Measurement of pterygium tissue dry weight using two different tissue preparation techniques in freeze-dry method

Introduction: The proposed freeze-dried approach for estimating pterygium dry weight closes the gap which address the possible effect of corneal compression due to pterygium on the corneal surface, translating simple laboratory procedure to a clinical practice which essential in predicting induced-c...

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Main Authors:	Hilmi, Mohd Radzi, Mohd Kamal, Khairidzan, Che Azemin, Mohd Zulfaezal, Ariffin, Azrin Esmady
Format:	Article
Language:	English English
Published:	Universitas Islam Agung Sultan Semarang 2019
Subjects:	RE Ophthalmology
Online Access:	http://irep.iium.edu.my/77596/ http://irep.iium.edu.my/77596/1/Editor_Author%20Correspondence%20Sains%20Medika%20-%20Dry%20weight.pdf http://irep.iium.edu.my/77596/7/Measurement%20of%20Pterygium%20Tissue%20Dry%20Weight%20Using%20Two%20Different%20Tissue%20Preparation%20Techniques%20in%20Freeze-Dry%20Method.pdf

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recordtype	eprints
spelling	iium-775962020-01-28T05:20:03Z http://irep.iium.edu.my/77596/ Measurement of pterygium tissue dry weight using two different tissue preparation techniques in freeze-dry method Hilmi, Mohd Radzi Mohd Kamal, Khairidzan Che Azemin, Mohd Zulfaezal Ariffin, Azrin Esmady RE Ophthalmology Introduction: The proposed freeze-dried approach for estimating pterygium dry weight closes the gap which address the possible effect of corneal compression due to pterygium on the corneal surface, translating simple laboratory procedure to a clinical practice which essential in predicting induced-corneal astigmatism. This study aim to compare an objective method in quantifying pterygium dry weight utilizing freeze-dried method via two different tissue preparation methods. Methods: 60 primary pterygium were excised using controlled partial avulsion technique performed by a single surgeon (KMK), and divided into two groups: formalin-fixed (n=30) and non-fixed (n=30). Initially, each sterile container weight was measured and 5ml of 5% buffered formaldehyde were filled and stored for 1 week for formalin-fixed group while, 5ml distilled water were filled for non-fixed pterygium group. Each container was pre-frozen for 12 hours prior to freeze-dried (- 80 ºC for 24 hours). The final product known as net pterygium tissue mass (NPTM) then weighted as dry weight. Comparative analysis of wet and dry weight, and percentage of NPTM between groups were performed via independent t-test. Results: The overall mean and SD for formalin-fixed and non-fixed pterygium wet weight were 253.33 ± 82.17 μg and 255.17 ± 63.52 μg, and dry weight were 184.92 ± 84.31 μg and 179.54 ± 72.85 μg respectively. In terms of percentage of NPTM, formalin-fixed group revealed slightly higher percentage compared to non-fixed pterygium tissue with 69.39 ± 13.29 % and 67.75 ± 13.27 % respectively. The difference of pterygium dry weight, between formalin-fixed and non-fixed pterygium tissue were statistically insignificance (P = 0.792). Conclusions: Freeze-dried method can be used to quantify the NPTM (dry weight) of pterygium fibrovascular tissue. Both methods (formalin-fixed and non-fixed) are reliable in producing results. This is proven as there is no statistical significance between the two methods. Universitas Islam Agung Sultan Semarang 2019-07-15 Article PeerReviewed application/pdf en http://irep.iium.edu.my/77596/1/Editor_Author%20Correspondence%20Sains%20Medika%20-%20Dry%20weight.pdf application/pdf en http://irep.iium.edu.my/77596/7/Measurement%20of%20Pterygium%20Tissue%20Dry%20Weight%20Using%20Two%20Different%20Tissue%20Preparation%20Techniques%20in%20Freeze-Dry%20Method.pdf Hilmi, Mohd Radzi and Mohd Kamal, Khairidzan and Che Azemin, Mohd Zulfaezal and Ariffin, Azrin Esmady (2019) Measurement of pterygium tissue dry weight using two different tissue preparation techniques in freeze-dry method. Sains Medika. ISSN 2085-1545 E-ISSN 2339-093X (In Press)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	RE Ophthalmology
