The effect of low dose organic arsenic exposure on inflammatory genes expression in rat’s kidney

Monosodium methylarsonate (MSMA) is an organic arsenical pesticide widely used in agriculture. Exposure to arsenic has been linked with multiple health problems. Inflammatory genes such as interleukin 6 (IL-6) and interleukin 8 (IL-8) play an important role in the pathophysiology of exposure to an a...

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Main Authors:	Wan Salleh, Wan Muhamad Salahudin, A.Talib, Norlelawati, Abdullah, Nor Zamzila, Buyong, Zunariah
Format:	Article
Language:	English
Published:	Kulliyyah of Allied Health Sciences, International Islamic University Malaysia 2019
Subjects:	RB Pathology
Online Access:	http://irep.iium.edu.my/78364/ http://irep.iium.edu.my/78364/ http://irep.iium.edu.my/78364/1/78364_The%20effect%20of%20low%20dose%20organic%20arsenic.pdf

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spelling	iium-783642020-01-30T01:40:38Z http://irep.iium.edu.my/78364/ The effect of low dose organic arsenic exposure on inflammatory genes expression in rat’s kidney Wan Salleh, Wan Muhamad Salahudin A.Talib, Norlelawati Abdullah, Nor Zamzila Buyong, Zunariah RB Pathology Monosodium methylarsonate (MSMA) is an organic arsenical pesticide widely used in agriculture. Exposure to arsenic has been linked with multiple health problems. Inflammatory genes such as interleukin 6 (IL-6) and interleukin 8 (IL-8) play an important role in the pathophysiology of exposure to an acute high dose arsenic-mediated nephrotoxicity, which led to the proximal tubular injury. However, studies focusing on low dose organic arsenic exposure and its adverse effects on kidneys are limited. This study aimed to evaluate the effects of low dose arsenic exposure on the inflammatory genes expression in rats’ kidneys at three different duration intervals; 2 months, four months and six months. Thirty-six male Sprague-Dawley rats were randomly divided into six groups (n=6); a treatment group and its control for each interval. The treatment groups were given daily oral gavage of MSMA at 63.0 mg/kg body weight (BW) which is equivalent to 1/20 LD50 of MSMA. While control groups received distilled water via oral gavage. At the end of study intervals, the kidney tissues were harvested for arsenic level analysis and molecular analysis. The RNA integrity was confirmed with Qiaxcel analysis. The expressions of inflammatory genes were analysed using RT2 SYBR Green qPCR Mastermix. Tissue arsenic concentration was higher in all treated group. Both IL-6 and IL-8 showed a similar pattern of expressions. Organic arsenic down-regulated IL-6 and IL-8 in 2-month (both fold change -1.03) and 6-month groups (fold change -1.36,-1.15). However, in the 4-month group, both IL-6 and IL-8 were up-regulated (both fold change 1.31). Interestingly, these findings suggest that low dose arsenic exposure has shown the anti-inflammatory effect at 2-month and 6-month. However, 4-month paradoxically demonstrated a pro-inflammatory effect consistent with the tissue arsenic levels. Kulliyyah of Allied Health Sciences, International Islamic University Malaysia 2019-12-29 Article PeerReviewed application/pdf en http://irep.iium.edu.my/78364/1/78364_The%20effect%20of%20low%20dose%20organic%20arsenic.pdf Wan Salleh, Wan Muhamad Salahudin and A.Talib, Norlelawati and Abdullah, Nor Zamzila and Buyong, Zunariah (2019) The effect of low dose organic arsenic exposure on inflammatory genes expression in rat’s kidney. International Journal of Allied Health Sciences, 3 (3). p. 845. E-ISSN 2600-8491 https://journals.iium.edu.my/ijahs/index.php/IJAHS/article/view/408
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RB Pathology
spellingShingle	RB Pathology Wan Salleh, Wan Muhamad Salahudin A.Talib, Norlelawati Abdullah, Nor Zamzila Buyong, Zunariah The effect of low dose organic arsenic exposure on inflammatory genes expression in rat’s kidney
description	Monosodium methylarsonate (MSMA) is an organic arsenical pesticide widely used in agriculture. Exposure to arsenic has been linked with multiple health problems. Inflammatory genes such as interleukin 6 (IL-6) and interleukin 8 (IL-8) play an important role in the pathophysiology of exposure to an acute high dose arsenic-mediated nephrotoxicity, which led to the proximal tubular injury. However, studies focusing on low dose organic arsenic exposure and its adverse effects on kidneys are limited. This study aimed to evaluate the effects of low dose arsenic exposure on the inflammatory genes expression in rats’ kidneys at three different duration intervals; 2 months, four months and six months. Thirty-six male Sprague-Dawley rats were randomly divided into six groups (n=6); a treatment group and its control for each interval. The treatment groups were given daily oral gavage of MSMA at 63.0 mg/kg body weight (BW) which is equivalent to 1/20 LD50 of MSMA. While control groups received distilled water via oral gavage. At the end of study intervals, the kidney tissues were harvested for arsenic level analysis and molecular analysis. The RNA integrity was confirmed with Qiaxcel analysis. The expressions of inflammatory genes were analysed using RT2 SYBR Green qPCR Mastermix. Tissue arsenic concentration was higher in all treated group. Both IL-6 and IL-8 showed a similar pattern of expressions. Organic arsenic down-regulated IL-6 and IL-8 in 2-month (both fold change -1.03) and 6-month groups (fold change -1.36,-1.15). However, in the 4-month group, both IL-6 and IL-8 were up-regulated (both fold change 1.31). Interestingly, these findings suggest that low dose arsenic exposure has shown the anti-inflammatory effect at 2-month and 6-month. However, 4-month paradoxically demonstrated a pro-inflammatory effect consistent with the tissue arsenic levels.
format	Article
author	Wan Salleh, Wan Muhamad Salahudin A.Talib, Norlelawati Abdullah, Nor Zamzila Buyong, Zunariah
author_facet	Wan Salleh, Wan Muhamad Salahudin A.Talib, Norlelawati Abdullah, Nor Zamzila Buyong, Zunariah
author_sort	Wan Salleh, Wan Muhamad Salahudin
title	The effect of low dose organic arsenic exposure on inflammatory genes expression in rat’s kidney
title_short	The effect of low dose organic arsenic exposure on inflammatory genes expression in rat’s kidney
title_full	The effect of low dose organic arsenic exposure on inflammatory genes expression in rat’s kidney
title_fullStr	The effect of low dose organic arsenic exposure on inflammatory genes expression in rat’s kidney
title_full_unstemmed	The effect of low dose organic arsenic exposure on inflammatory genes expression in rat’s kidney
title_sort	effect of low dose organic arsenic exposure on inflammatory genes expression in rat’s kidney
publisher	Kulliyyah of Allied Health Sciences, International Islamic University Malaysia
publishDate	2019
url	http://irep.iium.edu.my/78364/ http://irep.iium.edu.my/78364/ http://irep.iium.edu.my/78364/1/78364_The%20effect%20of%20low%20dose%20organic%20arsenic.pdf
first_indexed	2023-09-18T21:50:26Z
last_indexed	2023-09-18T21:50:26Z
_version_	1777413732869079040

The effect of low dose organic arsenic exposure on inflammatory genes expression in rat’s kidney

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