Comparative study of CDST & multiplex PCR to detect MBL producing Gram-negative bacilli among VAP patients admitted in a public medical college hospital of Bangladesh

Background: Ventilator-associated pneumonia (VAP) is the most common nosocomial infection in intensive care units (ICU), which accounts for 25% of all ICU infection. Documenting carbapenem-resistant gram-negative bacilli is very important as these strains may often cause outbreaks in the ICU setting...

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Main Authors:	Nusrat, Tanzina, Akter, Nasima, Haque, Mainul, A Rahman, Nor Azlina, Dewanjee, Arup Kanti, Ahmed, Shakeel, Rozario, Diana Thecla D
Format:	Article
Language:	English English
Published:	MDPI 2019
Subjects:	RZ Other systems of medicine
Online Access:	http://irep.iium.edu.my/79157/ http://irep.iium.edu.my/79157/ http://irep.iium.edu.my/79157/ http://irep.iium.edu.my/79157/1/2019-7%20Comparative%20Study%20Pathogens%20x.pdf http://irep.iium.edu.my/79157/7/79157_Comparative%20study%20of%20CDST%20%26%20multiplex%20PCR%20to%20detect%20MBL_SCOPUS.pdf

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spelling	iium-791572020-02-26T04:12:01Z http://irep.iium.edu.my/79157/ Comparative study of CDST & multiplex PCR to detect MBL producing Gram-negative bacilli among VAP patients admitted in a public medical college hospital of Bangladesh Nusrat, Tanzina Akter, Nasima Haque, Mainul A Rahman, Nor Azlina Dewanjee, Arup Kanti Ahmed, Shakeel Rozario, Diana Thecla D RZ Other systems of medicine Background: Ventilator-associated pneumonia (VAP) is the most common nosocomial infection in intensive care units (ICU), which accounts for 25% of all ICU infection. Documenting carbapenem-resistant gram-negative bacilli is very important as these strains may often cause outbreaks in the ICU setting and are responsible for the increased mortality and morbidity or limiting therapeutic options. The classical phenotypic method cannot provide an efficient means of diagnosis of the metallo-β-lactamases (MBLs) producer. Polymerase chain reaction (PCR) assays have lessened the importance of the phenotypic approach by detecting metallo-β-lactamase resistance genes such as New Delhi metallo-β-lactamase (NDM), Imipenemase (IMP), Verona integron-encoded metallo-β-lactamase (VIM), Sao Paulo metallo-β-lactamase (SPM), Germany Imipenemase (GIM). Objective: To compare the results of the Combined Disc Synergy Test (CDST) with that of the multiplex PCR to detect MBL-producing gram-negative bacilli. Materials and Method: A total of 105 endotracheal aspirates (ETA) samples were collected from the ICU of a public school in Bangladesh. This cross-sectional study was carried out in the Department of Microbiology, Chittagong for quantitative culture, CDST test, and multiplex PCR for blaIMP, blaVIM, blaNDM genes of MBL producers. Results: Among the 105 clinically suspected VAP cases, the quantitative culture was positive in 95 (90%) and among 95 g-negative bacilli isolated from VAP patients, 46 (48.42%) were imipenem resistant, 30 (65.22%) were MBL producers by CDST, 21 (45.65%) were identified as MBL producers by multiplex PCR. Conclusion: PCR was highly sensitive and specific for the detection of MBL producers. MDPI 2019-09-12 Article PeerReviewed application/pdf en http://irep.iium.edu.my/79157/1/2019-7%20Comparative%20Study%20Pathogens%20x.pdf application/pdf en http://irep.iium.edu.my/79157/7/79157_Comparative%20study%20of%20CDST%20%26%20multiplex%20PCR%20to%20detect%20MBL_SCOPUS.pdf Nusrat, Tanzina and Akter, Nasima and Haque, Mainul and A Rahman, Nor Azlina and Dewanjee, Arup Kanti and Ahmed, Shakeel and Rozario, Diana Thecla D (2019) Comparative study of CDST & multiplex PCR to detect MBL producing Gram-negative bacilli among VAP patients admitted in a public medical college hospital of Bangladesh. Pathogens, 8 (3). pp. 1-11. ISSN 2076-0817 https://www.mdpi.com/journal/pathogens 10.3390/pathogens8030151
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	RZ Other systems of medicine
