S. aureus and C. albicans have been detected from the stem bark fraction of G. pentaphylla and the leaf fraction of R. angustifolia. Two major alkaloids such as GP-4 from the leaf fraction of G. pentaphylla and LA-4 from L. amara leaf fraction were found to be active against both S. aureus and E. coli. Conclusion The reported results of all three plant species therefore proved to have high potential in providing new structural types of lead compounds for synthesizing new drugs to overcome microbial resistance towards conventional quinolone antimicrobial agents.
format	Conference or Workshop Item
author	Md Taib, Nurhaya Mohd Kamal, Laina Zarisa Mohd Hassan, Norazian Khin Soe, May Abdul Karim, Khairul Anuar
author_facet	Md Taib, Nurhaya Mohd Kamal, Laina Zarisa Mohd Hassan, Norazian Khin Soe, May Abdul Karim, Khairul Anuar
author_sort	Md Taib, Nurhaya
title	Bioautographic screening for natural quinolone antimicrobial agents from Glycosmis pentaphylla (Retz.) DC., Ruta angustifolia (L.) Pers. and Lunasia amara Blanco
title_short	Bioautographic screening for natural quinolone antimicrobial agents from Glycosmis pentaphylla (Retz.) DC., Ruta angustifolia (L.) Pers. and Lunasia amara Blanco
title_full	Bioautographic screening for natural quinolone antimicrobial agents from Glycosmis pentaphylla (Retz.) DC., Ruta angustifolia (L.) Pers. and Lunasia amara Blanco
title_fullStr	Bioautographic screening for natural quinolone antimicrobial agents from Glycosmis pentaphylla (Retz.) DC., Ruta angustifolia (L.) Pers. and Lunasia amara Blanco
title_full_unstemmed	Bioautographic screening for natural quinolone antimicrobial agents from Glycosmis pentaphylla (Retz.) DC., Ruta angustifolia (L.) Pers. and Lunasia amara Blanco
title_sort	bioautographic screening for natural quinolone antimicrobial agents from glycosmis pentaphylla (retz.) dc., ruta angustifolia (l.) pers. and lunasia amara blanco
publishDate	2009
url	http://irep.iium.edu.my/8387/ http://irep.iium.edu.my/8387/ http://irep.iium.edu.my/8387/1/POSTER.pdf
first_indexed	2023-09-18T20:18:04Z
last_indexed	2023-09-18T20:18:04Z
_version_	1777407921572806656

Bioautographic screening for natural quinolone antimicrobial agents from Glycosmis pentaphylla (Retz.) DC., Ruta angustifolia (L.) Pers. and Lunasia amara Blanco

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