Structural changes and the differential expression of osteoprotegerin (OPG) and receptor activator of nuclear factor kB ligand (RANKL) in subchondral bone during the development of osteoarthritis

Osteoarthritis is a degenerative joint disease that is characterized by subchondral bone changes. These changes may be associated with bone remodelling, which involve coordinative functions between bone formation and resorption markers such as osteoprotegerin (OPG) and receptor activator of nuclear...

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Main Authors:	AKMAN, AKMA AZMIERA, Talib, Ain Aisyah, Md Yusof, Azirah, Khalid, Kamarul Ariffin, Yusof, Nurul Asyiqin, Abdul Ghani, Radiah, Hamdan, Asmah Hanim, Zamli, Zaitunnatakhin
Format:	Article
Language:	English
Published:	2018
Subjects:	RB Pathology
Online Access:	http://irep.iium.edu.my/77748/ http://irep.iium.edu.my/77748/1/STRUCTURAL%20CHANGES%20AND%20THE%20DIFFERENTIAL%20-%20Malaysia%20Applied%20Biology%202018.pdf

id	iium-77748
recordtype	eprints
spelling	iium-777482020-01-28T03:59:27Z http://irep.iium.edu.my/77748/ Structural changes and the differential expression of osteoprotegerin (OPG) and receptor activator of nuclear factor kB ligand (RANKL) in subchondral bone during the development of osteoarthritis AKMAN, AKMA AZMIERA Talib, Ain Aisyah Md Yusof, Azirah Khalid, Kamarul Ariffin Yusof, Nurul Asyiqin Abdul Ghani, Radiah Hamdan, Asmah Hanim Zamli, Zaitunnatakhin RB Pathology Osteoarthritis is a degenerative joint disease that is characterized by subchondral bone changes. These changes may be associated with bone remodelling, which involve coordinative functions between bone formation and resorption markers such as osteoprotegerin (OPG) and receptor activator of nuclear factor kB ligand (RANKL), respectively. Previous studies have shown that the structural changes were different between the subchondral bone plate and trabecular bone. However, the expression of bone formation and bone resorption markers underlying the differences between these two regions has yet to be determined. Hence, the present study aims to determine the structural changes and expressions of OPG/RANKL in the subchondral bone plate and trabecular bone during the development of osteoarthritis in Dunkin Hartley guinea pigs. Eighteen tibias were scanned using a micro-computed tomography at three different time points; 10, 20 and 30 weeks of age to determine their subchondral bone plate and trabecular bone thickness. Immunohistochemistry and histopathology methods were conducted to determine the expression of OPG/RANKL and microscopic osteoarthritis score, respectively. The results showed that subchondral bone plate and trabecular bone thickness were greater in the medial than the lateral side of the tibial plateau, and increased significantly with ageing (p < 0.01). In addition, across the time points, the OPG/RANKL ratio of medial Sbp initially increased, before decreasing at the final time point. In contrast, the OPG/RANKL ratio of medial Tb was initially constant but then decreased at 30 weeks of age. Interestingly, a significant correlation was observed between the subchondral bone plate thickness and OPG/RANKL ratio with microscopic osteoarthritis scores (r = 0.5, p < 0.05), suggesting that the subchondral bone plate plays an important role in the pathogenesis of osteoarthritis. 2018-12-31 Article PeerReviewed application/pdf en http://irep.iium.edu.my/77748/1/STRUCTURAL%20CHANGES%20AND%20THE%20DIFFERENTIAL%20-%20Malaysia%20Applied%20Biology%202018.pdf AKMAN, AKMA AZMIERA and Talib, Ain Aisyah and Md Yusof, Azirah and Khalid, Kamarul Ariffin and Yusof, Nurul Asyiqin and Abdul Ghani, Radiah and Hamdan, Asmah Hanim and Zamli, Zaitunnatakhin (2018) Structural changes and the differential expression of osteoprotegerin (OPG) and receptor activator of nuclear factor kB ligand (RANKL) in subchondral bone during the development of osteoarthritis. Malaysian Applied Biology. ISSN 0126-8643
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RB Pathology
spellingShingle	RB Pathology AKMAN, AKMA AZMIERA Talib, Ain Aisyah Md Yusof, Azirah Khalid, Kamarul Ariffin Yusof, Nurul Asyiqin Abdul Ghani, Radiah Hamdan, Asmah Hanim Zamli, Zaitunnatakhin Structural changes and the differential expression of osteoprotegerin (OPG) and receptor activator of nuclear factor kB ligand (RANKL) in subchondral bone during the development of osteoarthritis
