Protein and toxin profiling at different growth phases of a. Tamiyavanichii and A.Leei

The naturally occurring phenomenon of harmful algae blooming (HAB) at the water column brought detrimental effects to the economy as well as the environmental health of the water ecosystem. Most cases of HABs reported in Malaysia waters are dominated by dinoflagellates Alexandrium spp. In this stud...

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Main Authors:	Muhamad Bunnori, Noraslinda, Abd Hamid, Shafida, Hamdan, Nurul Ashima, Abu Hassan, Muhamad Shafiq, Mohammad Noor, Normawaty
Format:	Conference or Workshop Item
Language:	English English English
Published:	Association for Computing Machinery 2019
Subjects:	QH301 Biology
Online Access:	http://irep.iium.edu.my/79070/ http://irep.iium.edu.my/79070/ http://irep.iium.edu.my/79070/ http://irep.iium.edu.my/79070/9/79070_Protein%20and%20Toxin%20Profiling%20at%20Different%20Growth_complete.pdf http://irep.iium.edu.my/79070/8/79070_Protein%20and%20Toxin%20Profiling%20at%20Different%20Growth_scopus.pdf http://irep.iium.edu.my/79070/20/79070_Protein%20and%20Toxin%20Profiling%20at%20Different%20Growth%20WOS.pdf

id	iium-79070
recordtype	eprints
spelling	iium-790702020-03-19T07:11:47Z http://irep.iium.edu.my/79070/ Protein and toxin profiling at different growth phases of a. Tamiyavanichii and A.Leei Muhamad Bunnori, Noraslinda Abd Hamid, Shafida Hamdan, Nurul Ashima Abu Hassan, Muhamad Shafiq Mohammad Noor, Normawaty QH301 Biology The naturally occurring phenomenon of harmful algae blooming (HAB) at the water column brought detrimental effects to the economy as well as the environmental health of the water ecosystem. Most cases of HABs reported in Malaysia waters are dominated by dinoflagellates Alexandrium spp. In this study, A. tamiyavanichii and A. leei with different toxicity levels were analyzed using two-dimensional PAGE and HPLC analysis. The growth pattern of both species was identified and compared by using proteomic approaches at each growth phases. Protein expression reduced throughout the growth phases of A. tamiyavanichii but elevated during stationary phase of A. leei. A short duration of stationary phase suggests the continuous expression of growth proteins in A. leei. GNAT family acetyltransferase and lipases were successfully identified enzyme protein in A. tamiyavanichii and A. leei respectively with growth regulatory functions. The toxin profiles of both species exhibited a higher level of toxin content in A. tamiyavanichii with 88 mol % of total toxins recorded as compared to 12 mol% in A. leei. The highest toxin content was recorded during the exponential phase of A. tamiyavanichii with a dominance of GTX4 and STX congeners. Fundamental studies of dinoflagellates from it’s molecular as well as byproduct analysis are useful to understand the biochemistry of the HAB species. The findings from this study can provide the basic knowledge on the biochemical properties of HAB species and the behavioral of affected organisms. Association for Computing Machinery 2019-03-16 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/79070/9/79070_Protein%20and%20Toxin%20Profiling%20at%20Different%20Growth_complete.pdf application/pdf en http://irep.iium.edu.my/79070/8/79070_Protein%20and%20Toxin%20Profiling%20at%20Different%20Growth_scopus.pdf application/pdf en http://irep.iium.edu.my/79070/20/79070_Protein%20and%20Toxin%20Profiling%20at%20Different%20Growth%20WOS.pdf Muhamad Bunnori, Noraslinda and Abd Hamid, Shafida and Hamdan, Nurul Ashima and Abu Hassan, Muhamad Shafiq and Mohammad Noor, Normawaty (2019) Protein and toxin profiling at different growth phases of a. Tamiyavanichii and A.Leei. In: "8th International Conference on Informatics, Environment, Energy and Applications, IEEA 2019", 16 - 19 March 2019, Osaka Japan. https://dl.acm.org/doi/proceedings/10.1145/3323716 10.1145/3323716.3323754
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English
topic	QH301 Biology
spellingShingle	QH301 Biology Muhamad Bunnori, Noraslinda Abd Hamid, Shafida Hamdan, Nurul Ashima Abu Hassan, Muhamad Shafiq Mohammad Noor, Normawaty Protein and toxin profiling at different growth phases of a. Tamiyavanichii and A.Leei
description	The naturally occurring phenomenon of harmful algae blooming (HAB) at the water column brought detrimental effects to the economy as well as the environmental health of the water ecosystem. Most cases of HABs reported in Malaysia waters are dominated by dinoflagellates Alexandrium spp. In this study, A. tamiyavanichii and A. leei with different toxicity levels were analyzed using two-dimensional PAGE and HPLC analysis. The growth pattern of both species was identified and compared by using proteomic approaches at each growth phases. Protein expression reduced throughout the growth phases of A. tamiyavanichii but elevated during stationary phase of A. leei. A short duration of stationary phase suggests the continuous expression of growth proteins in A. leei. GNAT family acetyltransferase and lipases were successfully identified enzyme protein in A. tamiyavanichii and A. leei respectively with growth regulatory functions. The toxin profiles of both species exhibited a higher level of toxin content in A. tamiyavanichii with 88 mol % of total toxins recorded as compared to 12 mol% in A. leei. The highest toxin content was recorded during the exponential phase of A. tamiyavanichii with a dominance of GTX4 and STX congeners. Fundamental studies of dinoflagellates from it’s molecular as well as byproduct analysis are useful to understand the biochemistry of the HAB species. The findings from this study can provide the basic knowledge on the biochemical properties of HAB species and the behavioral of affected organisms.
format	Conference or Workshop Item
author	Muhamad Bunnori, Noraslinda Abd Hamid, Shafida Hamdan, Nurul Ashima Abu Hassan, Muhamad Shafiq Mohammad Noor, Normawaty
author_facet	Muhamad Bunnori, Noraslinda Abd Hamid, Shafida Hamdan, Nurul Ashima Abu Hassan, Muhamad Shafiq Mohammad Noor, Normawaty
author_sort	Muhamad Bunnori, Noraslinda
title	Protein and toxin profiling at different growth phases of a. Tamiyavanichii and A.Leei
title_short	Protein and toxin profiling at different growth phases of a. Tamiyavanichii and A.Leei
title_full	Protein and toxin profiling at different growth phases of a. Tamiyavanichii and A.Leei
title_fullStr	Protein and toxin profiling at different growth phases of a. Tamiyavanichii and A.Leei
title_full_unstemmed	Protein and toxin profiling at different growth phases of a. Tamiyavanichii and A.Leei
title_sort	protein and toxin profiling at different growth phases of a. tamiyavanichii and a.leei
publisher	Association for Computing Machinery
publishDate	2019
url	http://irep.iium.edu.my/79070/ http://irep.iium.edu.my/79070/ http://irep.iium.edu.my/79070/ http://irep.iium.edu.my/79070/9/79070_Protein%20and%20Toxin%20Profiling%20at%20Different%20Growth_complete.pdf http://irep.iium.edu.my/79070/8/79070_Protein%20and%20Toxin%20Profiling%20at%20Different%20Growth_scopus.pdf http://irep.iium.edu.my/79070/20/79070_Protein%20and%20Toxin%20Profiling%20at%20Different%20Growth%20WOS.pdf
first_indexed	2023-09-18T21:51:14Z
last_indexed	2023-09-18T21:51:14Z
_version_	1777413782921805824

Protein and toxin profiling at different growth phases of a. Tamiyavanichii and A.Leei

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