GCTOF-MS and HPLC Identification of Phenolic Compounds with Different Fractional Extracts of Lepironia articulata

Cyperaceae species have an intrinsic value as a source of active elements with biological activity from the family of monocotyledonous known as sedges. Sedges grow in all types of soils associated with wetlands or poor soils. The aim of this present study is to evaluate the content of phenolic comp...

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Main Authors:	Othman, Rashidi, Ramya, Razanah, Mohd Hassan, Norazian, Kammona, Suhair
Format:	Article
Language:	English
Published:	SET PUBLISHER 2020
Subjects:	GE Environmental Sciences RS Pharmacy and materia medica SB Plant culture TP248.13 Biotechnology TP934 Paints, pigments, varnishes, etc
Online Access:	http://irep.iium.edu.my/78490/ http://irep.iium.edu.my/78490/1/JPANSV10N1A1-Othman.pdf

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spelling	iium-784902020-02-19T03:04:27Z http://irep.iium.edu.my/78490/ GCTOF-MS and HPLC Identification of Phenolic Compounds with Different Fractional Extracts of Lepironia articulata Othman, Rashidi Ramya, Razanah Mohd Hassan, Norazian Kammona, Suhair GE Environmental Sciences RS Pharmacy and materia medica SB Plant culture TP248.13 Biotechnology TP934 Paints, pigments, varnishes, etc Cyperaceae species have an intrinsic value as a source of active elements with biological activity from the family of monocotyledonous known as sedges. Sedges grow in all types of soils associated with wetlands or poor soils. The aim of this present study is to evaluate the content of phenolic compounds by qualitative and quantitive analysis on Lepironia articulata. Dried leaves of L. articulata were successfully extracted by using water extraction then separated with different solvent polarities; petroleum ether, ethyl acetate and butanol fractions before being analysed using GCTOF-MS, microplate reader and HPLC. The result from the GCTOF-MS analysis of fractional extracts showed that 48 compounds were found in petroleum ether, ethyl acetate and butanol extracts. From those extracts, only six phenolic compounds were identified in ethyl acetate and butanol extracts which were 2-Methoxy-4-vinylphenol, Phenol, 2,4- bis(1,1-dimethylethyl)-, 4-Hydroxybenzaldehyde, Catechol, Phenol, 2-methoxy- and Vanillin. The total phenolic content was found to be 984.63 ± 5.96 μg GAE/g DW in L. articulata. Quantitative analysis of individual phenolic acid by HPLC showed the predominant amount of Vanillic acid (0.48 ± 0.00 μg/g DW) in ethyl acetate while 4-Hydroxybenzoic acid and Caffeic acid, both of which were 0.12 ± 0.00 μg/g DW in butanol extracts. In the present study, the plant extracts demonstrated the highest phenolic compound detected in ethyl acetate and butanol. SET PUBLISHER 2020-02-04 Article PeerReviewed application/pdf en http://irep.iium.edu.my/78490/1/JPANSV10N1A1-Othman.pdf Othman, Rashidi and Ramya, Razanah and Mohd Hassan, Norazian and Kammona, Suhair (2020) GCTOF-MS and HPLC Identification of Phenolic Compounds with Different Fractional Extracts of Lepironia articulata. Journal of Pharmacy and Nutrition Sciences, 10 (1). pp. 1-6. ISSN 2223-3806 E-ISSN 927-5951
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	GE Environmental Sciences RS Pharmacy and materia medica SB Plant culture TP248.13 Biotechnology TP934 Paints, pigments, varnishes, etc
spellingShingle	GE Environmental Sciences RS Pharmacy and materia medica SB Plant culture TP248.13 Biotechnology TP934 Paints, pigments, varnishes, etc Othman, Rashidi Ramya, Razanah Mohd Hassan, Norazian Kammona, Suhair GCTOF-MS and HPLC Identification of Phenolic Compounds with Different Fractional Extracts of Lepironia articulata
description	Cyperaceae species have an intrinsic value as a source of active elements with biological activity from the family of monocotyledonous known as sedges. Sedges grow in all types of soils associated with wetlands or poor soils. The aim of this present study is to evaluate the content of phenolic compounds by qualitative and quantitive analysis on Lepironia articulata. Dried leaves of L. articulata were successfully extracted by using water extraction then separated with different solvent polarities; petroleum ether, ethyl acetate and butanol fractions before being analysed using GCTOF-MS, microplate reader and HPLC. The result from the GCTOF-MS analysis of fractional extracts showed that 48 compounds were found in petroleum ether, ethyl acetate and butanol extracts. From those extracts, only six phenolic compounds were identified in ethyl acetate and butanol extracts which were 2-Methoxy-4-vinylphenol, Phenol, 2,4- bis(1,1-dimethylethyl)-, 4-Hydroxybenzaldehyde, Catechol, Phenol, 2-methoxy- and Vanillin. The total phenolic content was found to be 984.63 ± 5.96 μg GAE/g DW in L. articulata. Quantitative analysis of individual phenolic acid by HPLC showed the predominant amount of Vanillic acid (0.48 ± 0.00 μg/g DW) in ethyl acetate while 4-Hydroxybenzoic acid and Caffeic acid, both of which were 0.12 ± 0.00 μg/g DW in butanol extracts. In the present study, the plant extracts demonstrated the highest phenolic compound detected in ethyl acetate and butanol.
format	Article
author	Othman, Rashidi Ramya, Razanah Mohd Hassan, Norazian Kammona, Suhair
author_facet	Othman, Rashidi Ramya, Razanah Mohd Hassan, Norazian Kammona, Suhair
author_sort	Othman, Rashidi
title	GCTOF-MS and HPLC Identification of Phenolic Compounds with Different Fractional Extracts of Lepironia articulata
title_short	GCTOF-MS and HPLC Identification of Phenolic Compounds with Different Fractional Extracts of Lepironia articulata
title_full	GCTOF-MS and HPLC Identification of Phenolic Compounds with Different Fractional Extracts of Lepironia articulata
title_fullStr	GCTOF-MS and HPLC Identification of Phenolic Compounds with Different Fractional Extracts of Lepironia articulata
title_full_unstemmed	GCTOF-MS and HPLC Identification of Phenolic Compounds with Different Fractional Extracts of Lepironia articulata
title_sort	gctof-ms and hplc identification of phenolic compounds with different fractional extracts of lepironia articulata
publisher	SET PUBLISHER
publishDate	2020
url	http://irep.iium.edu.my/78490/ http://irep.iium.edu.my/78490/1/JPANSV10N1A1-Othman.pdf
first_indexed	2023-09-18T21:50:35Z
last_indexed	2023-09-18T21:50:35Z
_version_	1777413742025244672

GCTOF-MS and HPLC Identification of Phenolic Compounds with Different Fractional Extracts of Lepironia articulata

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