Efficacy of bath vaccination with a live attenuated Vibrio harveyi against vibriosis in Asian seabass fingerling, Lates calcarifer

Vibrio harveyi causes vibriosis in various marine aquaculture fish species, especially when they are young. The infection subsequently leads to significant economic losses for aquaculture farms. Vaccination is recommended to control this disease. This study describes the efficacy of a live attenuate...

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Main Authors:	Yong, Kit Chin, Al-saari, Nurhidayu, Zulperi, Zarirah, Mohd-Aris, Aslizah, Salleh, Annas, Silvara, Santha, Mohamad, Aslah, JingYie, Lee, Saad, Mohd Zamri, Ina Salwany, Md Yasin
Format:	Article
Language:	English English English
Published:	Blackwell Publishing Ltd 2020
Subjects:	S Agriculture (General) SH Aquaculture. Fisheries. Angling
Online Access:	http://irep.iium.edu.my/78218/ http://irep.iium.edu.my/78218/ http://irep.iium.edu.my/78218/ http://irep.iium.edu.my/78218/1/78218_Efficacy%20of%20bath%20vaccination%20with%20a%20live%20attenuated.pdf http://irep.iium.edu.my/78218/7/78218_Efficacy%20of%20bath%20vaccination%20with%20a%20live_SCOPUS.pdf http://irep.iium.edu.my/78218/8/78218_Efficacy%20of%20bath%20vaccination%20with%20a%20live_WOS%20in%20press.pdf

