Qualitative and Quantitative Phenolic Compounds Analysis of Dicranopteris linearis Different Fractional Polarities Leaves Extract

Dicranopteris linearis occupies in an open ground that contains poor soils and often colonizing disturbed space that partly shaded area. It has been known for various traditional values including medicinal, edible food, soil erosion protection, pen and furniture. Even though the plants possess bot...

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Main Authors: Othman, Rashidi, Ramya, Razanah, Mohd Hassan, Norazian, Kammona, Suhair
Format: Article
Language:English
Published: SET PUBLISHER 2020
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Online Access:http://irep.iium.edu.my/78491/
http://irep.iium.edu.my/78491/1/JPANSV10N1A2-Othman.pdf
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spelling iium-784912020-02-25T08:19:37Z http://irep.iium.edu.my/78491/ Qualitative and Quantitative Phenolic Compounds Analysis of Dicranopteris linearis Different Fractional Polarities Leaves Extract Othman, Rashidi Ramya, Razanah Mohd Hassan, Norazian Kammona, Suhair GE Environmental Sciences RS Pharmacy and materia medica SB Plant culture TP248.13 Biotechnology TP934 Paints, pigments, varnishes, etc Dicranopteris linearis occupies in an open ground that contains poor soils and often colonizing disturbed space that partly shaded area. It has been known for various traditional values including medicinal, edible food, soil erosion protection, pen and furniture. Even though the plants possess both economic and medicinal value, they still form the neglected group of a plant. The present study was carried out to characterize the phenolic compounds in D. linearis leaves extract in different fractional polarities qualitative and quantitatively. Dried leaves of D. linearis were successfully extracted by using water extraction before separated by petroleum ether, ethyl acetate and butanol fractions. All the fractional extracts have been analysed by using GCTOF-MS and HPLC. The result from GCTOF-MS analysis of fractional extracts showed 38 compounds found in petroleum ether, ethyl acetate and butanol extracts. However, only four phenolic compounds were identified through HPLC analysis in ethyl acetate and butanol extracts which were 2-Methoxy-4-vinylphenol, Vanillin, 4-Hydroxybenzaldehyde and 4-Hydroxybenzohydrazide. The results revealed that D. linearis contained 699.83 ± 6.26 μg GAE /g DW of total phenolic acid whereas individual phenolic acids were predominantly caffeic acid (0.44 ± 0.01 μg/g DW) and ferulic acid (0.22 ± 0.00 μg/g DW) in ethyl acetate and caffeic acid (0.10 ± 0.00 μg/g DW) and 2-Coumaric acid (0.44 ± 0.00 μg/g DW) in butanol extracts. In the present study, the plant extracts demonstrated the highest phenolic compound detected in ethyl acetate and butanol compared to petroleum ether extract. SET PUBLISHER 2020-02-04 Article PeerReviewed application/pdf en http://irep.iium.edu.my/78491/1/JPANSV10N1A2-Othman.pdf Othman, Rashidi and Ramya, Razanah and Mohd Hassan, Norazian and Kammona, Suhair (2020) Qualitative and Quantitative Phenolic Compounds Analysis of Dicranopteris linearis Different Fractional Polarities Leaves Extract. Journal of Pharmacy and Nutrition Sciences, 10 (1). pp. 7-12. ISSN 2223-3806 E-ISSN 1927-5951
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic GE Environmental Sciences
RS Pharmacy and materia medica
SB Plant culture
TP248.13 Biotechnology
TP934 Paints, pigments, varnishes, etc
spellingShingle GE Environmental Sciences
RS Pharmacy and materia medica
SB Plant culture
TP248.13 Biotechnology
TP934 Paints, pigments, varnishes, etc
Othman, Rashidi
Ramya, Razanah
Mohd Hassan, Norazian
Kammona, Suhair
Qualitative and Quantitative Phenolic Compounds Analysis of Dicranopteris linearis Different Fractional Polarities Leaves Extract
description Dicranopteris linearis occupies in an open ground that contains poor soils and often colonizing disturbed space that partly shaded area. It has been known for various traditional values including medicinal, edible food, soil erosion protection, pen and furniture. Even though the plants possess both economic and medicinal value, they still form the neglected group of a plant. The present study was carried out to characterize the phenolic compounds in D. linearis leaves extract in different fractional polarities qualitative and quantitatively. Dried leaves of D. linearis were successfully extracted by using water extraction before separated by petroleum ether, ethyl acetate and butanol fractions. All the fractional extracts have been analysed by using GCTOF-MS and HPLC. The result from GCTOF-MS analysis of fractional extracts showed 38 compounds found in petroleum ether, ethyl acetate and butanol extracts. However, only four phenolic compounds were identified through HPLC analysis in ethyl acetate and butanol extracts which were 2-Methoxy-4-vinylphenol, Vanillin, 4-Hydroxybenzaldehyde and 4-Hydroxybenzohydrazide. The results revealed that D. linearis contained 699.83 ± 6.26 μg GAE /g DW of total phenolic acid whereas individual phenolic acids were predominantly caffeic acid (0.44 ± 0.01 μg/g DW) and ferulic acid (0.22 ± 0.00 μg/g DW) in ethyl acetate and caffeic acid (0.10 ± 0.00 μg/g DW) and 2-Coumaric acid (0.44 ± 0.00 μg/g DW) in butanol extracts. In the present study, the plant extracts demonstrated the highest phenolic compound detected in ethyl acetate and butanol compared to petroleum ether extract.
format Article
author Othman, Rashidi
Ramya, Razanah
Mohd Hassan, Norazian
Kammona, Suhair
author_facet Othman, Rashidi
Ramya, Razanah
Mohd Hassan, Norazian
Kammona, Suhair
author_sort Othman, Rashidi
title Qualitative and Quantitative Phenolic Compounds Analysis of Dicranopteris linearis Different Fractional Polarities Leaves Extract
title_short Qualitative and Quantitative Phenolic Compounds Analysis of Dicranopteris linearis Different Fractional Polarities Leaves Extract
title_full Qualitative and Quantitative Phenolic Compounds Analysis of Dicranopteris linearis Different Fractional Polarities Leaves Extract
title_fullStr Qualitative and Quantitative Phenolic Compounds Analysis of Dicranopteris linearis Different Fractional Polarities Leaves Extract
title_full_unstemmed Qualitative and Quantitative Phenolic Compounds Analysis of Dicranopteris linearis Different Fractional Polarities Leaves Extract
title_sort qualitative and quantitative phenolic compounds analysis of dicranopteris linearis different fractional polarities leaves extract
publisher SET PUBLISHER
publishDate 2020
url http://irep.iium.edu.my/78491/
http://irep.iium.edu.my/78491/1/JPANSV10N1A2-Othman.pdf
first_indexed 2023-09-18T21:50:35Z
last_indexed 2023-09-18T21:50:35Z
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