The effect of oral probiotic Streptococcus salivarius K12 on Candida albicans biofilm formation

Introduction: Oral cancer is the sixth most common cancer worldwide with Candida albicans infection has been one of aetiological factors for the disease. Meanwhile, Streptococcus salivarius K12 is an oral probiotic that is beneficial to the oral cavity. The objective of the present study is to deter...

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Main Authors:	Mokhtar, Munirah, Rismayuddin, Alia Risma, Abdul Wahab, Ridhwan, Rostam, Muhamad Ashraf, Mohd Nasir, Mohd Hamzah, Arzmi, Mohd Hafiz
Format:	Conference or Workshop Item
Language:	English English
Published:	2019
Subjects:	QR Microbiology
Online Access:	http://irep.iium.edu.my/78175/ http://irep.iium.edu.my/78175/1/MRS%20abstract.jpeg http://irep.iium.edu.my/78175/2/MRS%202019%20Abstract%20%26%20Programme%20Book%20v1-2.pdf

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spelling	iium-781752020-01-28T05:12:57Z http://irep.iium.edu.my/78175/ The effect of oral probiotic Streptococcus salivarius K12 on Candida albicans biofilm formation Mokhtar, Munirah Rismayuddin, Alia Risma Abdul Wahab, Ridhwan Rostam, Muhamad Ashraf Mohd Nasir, Mohd Hamzah Arzmi, Mohd Hafiz QR Microbiology Introduction: Oral cancer is the sixth most common cancer worldwide with Candida albicans infection has been one of aetiological factors for the disease. Meanwhile, Streptococcus salivarius K12 is an oral probiotic that is beneficial to the oral cavity. The objective of the present study is to determine the effect of S. salivarius K12 on C. albicans biofilm forming ability with the hypothesis that S. salivarius K12 inhibits biofilm formation of C. albicans. Materials and Method: To assess the effect of S. salivarius K12 on C. albicans biofilm formation, S. salivarius K12, lab strain C. albicans MYA-4901 and cancer isolates; ALC2 and ALC3 were grown in nutrient broth (NB) and RPMI. To develop mono-species biofilm, C. albicans and S. salivarius K12 were standardised to 105 cells and 106 cells, respectively and grown in 96-well plate. Polymicrobial biofilms were developed by inoculating both microorganisms in a same well with similar cell number as in mono-species. The biofilms were incubated for 72 hours at 37 °C and the media were replenished every 24 hours. Finally, crystal violet assay was conducted, and the optical density was measured at OD620nm. Results: Polymicrobial biofilms of C. albicans (MYA-4901 and ALC3) with S. salivarius K12 when grown in NB, exhibited decrease by 64.5±40.3% and 83.7±5.4%, respectively when compared to the expected biofilms which were predominanted by yeast form. Furthermore, polymicrobial biofilms of C. albicans (ALC2 and ALC3) with S. salivarius K12 showed decrease by 62.5±25.6% and 55.9±17.1% ,respectively when compared to the expected biofilms when grown in RPMI that were predominanted by hyphal form. Conclusion: S. salivarius K12 inhibited polymicrobial biofilms formation of C. albicans in both yeast and hyphal forms, thus supported the hypothesis that S. salivarius K12 inhibits biofilm formation of C. albicans. 2019 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/78175/1/MRS%20abstract.jpeg application/pdf en http://irep.iium.edu.my/78175/2/MRS%202019%20Abstract%20%26%20Programme%20Book%20v1-2.pdf Mokhtar, Munirah and Rismayuddin, Alia Risma and Abdul Wahab, Ridhwan and Rostam, Muhamad Ashraf and Mohd Nasir, Mohd Hamzah and Arzmi, Mohd Hafiz (2019) The effect of oral probiotic Streptococcus salivarius K12 on Candida albicans biofilm formation. In: Medical Research Symposium 2019: Expanding Research Collaboration in Health, 9 October 2019, IIUM Medical Centre, Kuantan Pahang.