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recordtype	eprints
spelling	iium-782182020-03-06T09:54:42Z http://irep.iium.edu.my/78218/ Efficacy of bath vaccination with a live attenuated Vibrio harveyi against vibriosis in Asian seabass fingerling, Lates calcarifer Yong, Kit Chin Al-saari, Nurhidayu Zulperi, Zarirah Mohd-Aris, Aslizah Salleh, Annas Silvara, Santha Mohamad, Aslah JingYie, Lee Saad, Mohd Zamri Ina Salwany, Md Yasin S Agriculture (General) SH Aquaculture. Fisheries. Angling Vibrio harveyi causes vibriosis in various marine aquaculture fish species, especially when they are young. The infection subsequently leads to significant economic losses for aquaculture farms. Vaccination is recommended to control this disease. This study describes the efficacy of a live attenuated V. harveyi strain MVh_vhs (LAVh) as a vaccine candidate in controlling infection by wild‐type V. harveyi (WTVh) in Lates calcarifer. A total of 240 fingerlings were divided into four groups. Group 1 was not vaccinated and was not challenged, Group 2 was vaccinated with a formalin‐killed V. harveyi (FKVh), Group 3 was vaccinated with the LAVh before challenge and Group 4 was not vaccinated and was challenged. Bath vaccination was employed for one hour before the LAVh distribution was determined in the fish mucus, gill, liver, gut, kidney and spleen. The gills, livers, kidneys and skins were also sampled for gene expression analysis. To challenge the fish, skin abrasion was conducted before the fish were challenged by immersion with WTVh. The results revealed an extensive distribution of the LAVh in the liver and kidneys of the fish in Group 3 for the first 12 hr, resulting in mild lesions compared with Group 1. Similarly, there were significantly (p < .05) higher expressions of the Chemokine ligand 4 and major histocompatibility complex I genes in the skin and liver of the fish in Group 3 in comparison with other groups. Vaccination with LAVh resulted in a significantly high rate of survival (68%) of the fingerlings after being challenged with WTVh. Blackwell Publishing Ltd 2020-01-01 Article PeerReviewed application/pdf en http://irep.iium.edu.my/78218/1/78218_Efficacy%20of%20bath%20vaccination%20with%20a%20live%20attenuated.pdf application/pdf en http://irep.iium.edu.my/78218/7/78218_Efficacy%20of%20bath%20vaccination%20with%20a%20live_SCOPUS.pdf application/pdf en http://irep.iium.edu.my/78218/8/78218_Efficacy%20of%20bath%20vaccination%20with%20a%20live_WOS%20in%20press.pdf Yong, Kit Chin and Al-saari, Nurhidayu and Zulperi, Zarirah and Mohd-Aris, Aslizah and Salleh, Annas and Silvara, Santha and Mohamad, Aslah and JingYie, Lee and Saad, Mohd Zamri and Ina Salwany, Md Yasin (2020) Efficacy of bath vaccination with a live attenuated Vibrio harveyi against vibriosis in Asian seabass fingerling, Lates calcarifer. Aquaculture Research, 51 (1). pp. 389-399. ISSN 1355-557X https://onlinelibrary.wiley.com/doi/pdf/10.1111/are.14386 https://doi.org/10.1111/are.14386
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English
topic	S Agriculture (General) SH Aquaculture. Fisheries. Angling
spellingShingle	S Agriculture (General) SH Aquaculture. Fisheries. Angling Yong, Kit Chin Al-saari, Nurhidayu Zulperi, Zarirah Mohd-Aris, Aslizah Salleh, Annas Silvara, Santha Mohamad, Aslah JingYie, Lee Saad, Mohd Zamri Ina Salwany, Md Yasin Efficacy of bath vaccination with a live attenuated Vibrio harveyi against vibriosis in Asian seabass fingerling, Lates calcarifer
description	Vibrio harveyi causes vibriosis in various marine aquaculture fish species, especially when they are young. The infection subsequently leads to significant economic losses for aquaculture farms. Vaccination is recommended to control this disease. This study describes the efficacy of a live attenuated V. harveyi strain MVh_vhs (LAVh) as a vaccine candidate in controlling infection by wild‐type V. harveyi (WTVh) in Lates calcarifer. A total of 240 fingerlings were divided into four groups. Group 1 was not vaccinated and was not challenged, Group 2 was vaccinated with a formalin‐killed V. harveyi (FKVh), Group 3 was vaccinated with the LAVh before challenge and Group 4 was not vaccinated and was challenged. Bath vaccination was employed for one hour before the LAVh distribution was determined in the fish mucus, gill, liver, gut, kidney and spleen. The gills, livers, kidneys and skins were also sampled for gene expression analysis. To challenge the fish, skin abrasion was conducted before the fish were challenged by immersion with WTVh. The results revealed an extensive distribution of the LAVh in the liver and kidneys of the fish in Group 3 for the first 12 hr, resulting in mild lesions compared with Group 1. Similarly, there were significantly (p < .05) higher expressions of the Chemokine ligand 4 and major histocompatibility complex I genes in the skin and liver of the fish in Group 3 in comparison with other groups. Vaccination with LAVh resulted in a significantly high rate of survival (68%) of the fingerlings after being challenged with WTVh.
format	Article
author	Yong, Kit Chin Al-saari, Nurhidayu Zulperi, Zarirah Mohd-Aris, Aslizah Salleh, Annas Silvara, Santha Mohamad, Aslah JingYie, Lee Saad, Mohd Zamri Ina Salwany, Md Yasin
author_facet	Yong, Kit Chin Al-saari, Nurhidayu Zulperi, Zarirah Mohd-Aris, Aslizah Salleh, Annas Silvara, Santha Mohamad, Aslah JingYie, Lee Saad, Mohd Zamri Ina Salwany, Md Yasin
author_sort	Yong, Kit Chin
title	Efficacy of bath vaccination with a live attenuated Vibrio harveyi against vibriosis in Asian seabass fingerling, Lates calcarifer
title_short	Efficacy of bath vaccination with a live attenuated Vibrio harveyi against vibriosis in Asian seabass fingerling, Lates calcarifer
title_full	Efficacy of bath vaccination with a live attenuated Vibrio harveyi against vibriosis in Asian seabass fingerling, Lates calcarifer
title_fullStr	Efficacy of bath vaccination with a live attenuated Vibrio harveyi against vibriosis in Asian seabass fingerling, Lates calcarifer
title_full_unstemmed	Efficacy of bath vaccination with a live attenuated Vibrio harveyi against vibriosis in Asian seabass fingerling, Lates calcarifer
title_sort	efficacy of bath vaccination with a live attenuated vibrio harveyi against vibriosis in asian seabass fingerling, lates calcarifer
publisher	Blackwell Publishing Ltd
publishDate	2020
url	http://irep.iium.edu.my/78218/ http://irep.iium.edu.my/78218/ http://irep.iium.edu.my/78218/ http://irep.iium.edu.my/78218/1/78218_Efficacy%20of%20bath%20vaccination%20with%20a%20live%20attenuated.pdf http://irep.iium.edu.my/78218/7/78218_Efficacy%20of%20bath%20vaccination%20with%20a%20live_SCOPUS.pdf http://irep.iium.edu.my/78218/8/78218_Efficacy%20of%20bath%20vaccination%20with%20a%20live_WOS%20in%20press.pdf
first_indexed	2023-09-18T21:50:15Z
last_indexed	2023-09-18T21:50:15Z
_version_	1777413720827232256

Efficacy of bath vaccination with a live attenuated Vibrio harveyi against vibriosis in Asian seabass fingerling, Lates calcarifer

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