Antidiabetic activities of Cinnamomum zeylanicum and Tinospora crispa on adipocytes

The effects of Cinnamomum burmannii and Tinospora crispa on differentiation and glucose uptake in 3T3-L1 adipocyte cells were studied. Cells were stimulated to differentiate with differentiation cocktail containing Dulbecco’s modified Eagle’s medium (DMEM) with 10% fetal bovine serum (FBS), 0.25 µM...

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Main Authors: Bakhtiar, M. Taher, Mohamed Amiroudine, Mohamed Zaffar Ali, Solachuddin Jauhari, Arief Ichwan, Kaderi, Mohd Arifin, Deny Susanti, Darnis
Format: Conference or Workshop Item
Language:English
Published: 2011
Subjects:
Online Access:http://irep.iium.edu.my/8306/
http://irep.iium.edu.my/8306/
http://irep.iium.edu.my/8306/1/antidiabetic.pdf
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recordtype eprints
spelling iium-83062014-12-09T01:30:43Z http://irep.iium.edu.my/8306/ Antidiabetic activities of Cinnamomum zeylanicum and Tinospora crispa on adipocytes Bakhtiar, M. Taher Mohamed Amiroudine, Mohamed Zaffar Ali Solachuddin Jauhari, Arief Ichwan Kaderi, Mohd Arifin Deny Susanti, Darnis RS Pharmacy and materia medica The effects of Cinnamomum burmannii and Tinospora crispa on differentiation and glucose uptake in 3T3-L1 adipocyte cells were studied. Cells were stimulated to differentiate with differentiation cocktail containing Dulbecco’s modified Eagle’s medium (DMEM) with 10% fetal bovine serum (FBS), 0.25 µM dexamethasone, 0.5 mM 3-isobutyl-1-methyl-xanthine and 167 µM insulin. 3T3-L1 preadipocytes were treated with various concentrations of the extracts in the presence of differentiation cocktail. Insulin was used as a positive control. The ability of the extracts to induce differentiation in preadipocytes was examined by measuring fat accumulation using oil red O staining. Our results showed that C. burmannii and T. crispa was statistically significant in inducing preadipocyte into mature adipocyte at dose of 0.1 mg/mL (p < 0.001) and 0.5 mg/mL (p < 0.05), respectively. Glucose uptake activity was investigated in differentiated adipocytes using a radioactive-labelled glucose by Liquid Scintillation Counter. The activities in the glucose uptake between untreated and treated cells showed that both plants were statistically significant compared metformin (1 mM), but not significant compared to sodium orthovanadate (5 mM). In conclusion, these results suggest that both plants were effective in inducing adipocyte differentiation and stimulate glucose uptake. 2011 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/8306/1/antidiabetic.pdf Bakhtiar, M. Taher and Mohamed Amiroudine, Mohamed Zaffar Ali and Solachuddin Jauhari, Arief Ichwan and Kaderi, Mohd Arifin and Deny Susanti, Darnis (2011) Antidiabetic activities of Cinnamomum zeylanicum and Tinospora crispa on adipocytes. In: International Conference on Natural products (ICNP 2011), 14-16 November 2011, Palm garden Resort. http://www.icnp2011.upm.edu.my/
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic RS Pharmacy and materia medica
spellingShingle RS Pharmacy and materia medica
Bakhtiar, M. Taher
Mohamed Amiroudine, Mohamed Zaffar Ali
Solachuddin Jauhari, Arief Ichwan
Kaderi, Mohd Arifin
Deny Susanti, Darnis
Antidiabetic activities of Cinnamomum zeylanicum and Tinospora crispa on adipocytes
description The effects of Cinnamomum burmannii and Tinospora crispa on differentiation and glucose uptake in 3T3-L1 adipocyte cells were studied. Cells were stimulated to differentiate with differentiation cocktail containing Dulbecco’s modified Eagle’s medium (DMEM) with 10% fetal bovine serum (FBS), 0.25 µM dexamethasone, 0.5 mM 3-isobutyl-1-methyl-xanthine and 167 µM insulin. 3T3-L1 preadipocytes were treated with various concentrations of the extracts in the presence of differentiation cocktail. Insulin was used as a positive control. The ability of the extracts to induce differentiation in preadipocytes was examined by measuring fat accumulation using oil red O staining. Our results showed that C. burmannii and T. crispa was statistically significant in inducing preadipocyte into mature adipocyte at dose of 0.1 mg/mL (p < 0.001) and 0.5 mg/mL (p < 0.05), respectively. Glucose uptake activity was investigated in differentiated adipocytes using a radioactive-labelled glucose by Liquid Scintillation Counter. The activities in the glucose uptake between untreated and treated cells showed that both plants were statistically significant compared metformin (1 mM), but not significant compared to sodium orthovanadate (5 mM). In conclusion, these results suggest that both plants were effective in inducing adipocyte differentiation and stimulate glucose uptake.
format Conference or Workshop Item
author Bakhtiar, M. Taher
Mohamed Amiroudine, Mohamed Zaffar Ali
Solachuddin Jauhari, Arief Ichwan
Kaderi, Mohd Arifin
Deny Susanti, Darnis
author_facet Bakhtiar, M. Taher
Mohamed Amiroudine, Mohamed Zaffar Ali
Solachuddin Jauhari, Arief Ichwan
Kaderi, Mohd Arifin
Deny Susanti, Darnis
author_sort Bakhtiar, M. Taher
title Antidiabetic activities of Cinnamomum zeylanicum and Tinospora crispa on adipocytes
title_short Antidiabetic activities of Cinnamomum zeylanicum and Tinospora crispa on adipocytes
title_full Antidiabetic activities of Cinnamomum zeylanicum and Tinospora crispa on adipocytes
title_fullStr Antidiabetic activities of Cinnamomum zeylanicum and Tinospora crispa on adipocytes
title_full_unstemmed Antidiabetic activities of Cinnamomum zeylanicum and Tinospora crispa on adipocytes
title_sort antidiabetic activities of cinnamomum zeylanicum and tinospora crispa on adipocytes
publishDate 2011
url http://irep.iium.edu.my/8306/
http://irep.iium.edu.my/8306/
http://irep.iium.edu.my/8306/1/antidiabetic.pdf
first_indexed 2023-09-18T20:17:59Z
last_indexed 2023-09-18T20:17:59Z
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