Optimization of bio-active compounds from stem of Anisophyllea disticha using supercritical fluid extraction technique

Background: Anisophyllea disticha is belonging to the species of genus Anisophyllea, is not pharmacologically investigated despite its high utilization by the folklore in treating diarrhea, dysentery, jaundice, revitalizing the birth canal of women, relieving weariness and body aches. Objective: To...

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Main Authors:	Ferdosh, Sahena, Abdul Bari, Nurul Ashikin, Mia, Abdur Rashid, Sarker, Md. Zaidul Islam
Format:	Conference or Workshop Item
Language:	English English
Published:	2019
Subjects:	RS192 Materia Medica-Pharmaceutical Technology
Online Access:	http://irep.iium.edu.my/79158/ http://irep.iium.edu.my/79158/7/Poster.pdf http://irep.iium.edu.my/79158/8/aipc2019%20anisophyllea%20disticha.pdf

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spelling	iium-791582020-03-02T00:29:07Z http://irep.iium.edu.my/79158/ Optimization of bio-active compounds from stem of Anisophyllea disticha using supercritical fluid extraction technique Ferdosh, Sahena Abdul Bari, Nurul Ashikin Mia, Abdur Rashid Sarker, Md. Zaidul Islam RS192 Materia Medica-Pharmaceutical Technology Background: Anisophyllea disticha is belonging to the species of genus Anisophyllea, is not pharmacologically investigated despite its high utilization by the folklore in treating diarrhea, dysentery, jaundice, revitalizing the birth canal of women, relieving weariness and body aches. Objective: To optimize the total phenolic content (TPC) and antioxidant activities of supercritical fluid extracts of A. disticha in terms of DPPH radical scavenging activities and ferric reducing antioxidant power assay. Material and methods: Stem was chosen and subsequently extracted using supercritical fluid extraction (SFE) and further analyzed. Box-Behnken Design (BBD) was used to optimize and examine the effect of independent variables of SFE such as temperature (50-70˚C), pressure (20-30 MPa) and concentration of co-solvent (10-20%) on TPC and antioxidant activities of A. disticha. Results: The model suggested the optimal conditions of independent variables to be 50˚C, 20 MPa and 17% with predicted TPC of 53.35 mg GAE / g extract, scavenging activity of 96.61% and reducing power of 423.66 mg Fe (II) / g extract. Reverse-phase high performance liquid chromatography (HPLC) was developed for identification and quantification of phenolic compounds (gallic acid, p-coumaric, ferulic acid and quercetin) presented in A. disticha. Run 7 from SFE was chosen as it showed maximum TPC and potent activities and the responses obtained were compared with soxhlet Conclusion: The extract from SFE possessed remarkable TPC, antioxidant activities and concentration of phenolic compounds identified by HPLC, indicating its superior recovery of compounds. 2019-11-30 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/79158/7/Poster.pdf application/pdf en http://irep.iium.edu.my/79158/8/aipc2019%20anisophyllea%20disticha.pdf Ferdosh, Sahena and Abdul Bari, Nurul Ashikin and Mia, Abdur Rashid and Sarker, Md. Zaidul Islam (2019) Optimization of bio-active compounds from stem of Anisophyllea disticha using supercritical fluid extraction technique. In: AIMST International Pharmacy Conference (AIPC) 2019: Challenges and Opportunities in Pharmaceutical Sciences, Technology and Practice, 30 November - 1 December 2019, Faculty of Pharmacy, AIMST University, Semeling, Kedah. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	RS192 Materia Medica-Pharmaceutical Technology
spellingShingle	RS192 Materia Medica-Pharmaceutical Technology Ferdosh, Sahena Abdul Bari, Nurul Ashikin Mia, Abdur Rashid Sarker, Md. Zaidul Islam Optimization of bio-active compounds from stem of Anisophyllea disticha using supercritical fluid extraction technique
description	Background: Anisophyllea disticha is belonging to the species of genus Anisophyllea, is not pharmacologically investigated despite its high utilization by the folklore in treating diarrhea, dysentery, jaundice, revitalizing the birth canal of women, relieving weariness and body aches. Objective: To optimize the total phenolic content (TPC) and antioxidant activities of supercritical fluid extracts of A. disticha in terms of DPPH radical scavenging activities and ferric reducing antioxidant power assay. Material and methods: Stem was chosen and subsequently extracted using supercritical fluid extraction (SFE) and further analyzed. Box-Behnken Design (BBD) was used to optimize and examine the effect of independent variables of SFE such as temperature (50-70˚C), pressure (20-30 MPa) and concentration of co-solvent (10-20%) on TPC and antioxidant activities of A. disticha. Results: The model suggested the optimal conditions of independent variables to be 50˚C, 20 MPa and 17% with predicted TPC of 53.35 mg GAE / g extract, scavenging activity of 96.61% and reducing power of 423.66 mg Fe (II) / g extract. Reverse-phase high performance liquid chromatography (HPLC) was developed for identification and quantification of phenolic compounds (gallic acid, p-coumaric, ferulic acid and quercetin) presented in A. disticha. Run 7 from SFE was chosen as it showed maximum TPC and potent activities and the responses obtained were compared with soxhlet Conclusion: The extract from SFE possessed remarkable TPC, antioxidant activities and concentration of phenolic compounds identified by HPLC, indicating its superior recovery of compounds.
format	Conference or Workshop Item
author	Ferdosh, Sahena Abdul Bari, Nurul Ashikin Mia, Abdur Rashid Sarker, Md. Zaidul Islam
author_facet	Ferdosh, Sahena Abdul Bari, Nurul Ashikin Mia, Abdur Rashid Sarker, Md. Zaidul Islam
author_sort	Ferdosh, Sahena
title	Optimization of bio-active compounds from stem of Anisophyllea disticha using supercritical fluid extraction technique
title_short	Optimization of bio-active compounds from stem of Anisophyllea disticha using supercritical fluid extraction technique
title_full	Optimization of bio-active compounds from stem of Anisophyllea disticha using supercritical fluid extraction technique
title_fullStr	Optimization of bio-active compounds from stem of Anisophyllea disticha using supercritical fluid extraction technique
title_full_unstemmed	Optimization of bio-active compounds from stem of Anisophyllea disticha using supercritical fluid extraction technique
title_sort	optimization of bio-active compounds from stem of anisophyllea disticha using supercritical fluid extraction technique
publishDate	2019
url	http://irep.iium.edu.my/79158/ http://irep.iium.edu.my/79158/7/Poster.pdf http://irep.iium.edu.my/79158/8/aipc2019%20anisophyllea%20disticha.pdf
first_indexed	2023-09-18T21:51:18Z
last_indexed	2023-09-18T21:51:18Z
_version_	1777413787591114752

Optimization of bio-active compounds from stem of Anisophyllea disticha using supercritical fluid extraction technique

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