A new method for determining aflatoxins in groundnut and groundnut cake using FTIR spectroscopy with attenuated total reflectance technique

A new analytical method was developed for the determination of aflatoxins in groundnut and groundnut cakes by Fourier transform infrared (FTIR) spectroscopy using horizontal attenuated total reflectance technique. Groundnut and groundnut cake samples were used in this study. The wavelengths were s...

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Main Authors:	Mirghani, Mohamed Elwathig Saeed, Che Man, Yakoob, Jinap , Selamat, Baharain , B. S., Bakar, Jamilah
Format:	Article
Language:	English
Published:	Springer Berlin 2001
Subjects:	T Technology (General)
Online Access:	http://irep.iium.edu.my/7869/ http://irep.iium.edu.my/7869/ http://irep.iium.edu.my/7869/ http://irep.iium.edu.my/7869/1/Elwathig_%28Aflatoxin%29.pdf

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spelling	iium-78692012-05-07T06:17:44Z http://irep.iium.edu.my/7869/ A new method for determining aflatoxins in groundnut and groundnut cake using FTIR spectroscopy with attenuated total reflectance technique Mirghani, Mohamed Elwathig Saeed Che Man, Yakoob Jinap , Selamat Baharain , B. S. Bakar, Jamilah T Technology (General) A new analytical method was developed for the determination of aflatoxins in groundnut and groundnut cakes by Fourier transform infrared (FTIR) spectroscopy using horizontal attenuated total reflectance technique. Groundnut and groundnut cake samples were used in this study. The wavelengths were selected for the four types of aflatoxins—B1, B2, G1, and G2—and the standards prepared for each by spiking some clean sample with the aflatoxins in concentrations of 0–1200 parts per billion. A partial least square regression was used to derive the calibration models for each toxin. The coefficients of determination (R2) of the calibration model were computed for the FTIR spectroscopy predicted values vs. actual values of aflatoxins in parts per billion. The R2 was found to be 0.9911, 0.9859, 0.9986, and 0.9789 for aflatoxins B1, B2, G1, and G2, respectively. Standard errors of calibration for groundnut samples were found to be 1.80, 2.03, 1.42, and 2.05 for aflatoxins B1, B2, G1, and G2, respectively. Calibration models were validated with an independent set of samples. The R2 of validation models were computed. The SD of the difference for repeatability of the FTIR method was found to be better than that of the chemical method. Based on the results obtained, FTIR spectroscopy can be a useful instrumental method for determining aflatoxins in oilseeds and oilseed cakes. With its speed and ease of data manipulation by computer software, it is a possible alternative to the standard wet chemical methods for a rapid and accurate routine determination of aflatoxin levels in food and feed. Springer Berlin 2001 Article PeerReviewed application/pdf en http://irep.iium.edu.my/7869/1/Elwathig_%28Aflatoxin%29.pdf Mirghani, Mohamed Elwathig Saeed and Che Man, Yakoob and Jinap , Selamat and Baharain , B. S. and Bakar, Jamilah (2001) A new method for determining aflatoxins in groundnut and groundnut cake using FTIR spectroscopy with attenuated total reflectance technique. Journal Of The American Oil Chemists' Society, 78 (10). pp. 985-992. ISSN 0003-021X http://www.aocs.org/Journals/jaocs.cfm Paper no. J9825 in JAOCS 78, 985–992 (October 2001).
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	T Technology (General)
spellingShingle	T Technology (General) Mirghani, Mohamed Elwathig Saeed Che Man, Yakoob Jinap , Selamat Baharain , B. S. Bakar, Jamilah A new method for determining aflatoxins in groundnut and groundnut cake using FTIR spectroscopy with attenuated total reflectance technique
description	A new analytical method was developed for the determination of aflatoxins in groundnut and groundnut cakes by Fourier transform infrared (FTIR) spectroscopy using horizontal attenuated total reflectance technique. Groundnut and groundnut cake samples were used in this study. The wavelengths were selected for the four types of aflatoxins—B1, B2, G1, and G2—and the standards prepared for each by spiking some clean sample with the aflatoxins in concentrations of 0–1200 parts per billion. A partial least square regression was used to derive the calibration models for each toxin. The coefficients of determination (R2) of the calibration model were computed for the FTIR spectroscopy predicted values vs. actual values of aflatoxins in parts per billion. The R2 was found to be 0.9911, 0.9859, 0.9986, and 0.9789 for aflatoxins B1, B2, G1, and G2, respectively. Standard errors of calibration for groundnut samples were found to be 1.80, 2.03, 1.42, and 2.05 for aflatoxins B1, B2, G1, and G2, respectively. Calibration models were validated with an independent set of samples. The R2 of validation models were computed. The SD of the difference for repeatability of the FTIR method was found to be better than that of the chemical method. Based on the results obtained, FTIR spectroscopy can be a useful instrumental method for determining aflatoxins in oilseeds and oilseed cakes. With its speed and ease of data manipulation by computer software, it is a possible alternative to the standard wet chemical methods for a rapid and accurate routine determination of aflatoxin levels in food and feed.
format	Article
author	Mirghani, Mohamed Elwathig Saeed Che Man, Yakoob Jinap , Selamat Baharain , B. S. Bakar, Jamilah
author_facet	Mirghani, Mohamed Elwathig Saeed Che Man, Yakoob Jinap , Selamat Baharain , B. S. Bakar, Jamilah
author_sort	Mirghani, Mohamed Elwathig Saeed
title	A new method for determining aflatoxins in groundnut and groundnut cake using FTIR spectroscopy with attenuated total reflectance technique
title_short	A new method for determining aflatoxins in groundnut and groundnut cake using FTIR spectroscopy with attenuated total reflectance technique
title_full	A new method for determining aflatoxins in groundnut and groundnut cake using FTIR spectroscopy with attenuated total reflectance technique
title_fullStr	A new method for determining aflatoxins in groundnut and groundnut cake using FTIR spectroscopy with attenuated total reflectance technique
title_full_unstemmed	A new method for determining aflatoxins in groundnut and groundnut cake using FTIR spectroscopy with attenuated total reflectance technique
title_sort	new method for determining aflatoxins in groundnut and groundnut cake using ftir spectroscopy with attenuated total reflectance technique
publisher	Springer Berlin
publishDate	2001
url	http://irep.iium.edu.my/7869/ http://irep.iium.edu.my/7869/ http://irep.iium.edu.my/7869/ http://irep.iium.edu.my/7869/1/Elwathig_%28Aflatoxin%29.pdf
first_indexed	2023-09-18T20:17:25Z
last_indexed	2023-09-18T20:17:25Z
_version_	1777407880776908800

A new method for determining aflatoxins in groundnut and groundnut cake using FTIR spectroscopy with attenuated total reflectance technique

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