description	Osteoarthritis is a degenerative joint disease that is characterized by subchondral bone changes. These changes may be associated with bone remodelling, which involve coordinative functions between bone formation and resorption markers such as osteoprotegerin (OPG) and receptor activator of nuclear factor kB ligand (RANKL), respectively. Previous studies have shown that the structural changes were different between the subchondral bone plate and trabecular bone. However, the expression of bone formation and bone resorption markers underlying the differences between these two regions has yet to be determined. Hence, the present study aims to determine the structural changes and expressions of OPG/RANKL in the subchondral bone plate and trabecular bone during the development of osteoarthritis in Dunkin Hartley guinea pigs. Eighteen tibias were scanned using a micro-computed tomography at three different time points; 10, 20 and 30 weeks of age to determine their subchondral bone plate and trabecular bone thickness. Immunohistochemistry and histopathology methods were conducted to determine the expression of OPG/RANKL and microscopic osteoarthritis score, respectively. The results showed that subchondral bone plate and trabecular bone thickness were greater in the medial than the lateral side of the tibial plateau, and increased significantly with ageing (p < 0.01). In addition, across the time points, the OPG/RANKL ratio of medial Sbp initially increased, before decreasing at the final time point. In contrast, the OPG/RANKL ratio of medial Tb was initially constant but then decreased at 30 weeks of age. Interestingly, a significant correlation was observed between the subchondral bone plate thickness and OPG/RANKL ratio with microscopic osteoarthritis scores (r = 0.5, p < 0.05), suggesting that the subchondral bone plate plays an important role in the pathogenesis of osteoarthritis.
format	Article
author	AKMAN, AKMA AZMIERA Talib, Ain Aisyah Md Yusof, Azirah Khalid, Kamarul Ariffin Yusof, Nurul Asyiqin Abdul Ghani, Radiah Hamdan, Asmah Hanim Zamli, Zaitunnatakhin
author_facet	AKMAN, AKMA AZMIERA Talib, Ain Aisyah Md Yusof, Azirah Khalid, Kamarul Ariffin Yusof, Nurul Asyiqin Abdul Ghani, Radiah Hamdan, Asmah Hanim Zamli, Zaitunnatakhin
author_sort	AKMAN, AKMA AZMIERA
title	Structural changes and the differential expression of osteoprotegerin (OPG) and receptor activator of nuclear factor kB ligand (RANKL) in subchondral bone during the development of osteoarthritis
title_short	Structural changes and the differential expression of osteoprotegerin (OPG) and receptor activator of nuclear factor kB ligand (RANKL) in subchondral bone during the development of osteoarthritis
title_full	Structural changes and the differential expression of osteoprotegerin (OPG) and receptor activator of nuclear factor kB ligand (RANKL) in subchondral bone during the development of osteoarthritis
title_fullStr	Structural changes and the differential expression of osteoprotegerin (OPG) and receptor activator of nuclear factor kB ligand (RANKL) in subchondral bone during the development of osteoarthritis
title_full_unstemmed	Structural changes and the differential expression of osteoprotegerin (OPG) and receptor activator of nuclear factor kB ligand (RANKL) in subchondral bone during the development of osteoarthritis
title_sort	structural changes and the differential expression of osteoprotegerin (opg) and receptor activator of nuclear factor kb ligand (rankl) in subchondral bone during the development of osteoarthritis
publishDate	2018
url	http://irep.iium.edu.my/77748/ http://irep.iium.edu.my/77748/1/STRUCTURAL%20CHANGES%20AND%20THE%20DIFFERENTIAL%20-%20Malaysia%20Applied%20Biology%202018.pdf
first_indexed	2023-09-18T21:49:38Z
last_indexed	2023-09-18T21:49:38Z
_version_	1777413682490245120

Structural changes and the differential expression of osteoprotegerin (OPG) and receptor activator of nuclear factor kB ligand (RANKL) in subchondral bone during the development of osteoarthritis

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