Understanding the effects of different parameters of Soxhlet extraction on bioactive compounds from Aquilaria malaccensis leaf through GCMS-based profiling

Bioactive compounds from plants have been shown to possess therapeutic values which are being used as medicine as well as ingredients for functional foods and nutraceuticals. While qualitative and quantitative studies on the bioactive compounds of plant extract are important to establish their cl...

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Main Authors:	Zainurin, Nur Aimi Aliah, Hashim, Yumi Zuhanis Has-Yun, Mohd Azmin, Nor Fadhillah, Al-Khatib, Ma An Fahmi Rashid
Format:	Article
Language:	English
Published:	Food Research 2020
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/78772/ http://irep.iium.edu.my/78772/ http://irep.iium.edu.my/78772/ http://irep.iium.edu.my/78772/1/Paper_Zainurin%20et%20al%202020%20%28Food%20Res%29.pdf

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spelling	iium-787722020-03-16T02:13:27Z http://irep.iium.edu.my/78772/ Understanding the effects of different parameters of Soxhlet extraction on bioactive compounds from Aquilaria malaccensis leaf through GCMS-based profiling Zainurin, Nur Aimi Aliah Hashim, Yumi Zuhanis Has-Yun Mohd Azmin, Nor Fadhillah Al-Khatib, Ma An Fahmi Rashid Q Science (General) Bioactive compounds from plants have been shown to possess therapeutic values which are being used as medicine as well as ingredients for functional foods and nutraceuticals. While qualitative and quantitative studies on the bioactive compounds of plant extract are important to establish their claimed therapeutic properties; less is focused on effects and interrelationship of process (extraction) conditions used to obtain the desired bioactive compounds. As such, this study intended to determine the effects of different parameters on the extraction of Aquilaria malaccensis leaf extract (ALEX) bioactive compounds. Metabolite profiling of bioactive compounds of ALEX samples was conducted using gas chromatography-mass spectrometry (GCMS) with subsequent multivariate statistical analysis through principal component analysis (PCA) with the aid of MATLAB software. The GCMS analysis detected diverse potential bioactive compounds in ALEX samples with hydrocarbon, terpenes and esters being the primary groups. n-hexadecanoic acid and hexadecanoic acid, 2-hydroxy-1-(hydroxymethyl)ethyl ester were found to be present in most of the ALEX samples. PCA showed that same Soxhlet extraction parameters were able to cluster the samples together and separate samples extracted with different extraction parameters based on the relative abundance of the particular compounds. In conclusion, it is notable that the samples were significantly separated based on Soxhlet extraction time as compared to the solid to solvent ratio and the suggested best Soxhlet extraction parameters were 18 hrs and 1:70 solid to solvent ratio. The findings from this study will facilitate the extraction and purification of the desired bioactive compounds from agarwood leaf that can be further used in development of functional foods and nutraceuticals Food Research 2020 Article PeerReviewed application/pdf en http://irep.iium.edu.my/78772/1/Paper_Zainurin%20et%20al%202020%20%28Food%20Res%29.pdf Zainurin, Nur Aimi Aliah and Hashim, Yumi Zuhanis Has-Yun and Mohd Azmin, Nor Fadhillah and Al-Khatib, Ma An Fahmi Rashid (2020) Understanding the effects of different parameters of Soxhlet extraction on bioactive compounds from Aquilaria malaccensis leaf through GCMS-based profiling. Food Research, 4 (Supplement 1). 63 -73. ISSN 2550-2166 https://www.myfoodresearch.com/uploads/8/4/8/5/84855864/_7__fr-2019-s09.r1_zainurin.pdf 10.26656/fr.2017.4(S1).S09
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Zainurin, Nur Aimi Aliah Hashim, Yumi Zuhanis Has-Yun Mohd Azmin, Nor Fadhillah Al-Khatib, Ma An Fahmi Rashid Understanding the effects of different parameters of Soxhlet extraction on bioactive compounds from Aquilaria malaccensis leaf through GCMS-based profiling
description	Bioactive compounds from plants have been shown to possess therapeutic values which are being used as medicine as well as ingredients for functional foods and nutraceuticals. While qualitative and quantitative studies on the bioactive compounds of plant extract are important to establish their claimed therapeutic properties; less is focused on effects and interrelationship of process (extraction) conditions used to obtain the desired bioactive compounds. As such, this study intended to determine the effects of different parameters on the extraction of Aquilaria malaccensis leaf extract (ALEX) bioactive compounds. Metabolite profiling of bioactive compounds of ALEX samples was conducted using gas chromatography-mass spectrometry (GCMS) with subsequent multivariate statistical analysis through principal component analysis (PCA) with the aid of MATLAB software. The GCMS analysis detected diverse potential bioactive compounds in ALEX samples with hydrocarbon, terpenes and esters being the primary groups. n-hexadecanoic acid and hexadecanoic acid, 2-hydroxy-1-(hydroxymethyl)ethyl ester were found to be present in most of the ALEX samples. PCA showed that same Soxhlet extraction parameters were able to cluster the samples together and separate samples extracted with different extraction parameters based on the relative abundance of the particular compounds. In conclusion, it is notable that the samples were significantly separated based on Soxhlet extraction time as compared to the solid to solvent ratio and the suggested best Soxhlet extraction parameters were 18 hrs and 1:70 solid to solvent ratio. The findings from this study will facilitate the extraction and purification of the desired bioactive compounds from agarwood leaf that can be further used in development of functional foods and nutraceuticals
format	Article
author	Zainurin, Nur Aimi Aliah Hashim, Yumi Zuhanis Has-Yun Mohd Azmin, Nor Fadhillah Al-Khatib, Ma An Fahmi Rashid
author_facet	Zainurin, Nur Aimi Aliah Hashim, Yumi Zuhanis Has-Yun Mohd Azmin, Nor Fadhillah Al-Khatib, Ma An Fahmi Rashid
author_sort	Zainurin, Nur Aimi Aliah
title	Understanding the effects of different parameters of Soxhlet extraction on bioactive compounds from Aquilaria malaccensis leaf through GCMS-based profiling
title_short	Understanding the effects of different parameters of Soxhlet extraction on bioactive compounds from Aquilaria malaccensis leaf through GCMS-based profiling
title_full	Understanding the effects of different parameters of Soxhlet extraction on bioactive compounds from Aquilaria malaccensis leaf through GCMS-based profiling
title_fullStr	Understanding the effects of different parameters of Soxhlet extraction on bioactive compounds from Aquilaria malaccensis leaf through GCMS-based profiling
title_full_unstemmed	Understanding the effects of different parameters of Soxhlet extraction on bioactive compounds from Aquilaria malaccensis leaf through GCMS-based profiling
title_sort	understanding the effects of different parameters of soxhlet extraction on bioactive compounds from aquilaria malaccensis leaf through gcms-based profiling
publisher	Food Research
publishDate	2020
url	http://irep.iium.edu.my/78772/ http://irep.iium.edu.my/78772/ http://irep.iium.edu.my/78772/ http://irep.iium.edu.my/78772/1/Paper_Zainurin%20et%20al%202020%20%28Food%20Res%29.pdf
first_indexed	2023-09-18T21:50:54Z
last_indexed	2023-09-18T21:50:54Z
_version_	1777413761751056384

Understanding the effects of different parameters of Soxhlet extraction on bioactive compounds from Aquilaria malaccensis leaf through GCMS-based profiling

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