In vitro propagation of Etlingera elatior (Jack) (torch ginger)

An efficient protocol for complete plant regeneration from suckers of Etlingera elatior (Jack) has been developed. The addition of N6-benzyl amino-purine (BAP) (0, 13.32, 22.20, 31.08, and 44.40 μM) to the culture medium comprising Murashige and Skoog (MS) basal salts, 3% sucrose, 0.4% Gelrite™ did...

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Main Authors: Yunus, Muhamad Fahmi, Abd Aziz, Maheran, Abdul Kadir, Mihdzar, Abdul Rashid, Azmi
Format: Article
Language:English
Published: Elsevier 2012
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Online Access:http://irep.iium.edu.my/78030/
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http://irep.iium.edu.my/78030/1/In%20vitro%20propagation%20of%20Etlingera%20elatior%20%28Jack%29%20%28torch%20ginger%29.pdf
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spelling iium-780302020-02-18T01:09:18Z http://irep.iium.edu.my/78030/ In vitro propagation of Etlingera elatior (Jack) (torch ginger) Yunus, Muhamad Fahmi Abd Aziz, Maheran Abdul Kadir, Mihdzar Abdul Rashid, Azmi SB Plant culture An efficient protocol for complete plant regeneration from suckers of Etlingera elatior (Jack) has been developed. The addition of N6-benzyl amino-purine (BAP) (0, 13.32, 22.20, 31.08, and 44.40 μM) to the culture medium comprising Murashige and Skoog (MS) basal salts, 3% sucrose, 0.4% Gelrite™ did not show any significant effects on percentage of shoot induction and mean number of shoots produced. Various concentrations of BAP (0, 13.32, 22.20, 31.08, and 44.40 μM), 6-furfurylaminopurine (kinetin) (0, 13.95, 23.25, 32.55, and 46.50 μM) and N6-(2-isopentenyl) adenine (2-iP) (0, 14.76, 24.6, 34.44, and 49.2 μM) were tested for shoot multiplication. BAP at all levels were found suitable for the multiplication of shoot. However, the low level of 13.32 μM BAP was chosen as the best concentration of BAP due to economic feasibility. The best root proliferation was observed on MS medium without plant growth regulator (PGR). Assessment of various potting media for acclimatization showed medium containing soil:sand:peat moss (1:1:1) produced high survival of plantlets, number of leaves produced per plant and the plant height. This protocol provides a basis for future studies on mutation induction, genetic engineering, and could be applied for large-scale multiplication of this valuable plant. Elsevier 2012-02-24 Article PeerReviewed application/pdf en http://irep.iium.edu.my/78030/1/In%20vitro%20propagation%20of%20Etlingera%20elatior%20%28Jack%29%20%28torch%20ginger%29.pdf Yunus, Muhamad Fahmi and Abd Aziz, Maheran and Abdul Kadir, Mihdzar and Abdul Rashid, Azmi (2012) In vitro propagation of Etlingera elatior (Jack) (torch ginger). Scientia Horticulturae, 135. pp. 145-150. ISSN 0304-4238 https://www.sciencedirect.com/science/article/abs/pii/S0304423811006480 10.1016/j.scienta.2011.12.016
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic SB Plant culture
spellingShingle SB Plant culture
Yunus, Muhamad Fahmi
Abd Aziz, Maheran
Abdul Kadir, Mihdzar
Abdul Rashid, Azmi
In vitro propagation of Etlingera elatior (Jack) (torch ginger)
description An efficient protocol for complete plant regeneration from suckers of Etlingera elatior (Jack) has been developed. The addition of N6-benzyl amino-purine (BAP) (0, 13.32, 22.20, 31.08, and 44.40 μM) to the culture medium comprising Murashige and Skoog (MS) basal salts, 3% sucrose, 0.4% Gelrite™ did not show any significant effects on percentage of shoot induction and mean number of shoots produced. Various concentrations of BAP (0, 13.32, 22.20, 31.08, and 44.40 μM), 6-furfurylaminopurine (kinetin) (0, 13.95, 23.25, 32.55, and 46.50 μM) and N6-(2-isopentenyl) adenine (2-iP) (0, 14.76, 24.6, 34.44, and 49.2 μM) were tested for shoot multiplication. BAP at all levels were found suitable for the multiplication of shoot. However, the low level of 13.32 μM BAP was chosen as the best concentration of BAP due to economic feasibility. The best root proliferation was observed on MS medium without plant growth regulator (PGR). Assessment of various potting media for acclimatization showed medium containing soil:sand:peat moss (1:1:1) produced high survival of plantlets, number of leaves produced per plant and the plant height. This protocol provides a basis for future studies on mutation induction, genetic engineering, and could be applied for large-scale multiplication of this valuable plant.
format Article
author Yunus, Muhamad Fahmi
Abd Aziz, Maheran
Abdul Kadir, Mihdzar
Abdul Rashid, Azmi
author_facet Yunus, Muhamad Fahmi
Abd Aziz, Maheran
Abdul Kadir, Mihdzar
Abdul Rashid, Azmi
author_sort Yunus, Muhamad Fahmi
title In vitro propagation of Etlingera elatior (Jack) (torch ginger)
title_short In vitro propagation of Etlingera elatior (Jack) (torch ginger)
title_full In vitro propagation of Etlingera elatior (Jack) (torch ginger)
title_fullStr In vitro propagation of Etlingera elatior (Jack) (torch ginger)
title_full_unstemmed In vitro propagation of Etlingera elatior (Jack) (torch ginger)
title_sort in vitro propagation of etlingera elatior (jack) (torch ginger)
publisher Elsevier
publishDate 2012
url http://irep.iium.edu.my/78030/
http://irep.iium.edu.my/78030/
http://irep.iium.edu.my/78030/
http://irep.iium.edu.my/78030/1/In%20vitro%20propagation%20of%20Etlingera%20elatior%20%28Jack%29%20%28torch%20ginger%29.pdf
first_indexed 2023-09-18T21:49:59Z
last_indexed 2023-09-18T21:49:59Z
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