Seronegative occult Hepatitis C virus infection (OCI) in a main haemodialysis centre In Pahang, Malaysia

Introduction: Occult HCV infection has a predilection for specific populations such as haemodialysis (HD) patients. The exact natural course, epidemiology, pathogenesis and clinical importance of OCI are unknown. We investigated the existence of OCI among local patients undergoing routine HD at a re...

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Main Authors:	Abdul Rahman, Siti Nurul Fazlin, Hamzah, Hairul Aini, Mustafa Mahmoud, Mohammed Imad Al-Deen
Format:	Article
Language:	English
Published:	IIUM Press 2019
Subjects:	QR Microbiology QR355 Virology
Online Access:	http://irep.iium.edu.my/78061/ http://irep.iium.edu.my/78061/ http://irep.iium.edu.my/78061/1/Fazlin%20IMJM%202019.pdf

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recordtype	eprints
spelling	iium-780612020-01-29T02:45:32Z http://irep.iium.edu.my/78061/ Seronegative occult Hepatitis C virus infection (OCI) in a main haemodialysis centre In Pahang, Malaysia Abdul Rahman, Siti Nurul Fazlin Hamzah, Hairul Aini Mustafa Mahmoud, Mohammed Imad Al-Deen QR Microbiology QR355 Virology Introduction: Occult HCV infection has a predilection for specific populations such as haemodialysis (HD) patients. The exact natural course, epidemiology, pathogenesis and clinical importance of OCI are unknown. We investigated the existence of OCI among local patients undergoing routine HD at a referral hospital in Pahang, Malaysia. Methods: Serum and peripheral blood mononuclear cells (PMBCs) were collected from peripheral venous blood samples of seropositive (anti-HCV positive) and seronegative (anti-HCV negative) HD patients as well as healthy individuals (negative control group). Inclusion criteria for the seronegative patients included elevated liver enzymes. Both conventional PCR and strand-specific PCR were used to detect the viral RNA and to indicate active viral replication in PBMCs respectively. Direct DNA sequencing was done to confirm the viral HCV RNA and their genotypes. Results: In the majority (90-100%) of seropositive chronic hepatitis C patients, viral RNA was detected in both serum and PMBCs . Meanwhile, out of 22 seronegative patients, 6 (27%) showed active viral replication in PBMCs but no detectable viral RNA presence in the serum. None of the negative control group had detectable viral RNA. All seronegative patients with OCI were infected with HCV genotype 3 and two of them (2/6) had a slight elevation of their liver enzymes. Conclusion: Seronegative OCI does exist among local hemodialysis patients, with normal or persistently abnormal liver enzyme values. Further investigation is needed to study the mode of viral transmission and clinical significance of OCI in HD setting. IIUM Press 2019-12 Article PeerReviewed application/pdf en http://irep.iium.edu.my/78061/1/Fazlin%20IMJM%202019.pdf Abdul Rahman, Siti Nurul Fazlin and Hamzah, Hairul Aini and Mustafa Mahmoud, Mohammed Imad Al-Deen (2019) Seronegative occult Hepatitis C virus infection (OCI) in a main haemodialysis centre In Pahang, Malaysia. IIUM Medical Journal Malaysia, 18 (3). pp. 11-19. E-ISSN 1823-4631 http://iiumedic.net/imjm/v1/download/volume_18_no_3/Vol18No3-011-019.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QR Microbiology QR355 Virology
spellingShingle	QR Microbiology QR355 Virology Abdul Rahman, Siti Nurul Fazlin Hamzah, Hairul Aini Mustafa Mahmoud, Mohammed Imad Al-Deen Seronegative occult Hepatitis C virus infection (OCI) in a main haemodialysis centre In Pahang, Malaysia
description	Introduction: Occult HCV infection has a predilection for specific populations such as haemodialysis (HD) patients. The exact natural course, epidemiology, pathogenesis and clinical importance of OCI are unknown. We investigated the existence of OCI among local patients undergoing routine HD at a referral hospital in Pahang, Malaysia. Methods: Serum and peripheral blood mononuclear cells (PMBCs) were collected from peripheral venous blood samples of seropositive (anti-HCV positive) and seronegative (anti-HCV negative) HD patients as well as healthy individuals (negative control group). Inclusion criteria for the seronegative patients included elevated liver enzymes. Both conventional PCR and strand-specific PCR were used to detect the viral RNA and to indicate active viral replication in PBMCs respectively. Direct DNA sequencing was done to confirm the viral HCV RNA and their genotypes. Results: In the majority (90-100%) of seropositive chronic hepatitis C patients, viral RNA was detected in both serum and PMBCs . Meanwhile, out of 22 seronegative patients, 6 (27%) showed active viral replication in PBMCs but no detectable viral RNA presence in the serum. None of the negative control group had detectable viral RNA. All seronegative patients with OCI were infected with HCV genotype 3 and two of them (2/6) had a slight elevation of their liver enzymes. Conclusion: Seronegative OCI does exist among local hemodialysis patients, with normal or persistently abnormal liver enzyme values. Further investigation is needed to study the mode of viral transmission and clinical significance of OCI in HD setting.
format	Article
author	Abdul Rahman, Siti Nurul Fazlin Hamzah, Hairul Aini Mustafa Mahmoud, Mohammed Imad Al-Deen
author_facet	Abdul Rahman, Siti Nurul Fazlin Hamzah, Hairul Aini Mustafa Mahmoud, Mohammed Imad Al-Deen
author_sort	Abdul Rahman, Siti Nurul Fazlin
title	Seronegative occult Hepatitis C virus infection (OCI) in a main haemodialysis centre In Pahang, Malaysia
title_short	Seronegative occult Hepatitis C virus infection (OCI) in a main haemodialysis centre In Pahang, Malaysia
title_full	Seronegative occult Hepatitis C virus infection (OCI) in a main haemodialysis centre In Pahang, Malaysia
title_fullStr	Seronegative occult Hepatitis C virus infection (OCI) in a main haemodialysis centre In Pahang, Malaysia
title_full_unstemmed	Seronegative occult Hepatitis C virus infection (OCI) in a main haemodialysis centre In Pahang, Malaysia
title_sort	seronegative occult hepatitis c virus infection (oci) in a main haemodialysis centre in pahang, malaysia
publisher	IIUM Press
publishDate	2019
url	http://irep.iium.edu.my/78061/ http://irep.iium.edu.my/78061/ http://irep.iium.edu.my/78061/1/Fazlin%20IMJM%202019.pdf
first_indexed	2023-09-18T21:50:01Z
last_indexed	2023-09-18T21:50:01Z
_version_	1777413706968203264

Seronegative occult Hepatitis C virus infection (OCI) in a main haemodialysis centre In Pahang, Malaysia

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