spellingShingle	RZ Other systems of medicine Nusrat, Tanzina Akter, Nasima Haque, Mainul A Rahman, Nor Azlina Dewanjee, Arup Kanti Ahmed, Shakeel Rozario, Diana Thecla D Comparative study of CDST & multiplex PCR to detect MBL producing Gram-negative bacilli among VAP patients admitted in a public medical college hospital of Bangladesh
description	Background: Ventilator-associated pneumonia (VAP) is the most common nosocomial infection in intensive care units (ICU), which accounts for 25% of all ICU infection. Documenting carbapenem-resistant gram-negative bacilli is very important as these strains may often cause outbreaks in the ICU setting and are responsible for the increased mortality and morbidity or limiting therapeutic options. The classical phenotypic method cannot provide an efficient means of diagnosis of the metallo-β-lactamases (MBLs) producer. Polymerase chain reaction (PCR) assays have lessened the importance of the phenotypic approach by detecting metallo-β-lactamase resistance genes such as New Delhi metallo-β-lactamase (NDM), Imipenemase (IMP), Verona integron-encoded metallo-β-lactamase (VIM), Sao Paulo metallo-β-lactamase (SPM), Germany Imipenemase (GIM). Objective: To compare the results of the Combined Disc Synergy Test (CDST) with that of the multiplex PCR to detect MBL-producing gram-negative bacilli. Materials and Method: A total of 105 endotracheal aspirates (ETA) samples were collected from the ICU of a public school in Bangladesh. This cross-sectional study was carried out in the Department of Microbiology, Chittagong for quantitative culture, CDST test, and multiplex PCR for blaIMP, blaVIM, blaNDM genes of MBL producers. Results: Among the 105 clinically suspected VAP cases, the quantitative culture was positive in 95 (90%) and among 95 g-negative bacilli isolated from VAP patients, 46 (48.42%) were imipenem resistant, 30 (65.22%) were MBL producers by CDST, 21 (45.65%) were identified as MBL producers by multiplex PCR. Conclusion: PCR was highly sensitive and specific for the detection of MBL producers.
format	Article
author	Nusrat, Tanzina Akter, Nasima Haque, Mainul A Rahman, Nor Azlina Dewanjee, Arup Kanti Ahmed, Shakeel Rozario, Diana Thecla D
author_facet	Nusrat, Tanzina Akter, Nasima Haque, Mainul A Rahman, Nor Azlina Dewanjee, Arup Kanti Ahmed, Shakeel Rozario, Diana Thecla D
author_sort	Nusrat, Tanzina
title	Comparative study of CDST & multiplex PCR to detect MBL producing Gram-negative bacilli among VAP patients admitted in a public medical college hospital of Bangladesh
title_short	Comparative study of CDST & multiplex PCR to detect MBL producing Gram-negative bacilli among VAP patients admitted in a public medical college hospital of Bangladesh
title_full	Comparative study of CDST & multiplex PCR to detect MBL producing Gram-negative bacilli among VAP patients admitted in a public medical college hospital of Bangladesh
title_fullStr	Comparative study of CDST & multiplex PCR to detect MBL producing Gram-negative bacilli among VAP patients admitted in a public medical college hospital of Bangladesh
title_full_unstemmed	Comparative study of CDST & multiplex PCR to detect MBL producing Gram-negative bacilli among VAP patients admitted in a public medical college hospital of Bangladesh
title_sort	comparative study of cdst & multiplex pcr to detect mbl producing gram-negative bacilli among vap patients admitted in a public medical college hospital of bangladesh
publisher	MDPI
publishDate	2019
url	http://irep.iium.edu.my/79157/ http://irep.iium.edu.my/79157/ http://irep.iium.edu.my/79157/ http://irep.iium.edu.my/79157/1/2019-7%20Comparative%20Study%20Pathogens%20x.pdf http://irep.iium.edu.my/79157/7/79157_Comparative%20study%20of%20CDST%20%26%20multiplex%20PCR%20to%20detect%20MBL_SCOPUS.pdf
first_indexed	2023-09-18T21:51:18Z
last_indexed	2023-09-18T21:51:18Z
_version_	1777413787455848448

Comparative study of CDST & multiplex PCR to detect MBL producing Gram-negative bacilli among VAP patients admitted in a public medical college hospital of Bangladesh

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