Metabolite profiling of Aquilaria malaccensis leaf extract using liquid chromatography-Q-TOF-mass spectrometry and investigation of its potential antilipoxygenase activity in vitro.

The Aquilaria malaccensis species of the genus Aquilaria is an abundant source of agarwood resin and many bioactive phytochemicals. Recent data regarding the chemical constituents and biological activities of Aquilaria leaves led us to attempt to qualitatively profile the metabolites of Aquilaria...

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Main Authors:	Eissa, Manar, Hashim, Yumi Zuhanis Has-Yun, El-Kersh, Dina, Syed Abd Azizz, Saripah Salbiah, Mohd Salleh, Hamzah, Md Isa, Muhammad Lokman, Abd Warif, Nor Malia
Format:	Article
Language:	English
Published:	MDPI 2020
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/78529/ http://irep.iium.edu.my/78529/ http://irep.iium.edu.my/78529/ http://irep.iium.edu.my/78529/1/Paper_Manar%20et%20al%202020%20%28Processes%29.pdf

id	iium-78529
recordtype	eprints
spelling	iium-785292020-03-04T07:02:26Z http://irep.iium.edu.my/78529/ Metabolite profiling of Aquilaria malaccensis leaf extract using liquid chromatography-Q-TOF-mass spectrometry and investigation of its potential antilipoxygenase activity in vitro. Eissa, Manar Hashim, Yumi Zuhanis Has-Yun El-Kersh, Dina Syed Abd Azizz, Saripah Salbiah Mohd Salleh, Hamzah Md Isa, Muhammad Lokman Abd Warif, Nor Malia Q Science (General) The Aquilaria malaccensis species of the genus Aquilaria is an abundant source of agarwood resin and many bioactive phytochemicals. Recent data regarding the chemical constituents and biological activities of Aquilaria leaves led us to attempt to qualitatively profile the metabolites of Aquilaria malaccensis leaves from a healthy, noninoculated tree through phytochemical screening, GC‐MS, and LC/Q‐TOF‐MS. The present work is also the first to report the antilipoxygenase activity of A. malaccensis leaves from healthy noninoculated tree and investigate its toxicity on oral mucosal cells. A total of 53 compounds were tentatively identified in the extract, some of which have been described in literature as exhibiting anti‐inflammatory activity. A number of novel compounds were identified for the first time, including quercetin, quercetin‐O‐hexoside, kaempferol‐O‐ dirhamnoside, isorhamnetin‐O‐hexoside, syringetin‐O‐hexoside, myricetin, tetrahydroxyflavanone, hesperetin, sissotrin, and lupeol. The antilipoxygenase assay was used to determine the lipoxygenase (LOX) inhibitory potential of the extract, while a WST‐1 assay was conducted to investigate the effect of the extract on oral epithelial cells (OEC). The extract implied moderate anti‐LOX activity with IC50 value of 71.6 μg/mL. Meanwhile, the cell viability of OEC ranged between 92.55% (10 μg/mL)–76.06% ± (100 μg/mL) upon treatment, indicating some potential toxicity risks. The results attained encourage future studies of the isolation of bioactive compounds from Aquilaria malaccensis leaves, as well as further investigation on the anti‐ inflammatory mechanisms and toxicity associated with their use. MDPI 2020-02 Article PeerReviewed application/pdf en http://irep.iium.edu.my/78529/1/Paper_Manar%20et%20al%202020%20%28Processes%29.pdf Eissa, Manar and Hashim, Yumi Zuhanis Has-Yun and El-Kersh, Dina and Syed Abd Azizz, Saripah Salbiah and Mohd Salleh, Hamzah and Md Isa, Muhammad Lokman and Abd Warif, Nor Malia (2020) Metabolite profiling of Aquilaria malaccensis leaf extract using liquid chromatography-Q-TOF-mass spectrometry and investigation of its potential antilipoxygenase activity in vitro. Processes, 8 (2). pp. 1-22. E-ISSN 2227-9717 https://www.mdpi.com/2227-9717/8/2/202/htm 10.3390/pr8020202
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Eissa, Manar Hashim, Yumi Zuhanis Has-Yun El-Kersh, Dina Syed Abd Azizz, Saripah Salbiah Mohd Salleh, Hamzah Md Isa, Muhammad Lokman Abd Warif, Nor Malia Metabolite profiling of Aquilaria malaccensis leaf extract using liquid chromatography-Q-TOF-mass spectrometry and investigation of its potential antilipoxygenase activity in vitro.