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	QR Microbiology
spellingShingle	QR Microbiology Mokhtar, Munirah Rismayuddin, Alia Risma Abdul Wahab, Ridhwan Rostam, Muhamad Ashraf Mohd Nasir, Mohd Hamzah Arzmi, Mohd Hafiz The effect of oral probiotic Streptococcus salivarius K12 on Candida albicans biofilm formation
description	Introduction: Oral cancer is the sixth most common cancer worldwide with Candida albicans infection has been one of aetiological factors for the disease. Meanwhile, Streptococcus salivarius K12 is an oral probiotic that is beneficial to the oral cavity. The objective of the present study is to determine the effect of S. salivarius K12 on C. albicans biofilm forming ability with the hypothesis that S. salivarius K12 inhibits biofilm formation of C. albicans. Materials and Method: To assess the effect of S. salivarius K12 on C. albicans biofilm formation, S. salivarius K12, lab strain C. albicans MYA-4901 and cancer isolates; ALC2 and ALC3 were grown in nutrient broth (NB) and RPMI. To develop mono-species biofilm, C. albicans and S. salivarius K12 were standardised to 105 cells and 106 cells, respectively and grown in 96-well plate. Polymicrobial biofilms were developed by inoculating both microorganisms in a same well with similar cell number as in mono-species. The biofilms were incubated for 72 hours at 37 °C and the media were replenished every 24 hours. Finally, crystal violet assay was conducted, and the optical density was measured at OD620nm. Results: Polymicrobial biofilms of C. albicans (MYA-4901 and ALC3) with S. salivarius K12 when grown in NB, exhibited decrease by 64.5±40.3% and 83.7±5.4%, respectively when compared to the expected biofilms which were predominanted by yeast form. Furthermore, polymicrobial biofilms of C. albicans (ALC2 and ALC3) with S. salivarius K12 showed decrease by 62.5±25.6% and 55.9±17.1% ,respectively when compared to the expected biofilms when grown in RPMI that were predominanted by hyphal form. Conclusion: S. salivarius K12 inhibited polymicrobial biofilms formation of C. albicans in both yeast and hyphal forms, thus supported the hypothesis that S. salivarius K12 inhibits biofilm formation of C. albicans.
format	Conference or Workshop Item
author	Mokhtar, Munirah Rismayuddin, Alia Risma Abdul Wahab, Ridhwan Rostam, Muhamad Ashraf Mohd Nasir, Mohd Hamzah Arzmi, Mohd Hafiz
author_facet	Mokhtar, Munirah Rismayuddin, Alia Risma Abdul Wahab, Ridhwan Rostam, Muhamad Ashraf Mohd Nasir, Mohd Hamzah Arzmi, Mohd Hafiz
author_sort	Mokhtar, Munirah
title	The effect of oral probiotic Streptococcus salivarius K12 on Candida albicans biofilm formation
title_short	The effect of oral probiotic Streptococcus salivarius K12 on Candida albicans biofilm formation
title_full	The effect of oral probiotic Streptococcus salivarius K12 on Candida albicans biofilm formation
title_fullStr	The effect of oral probiotic Streptococcus salivarius K12 on Candida albicans biofilm formation
title_full_unstemmed	The effect of oral probiotic Streptococcus salivarius K12 on Candida albicans biofilm formation
title_sort	effect of oral probiotic streptococcus salivarius k12 on candida albicans biofilm formation
publishDate	2019
url	http://irep.iium.edu.my/78175/ http://irep.iium.edu.my/78175/1/MRS%20abstract.jpeg http://irep.iium.edu.my/78175/2/MRS%202019%20Abstract%20%26%20Programme%20Book%20v1-2.pdf
first_indexed	2023-09-18T21:50:11Z
last_indexed	2023-09-18T21:50:11Z
_version_	1777413716485079040

The effect of oral probiotic Streptococcus salivarius K12 on Candida albicans biofilm formation

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