Systematic significance of leaf epidermal characterictics in Shorea Roxb. (Dipterocarpaceae) in Malaysia

The taxonomic problems in Shorea mostly involve identification and classification. The investigation presented in this study aims to prove that leaf epidermal characteristics have taxonomic value in Shorea. Epidermal peels were prepared using Jeffrey’s solution. The underside of the leaf surface...

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Main Authors:	Che Amri, Che Nurul Aini, Talip, Noraini
Format:	Conference or Workshop Item
Language:	English English
Published:	2019
Subjects:	QK Botany
Online Access:	http://irep.iium.edu.my/78314/ http://irep.iium.edu.my/78314/7/Abstract%20Oral%20LS.pdf http://irep.iium.edu.my/78314/9/SCMSM%202019%20%28UPDATE%29.pdf

id	iium-78314
recordtype	eprints
spelling	iium-783142020-02-15T10:31:42Z http://irep.iium.edu.my/78314/ Systematic significance of leaf epidermal characterictics in Shorea Roxb. (Dipterocarpaceae) in Malaysia Che Amri, Che Nurul Aini Talip, Noraini QK Botany The taxonomic problems in Shorea mostly involve identification and classification. The investigation presented in this study aims to prove that leaf epidermal characteristics have taxonomic value in Shorea. Epidermal peels were prepared using Jeffrey’s solution. The underside of the leaf surface was scraped with a razor blade to remove lose cells and soaked for several days in Jeffrey’s solution or bleaching agent Vortex. The cleared leaf was washed, stained with Safranin, dehydrated, mounted, photographed and observed as described. For observation under scanning electron microscope, 3 to 5 mm2 leaf sections were washed in water, dehydrated through an alcohol series, were then placed between two glass slides, undergone critical point drying process, coated with gold palladium and were examined in a 2.7Å field emission of Cambridge Instruments Stereoscan 360 scanning electron microscope. Results have shown that the epidermis of the leaf surface under LM is useful for distinguishing S. isoptera, and S. maxwelliana, from the others and also between these two species themselves, so that it could be a diagnostic character. The study of the epidermal surfaces revealed a number of important micromorphological characters, and these characters exhibit interesting interspecific variations that are of significance for species identification and authentification. The presence of crustose wax with an abundance of flakes on the leaf surface may be characteristic of S. parviflora. The epidermal sculpturing has considerable diagnostic value, and may characterize some species and could serve as a criterion of distinguishing S. bracteolata. Results have shown that leaf epidermal features could be useful for authentification and identification purposes especially at species level, thus this study have shown taxonomic value of leaf epidermal characteristics in Shorea. 2019-12-18 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/78314/7/Abstract%20Oral%20LS.pdf application/pdf en http://irep.iium.edu.my/78314/9/SCMSM%202019%20%28UPDATE%29.pdf Che Amri, Che Nurul Aini and Talip, Noraini (2019) Systematic significance of leaf epidermal characterictics in Shorea Roxb. (Dipterocarpaceae) in Malaysia. In: 28th Scientific Conference of Microscopy Society Malaysia (28th SCMSM 2019), 18-20 Dec 2019, Swiss Garden Beach Resort, Pahang, Malaysia. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	QK Botany
spellingShingle	QK Botany Che Amri, Che Nurul Aini Talip, Noraini Systematic significance of leaf epidermal characterictics in Shorea Roxb. (Dipterocarpaceae) in Malaysia
description	The taxonomic problems in Shorea mostly involve identification and classification. The investigation presented in this study aims to prove that leaf epidermal characteristics have taxonomic value in Shorea. Epidermal peels were prepared using Jeffrey’s solution. The underside of the leaf surface was scraped with a razor blade to remove lose cells and soaked for several days in Jeffrey’s solution or bleaching agent Vortex. The cleared leaf was washed, stained with Safranin, dehydrated, mounted, photographed and observed as described. For observation under scanning electron microscope, 3 to 5 mm2 leaf sections were washed in water, dehydrated through an alcohol series, were then placed between two glass slides, undergone critical point drying process, coated with gold palladium and were examined in a 2.7Å field emission of Cambridge Instruments Stereoscan 360 scanning electron microscope. Results have shown that the epidermis of the leaf surface under LM is useful for distinguishing S. isoptera, and S. maxwelliana, from the others and also between these two species themselves, so that it could be a diagnostic character. The study of the epidermal surfaces revealed a number of important micromorphological characters, and these characters exhibit interesting interspecific variations that are of significance for species identification and authentification. The presence of crustose wax with an abundance of flakes on the leaf surface may be characteristic of S. parviflora. The epidermal sculpturing has considerable diagnostic value, and may characterize some species and could serve as a criterion of distinguishing S. bracteolata. Results have shown that leaf epidermal features could be useful for authentification and identification purposes especially at species level, thus this study have shown taxonomic value of leaf epidermal characteristics in Shorea.
format	Conference or Workshop Item
author	Che Amri, Che Nurul Aini Talip, Noraini
author_facet	Che Amri, Che Nurul Aini Talip, Noraini
author_sort	Che Amri, Che Nurul Aini
title	Systematic significance of leaf epidermal characterictics in Shorea Roxb. (Dipterocarpaceae) in Malaysia
title_short	Systematic significance of leaf epidermal characterictics in Shorea Roxb. (Dipterocarpaceae) in Malaysia
title_full	Systematic significance of leaf epidermal characterictics in Shorea Roxb. (Dipterocarpaceae) in Malaysia
title_fullStr	Systematic significance of leaf epidermal characterictics in Shorea Roxb. (Dipterocarpaceae) in Malaysia
title_full_unstemmed	Systematic significance of leaf epidermal characterictics in Shorea Roxb. (Dipterocarpaceae) in Malaysia
title_sort	systematic significance of leaf epidermal characterictics in shorea roxb. (dipterocarpaceae) in malaysia
publishDate	2019
url	http://irep.iium.edu.my/78314/ http://irep.iium.edu.my/78314/7/Abstract%20Oral%20LS.pdf http://irep.iium.edu.my/78314/9/SCMSM%202019%20%28UPDATE%29.pdf
first_indexed	2023-09-18T21:50:21Z
last_indexed	2023-09-18T21:50:21Z
_version_	1777413727262343168

Systematic significance of leaf epidermal characterictics in Shorea Roxb. (Dipterocarpaceae) in Malaysia

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