description	The Aquilaria malaccensis species of the genus Aquilaria is an abundant source of agarwood resin and many bioactive phytochemicals. Recent data regarding the chemical constituents and biological activities of Aquilaria leaves led us to attempt to qualitatively profile the metabolites of Aquilaria malaccensis leaves from a healthy, noninoculated tree through phytochemical screening, GC‐MS, and LC/Q‐TOF‐MS. The present work is also the first to report the antilipoxygenase activity of A. malaccensis leaves from healthy noninoculated tree and investigate its toxicity on oral mucosal cells. A total of 53 compounds were tentatively identified in the extract, some of which have been described in literature as exhibiting anti‐inflammatory activity. A number of novel compounds were identified for the first time, including quercetin, quercetin‐O‐hexoside, kaempferol‐O‐ dirhamnoside, isorhamnetin‐O‐hexoside, syringetin‐O‐hexoside, myricetin, tetrahydroxyflavanone, hesperetin, sissotrin, and lupeol. The antilipoxygenase assay was used to determine the lipoxygenase (LOX) inhibitory potential of the extract, while a WST‐1 assay was conducted to investigate the effect of the extract on oral epithelial cells (OEC). The extract implied moderate anti‐LOX activity with IC50 value of 71.6 μg/mL. Meanwhile, the cell viability of OEC ranged between 92.55% (10 μg/mL)–76.06% ± (100 μg/mL) upon treatment, indicating some potential toxicity risks. The results attained encourage future studies of the isolation of bioactive compounds from Aquilaria malaccensis leaves, as well as further investigation on the anti‐ inflammatory mechanisms and toxicity associated with their use.
format	Article
author	Eissa, Manar Hashim, Yumi Zuhanis Has-Yun El-Kersh, Dina Syed Abd Azizz, Saripah Salbiah Mohd Salleh, Hamzah Md Isa, Muhammad Lokman Abd Warif, Nor Malia
author_facet	Eissa, Manar Hashim, Yumi Zuhanis Has-Yun El-Kersh, Dina Syed Abd Azizz, Saripah Salbiah Mohd Salleh, Hamzah Md Isa, Muhammad Lokman Abd Warif, Nor Malia
author_sort	Eissa, Manar
title	Metabolite profiling of Aquilaria malaccensis leaf extract using liquid chromatography-Q-TOF-mass spectrometry and investigation of its potential antilipoxygenase activity in vitro.
title_short	Metabolite profiling of Aquilaria malaccensis leaf extract using liquid chromatography-Q-TOF-mass spectrometry and investigation of its potential antilipoxygenase activity in vitro.
title_full	Metabolite profiling of Aquilaria malaccensis leaf extract using liquid chromatography-Q-TOF-mass spectrometry and investigation of its potential antilipoxygenase activity in vitro.
title_fullStr	Metabolite profiling of Aquilaria malaccensis leaf extract using liquid chromatography-Q-TOF-mass spectrometry and investigation of its potential antilipoxygenase activity in vitro.
title_full_unstemmed	Metabolite profiling of Aquilaria malaccensis leaf extract using liquid chromatography-Q-TOF-mass spectrometry and investigation of its potential antilipoxygenase activity in vitro.
title_sort	metabolite profiling of aquilaria malaccensis leaf extract using liquid chromatography-q-tof-mass spectrometry and investigation of its potential antilipoxygenase activity in vitro.
publisher	MDPI
publishDate	2020
url	http://irep.iium.edu.my/78529/ http://irep.iium.edu.my/78529/ http://irep.iium.edu.my/78529/ http://irep.iium.edu.my/78529/1/Paper_Manar%20et%20al%202020%20%28Processes%29.pdf
first_indexed	2023-09-18T21:50:38Z
last_indexed	2023-09-18T21:50:38Z
_version_	1777413744935043072

Metabolite profiling of Aquilaria malaccensis leaf extract using liquid chromatography-Q-TOF-mass spectrometry and investigation of its potential antilipoxygenase activity in vitro.

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