Effects of coated capillary column, derivatization, and temperature programming on the identification of Carica papaya seed extract composition using GC/MS analysis

Effects of coated capillary column, derivatization, and temperature programming on the identification of Carica papaya seed extract composition using GC/MS analysis

Some of the common practices to assess the composition of plant extract, including Carica papaya seed extract (CPSE) are direct injection of the extract, compound separation using polyethylene glycol capillary column (DB-WAX), and non-linear- temperature programming (NLTP) of GC/MS analysis. This st...

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Main Authors: Abdullah Sani, Muhamad Shirwan, Bakar, Jamilah, Abdul Rahman, Russly, Abas, Faridah
Format: Article
Language:English
Published: Springer Link 2020
Subjects:
QD Chemistry
Online Access:http://irep.iium.edu.my/79437/
http://irep.iium.edu.my/79437/
http://irep.iium.edu.my/79437/
http://irep.iium.edu.my/79437/1/Sani%20et%20al.%20-%202020%20-%20Effects%20of%20coated%20capillary%20column%2C%20derivatization%2C%20and%20temperature%20programming%20on%20the%20identification%20of%20Carica%20pap.pdf
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recordtype eprints
spelling iium-794372020-03-10T01:15:15Z http://irep.iium.edu.my/79437/ Effects of coated capillary column, derivatization, and temperature programming on the identification of Carica papaya seed extract composition using GC/MS analysis Abdullah Sani, Muhamad Shirwan Bakar, Jamilah Abdul Rahman, Russly Abas, Faridah QD Chemistry Some of the common practices to assess the composition of plant extract, including Carica papaya seed extract (CPSE) are direct injection of the extract, compound separation using polyethylene glycol capillary column (DB-WAX), and non-linear- temperature programming (NLTP) of GC/MS analysis. This study specifically compared the coating of capillary column, sample derivatization, and temperature programming of GC/MS to determine the composition of CPSE. The retention indices (RI) of the detected compounds were determined and compared to the reference RI. In particular, 5% phenyl–95% methylpolysiloxane (HP-5MS)-, DB-WAX-, and biscyanopropyl polysiloxane (HP-88)-coated capillary columns were used to identify the composition of CPSE. For this study, HP-5MS column, which separated the highest number of compounds (26 compounds) from CPSE, was deemed as the most suitable column. The GC/MS analysis of derivatized CPSE identified 21 compound groups, where fatty acids and fatty acid methyl esters served as the major compounds (80.23%), followed by these compounds in decreasing order: amides > nitriles > sterols > fatty aldehydes > organic acids. A stronger correlation determination between the carbon number and alkane retention time of linear-temperature programming (LTP) (R2 = 0.9859) was found, as compared to its correlation determination with NLTP (R2 = 0.9175), which exhibited an almost equal RI of LTP to the reference RI. Conclusively, GC/MS analysis for the derivatized CPSE using HP-5MS column separation and LTP is highly recommended. Springer Link 2020-03-05 Article PeerReviewed application/pdf en http://irep.iium.edu.my/79437/1/Sani%20et%20al.%20-%202020%20-%20Effects%20of%20coated%20capillary%20column%2C%20derivatization%2C%20and%20temperature%20programming%20on%20the%20identification%20of%20Carica%20pap.pdf Abdullah Sani, Muhamad Shirwan and Bakar, Jamilah and Abdul Rahman, Russly and Abas, Faridah (2020) Effects of coated capillary column, derivatization, and temperature programming on the identification of Carica papaya seed extract composition using GC/MS analysis. Journal of Analysis and Testing. pp. 1-12. ISSN 2096-241X E-ISSN 2509-4696 (In Press) https://link.springer.com/article/10.1007/s41664-020-00118-z 10.1007/s41664-020-00118-z
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic QD Chemistry
spellingShingle QD Chemistry
Abdullah Sani, Muhamad Shirwan
Bakar, Jamilah
Abdul Rahman, Russly
Abas, Faridah
Effects of coated capillary column, derivatization, and temperature programming on the identification of Carica papaya seed extract composition using GC/MS analysis
description Some of the common practices to assess the composition of plant extract, including Carica papaya seed extract (CPSE) are direct injection of the extract, compound separation using polyethylene glycol capillary column (DB-WAX), and non-linear- temperature programming (NLTP) of GC/MS analysis. This study specifically compared the coating of capillary column, sample derivatization, and temperature programming of GC/MS to determine the composition of CPSE. The retention indices (RI) of the detected compounds were determined and compared to the reference RI. In particular, 5% phenyl–95% methylpolysiloxane (HP-5MS)-, DB-WAX-, and biscyanopropyl polysiloxane (HP-88)-coated capillary columns were used to identify the composition of CPSE. For this study, HP-5MS column, which separated the highest number of compounds (26 compounds) from CPSE, was deemed as the most suitable column. The GC/MS analysis of derivatized CPSE identified 21 compound groups, where fatty acids and fatty acid methyl esters served as the major compounds (80.23%), followed by these compounds in decreasing order: amides > nitriles > sterols > fatty aldehydes > organic acids. A stronger correlation determination between the carbon number and alkane retention time of linear-temperature programming (LTP) (R2 = 0.9859) was found, as compared to its correlation determination with NLTP (R2 = 0.9175), which exhibited an almost equal RI of LTP to the reference RI. Conclusively, GC/MS analysis for the derivatized CPSE using HP-5MS column separation and LTP is highly recommended.
format Article
author Abdullah Sani, Muhamad Shirwan
Bakar, Jamilah
Abdul Rahman, Russly
Abas, Faridah
author_facet Abdullah Sani, Muhamad Shirwan
Bakar, Jamilah
Abdul Rahman, Russly
Abas, Faridah
author_sort Abdullah Sani, Muhamad Shirwan
title Effects of coated capillary column, derivatization, and temperature programming on the identification of Carica papaya seed extract composition using GC/MS analysis
title_short Effects of coated capillary column, derivatization, and temperature programming on the identification of Carica papaya seed extract composition using GC/MS analysis
title_full Effects of coated capillary column, derivatization, and temperature programming on the identification of Carica papaya seed extract composition using GC/MS analysis
title_fullStr Effects of coated capillary column, derivatization, and temperature programming on the identification of Carica papaya seed extract composition using GC/MS analysis
title_full_unstemmed Effects of coated capillary column, derivatization, and temperature programming on the identification of Carica papaya seed extract composition using GC/MS analysis
title_sort effects of coated capillary column, derivatization, and temperature programming on the identification of carica papaya seed extract composition using gc/ms analysis
publisher Springer Link
publishDate 2020
url http://irep.iium.edu.my/79437/
http://irep.iium.edu.my/79437/
http://irep.iium.edu.my/79437/
http://irep.iium.edu.my/79437/1/Sani%20et%20al.%20-%202020%20-%20Effects%20of%20coated%20capillary%20column%2C%20derivatization%2C%20and%20temperature%20programming%20on%20the%20identification%20of%20Carica%20pap.pdf
first_indexed 2023-09-18T21:51:29Z
last_indexed 2023-09-18T21:51:29Z
_version_ 1777413799081410560

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