spellingShingle	RE Ophthalmology Hilmi, Mohd Radzi Mohd Kamal, Khairidzan Che Azemin, Mohd Zulfaezal Ariffin, Azrin Esmady Measurement of pterygium tissue dry weight using two different tissue preparation techniques in freeze-dry method
description	Introduction: The proposed freeze-dried approach for estimating pterygium dry weight closes the gap which address the possible effect of corneal compression due to pterygium on the corneal surface, translating simple laboratory procedure to a clinical practice which essential in predicting induced-corneal astigmatism. This study aim to compare an objective method in quantifying pterygium dry weight utilizing freeze-dried method via two different tissue preparation methods. Methods: 60 primary pterygium were excised using controlled partial avulsion technique performed by a single surgeon (KMK), and divided into two groups: formalin-fixed (n=30) and non-fixed (n=30). Initially, each sterile container weight was measured and 5ml of 5% buffered formaldehyde were filled and stored for 1 week for formalin-fixed group while, 5ml distilled water were filled for non-fixed pterygium group. Each container was pre-frozen for 12 hours prior to freeze-dried (- 80 ºC for 24 hours). The final product known as net pterygium tissue mass (NPTM) then weighted as dry weight. Comparative analysis of wet and dry weight, and percentage of NPTM between groups were performed via independent t-test. Results: The overall mean and SD for formalin-fixed and non-fixed pterygium wet weight were 253.33 ± 82.17 μg and 255.17 ± 63.52 μg, and dry weight were 184.92 ± 84.31 μg and 179.54 ± 72.85 μg respectively. In terms of percentage of NPTM, formalin-fixed group revealed slightly higher percentage compared to non-fixed pterygium tissue with 69.39 ± 13.29 % and 67.75 ± 13.27 % respectively. The difference of pterygium dry weight, between formalin-fixed and non-fixed pterygium tissue were statistically insignificance (P = 0.792). Conclusions: Freeze-dried method can be used to quantify the NPTM (dry weight) of pterygium fibrovascular tissue. Both methods (formalin-fixed and non-fixed) are reliable in producing results. This is proven as there is no statistical significance between the two methods.
format	Article
author	Hilmi, Mohd Radzi Mohd Kamal, Khairidzan Che Azemin, Mohd Zulfaezal Ariffin, Azrin Esmady
author_facet	Hilmi, Mohd Radzi Mohd Kamal, Khairidzan Che Azemin, Mohd Zulfaezal Ariffin, Azrin Esmady
author_sort	Hilmi, Mohd Radzi
title	Measurement of pterygium tissue dry weight using two different tissue preparation techniques in freeze-dry method
title_short	Measurement of pterygium tissue dry weight using two different tissue preparation techniques in freeze-dry method
title_full	Measurement of pterygium tissue dry weight using two different tissue preparation techniques in freeze-dry method
title_fullStr	Measurement of pterygium tissue dry weight using two different tissue preparation techniques in freeze-dry method
title_full_unstemmed	Measurement of pterygium tissue dry weight using two different tissue preparation techniques in freeze-dry method
title_sort	measurement of pterygium tissue dry weight using two different tissue preparation techniques in freeze-dry method
publisher	Universitas Islam Agung Sultan Semarang
publishDate	2019
url	http://irep.iium.edu.my/77596/ http://irep.iium.edu.my/77596/1/Editor_Author%20Correspondence%20Sains%20Medika%20-%20Dry%20weight.pdf http://irep.iium.edu.my/77596/7/Measurement%20of%20Pterygium%20Tissue%20Dry%20Weight%20Using%20Two%20Different%20Tissue%20Preparation%20Techniques%20in%20Freeze-Dry%20Method.pdf
first_indexed	2023-09-18T21:49:26Z
last_indexed	2023-09-18T21:49:26Z
_version_	1777413670139068416

Measurement of pterygium tissue dry weight using two different tissue preparation techniques in freeze-dry method

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