Antibacterial activity of ethanol extract of Sargassum polycystum against Streptococcus mutans and Lactobacillus casei: in vitro

Introduction: Sargassum polycystum (brown seaweeds) has been recognized as a potential source of natural antibacterial and antimicrobial compounds. It has been used in many applications and products as they possess interesting biological activities that give benefit to the dental and medical area. A...

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Main Authors:	Ab. Halim, Noorhazayti, Zakaria, Nurul Syahidah, Md Hisham, Sarah Shafinaz, Husain, Juzaily, Md Mustafa, Md Muziman Syah
Format:	Article
Language:	English
Published:	2020
Subjects:	RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/79022/ http://irep.iium.edu.my/79022/1/Noorhazayti%20et%20al.%202020.%20ANTIBACTERIAL%20ACTIVITY%20OF%20ETHANOL%20EXTRACT%20OF%20SARGASSUM%20POLYCYSTUM%20AGAINST%20STREPTOCOCCUS%20MUTANS%20AND%20LACTOBACILLUS%20CASEI-%20IN%20VITRO.pdf

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spelling	iium-790222020-02-26T15:00:28Z http://irep.iium.edu.my/79022/ Antibacterial activity of ethanol extract of Sargassum polycystum against Streptococcus mutans and Lactobacillus casei: in vitro Ab. Halim, Noorhazayti Zakaria, Nurul Syahidah Md Hisham, Sarah Shafinaz Husain, Juzaily Md Mustafa, Md Muziman Syah RS Pharmacy and materia medica Introduction: Sargassum polycystum (brown seaweeds) has been recognized as a potential source of natural antibacterial and antimicrobial compounds. It has been used in many applications and products as they possess interesting biological activities that give benefit to the dental and medical area. Aims: The objectives of this study were to determine the growth curve of Streptococcus mutans and Lactobacillus casei, and also to determine minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) value of Sargassum polycystum extract against Streptococcus mutans and Lactobacillus casei. Materials and methods: The growth of both bacteria were studied by growth curve analysis. Besides that, the antibacterial activity of Sargassum polycystum ethanol extracts against two oral cariogenic bacteria were examined using broth dilution method. The bioactivity of the seaweed extract was studied by finding the MIC and MBC value. Results: The time taken for the Streptococcus mutans and Lactobacillus casei to achieve exponential phase during growth is 4 and 5 hours respectively. MIC values of ethanol extracts of Sargassum polycystum in different concentration against Streptococcus mutans and Lactobacillus casei were unreliable because the presence of fungus (white-precipitate) at all 96-well plates including the positive and negative control. As there was no MIC value, partial inhibition was determined by calculating the optical density. Therefore, MBC analysis was unable to carry out because the MIC values were unreliable. Conclusion: The ethanol extract of Sargassum polycystum used in this study show partial bacterial inhibition against pathogens used. Therefore, the same study by using different type of solvent are recommended to be carried out in the future. 2020 Article PeerReviewed application/pdf en http://irep.iium.edu.my/79022/1/Noorhazayti%20et%20al.%202020.%20ANTIBACTERIAL%20ACTIVITY%20OF%20ETHANOL%20EXTRACT%20OF%20SARGASSUM%20POLYCYSTUM%20AGAINST%20STREPTOCOCCUS%20MUTANS%20AND%20LACTOBACILLUS%20CASEI-%20IN%20VITRO.pdf Ab. Halim, Noorhazayti and Zakaria, Nurul Syahidah and Md Hisham, Sarah Shafinaz and Husain, Juzaily and Md Mustafa, Md Muziman Syah (2020) Antibacterial activity of ethanol extract of Sargassum polycystum against Streptococcus mutans and Lactobacillus casei: in vitro. International Journal of Allied Health Sciences, 4 (1). pp. 1121-1127. E-ISSN 2600-8491
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RS Pharmacy and materia medica
spellingShingle	RS Pharmacy and materia medica Ab. Halim, Noorhazayti Zakaria, Nurul Syahidah Md Hisham, Sarah Shafinaz Husain, Juzaily Md Mustafa, Md Muziman Syah Antibacterial activity of ethanol extract of Sargassum polycystum against Streptococcus mutans and Lactobacillus casei: in vitro
description	Introduction: Sargassum polycystum (brown seaweeds) has been recognized as a potential source of natural antibacterial and antimicrobial compounds. It has been used in many applications and products as they possess interesting biological activities that give benefit to the dental and medical area. Aims: The objectives of this study were to determine the growth curve of Streptococcus mutans and Lactobacillus casei, and also to determine minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) value of Sargassum polycystum extract against Streptococcus mutans and Lactobacillus casei. Materials and methods: The growth of both bacteria were studied by growth curve analysis. Besides that, the antibacterial activity of Sargassum polycystum ethanol extracts against two oral cariogenic bacteria were examined using broth dilution method. The bioactivity of the seaweed extract was studied by finding the MIC and MBC value. Results: The time taken for the Streptococcus mutans and Lactobacillus casei to achieve exponential phase during growth is 4 and 5 hours respectively. MIC values of ethanol extracts of Sargassum polycystum in different concentration against Streptococcus mutans and Lactobacillus casei were unreliable because the presence of fungus (white-precipitate) at all 96-well plates including the positive and negative control. As there was no MIC value, partial inhibition was determined by calculating the optical density. Therefore, MBC analysis was unable to carry out because the MIC values were unreliable. Conclusion: The ethanol extract of Sargassum polycystum used in this study show partial bacterial inhibition against pathogens used. Therefore, the same study by using different type of solvent are recommended to be carried out in the future.
format	Article
author	Ab. Halim, Noorhazayti Zakaria, Nurul Syahidah Md Hisham, Sarah Shafinaz Husain, Juzaily Md Mustafa, Md Muziman Syah
author_facet	Ab. Halim, Noorhazayti Zakaria, Nurul Syahidah Md Hisham, Sarah Shafinaz Husain, Juzaily Md Mustafa, Md Muziman Syah
author_sort	Ab. Halim, Noorhazayti
title	Antibacterial activity of ethanol extract of Sargassum polycystum against Streptococcus mutans and Lactobacillus casei: in vitro
title_short	Antibacterial activity of ethanol extract of Sargassum polycystum against Streptococcus mutans and Lactobacillus casei: in vitro
title_full	Antibacterial activity of ethanol extract of Sargassum polycystum against Streptococcus mutans and Lactobacillus casei: in vitro
title_fullStr	Antibacterial activity of ethanol extract of Sargassum polycystum against Streptococcus mutans and Lactobacillus casei: in vitro
title_full_unstemmed	Antibacterial activity of ethanol extract of Sargassum polycystum against Streptococcus mutans and Lactobacillus casei: in vitro
title_sort	antibacterial activity of ethanol extract of sargassum polycystum against streptococcus mutans and lactobacillus casei: in vitro
publishDate	2020
url	http://irep.iium.edu.my/79022/ http://irep.iium.edu.my/79022/1/Noorhazayti%20et%20al.%202020.%20ANTIBACTERIAL%20ACTIVITY%20OF%20ETHANOL%20EXTRACT%20OF%20SARGASSUM%20POLYCYSTUM%20AGAINST%20STREPTOCOCCUS%20MUTANS%20AND%20LACTOBACILLUS%20CASEI-%20IN%20VITRO.pdf
first_indexed	2023-09-18T21:51:11Z
last_indexed	2023-09-18T21:51:11Z
_version_	1777413779506593792

Antibacterial activity of ethanol extract of Sargassum polycystum against Streptococcus mutans and Lactobacillus casei: in vitro

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