Cytotoxicity study of Basella alba leaves extract on brine shrimp lethality bioassay and mtt assay

Cytotoxicity study of Basella alba leaves extract on brine shrimp lethality bioassay and mtt assay

Introduction: The main objective of the present study is to investigate the cytotoxicity of crude extracts of Basella alba L. leaves. It has been reported to show various bioactivities, but the toxicity and cytotoxicity effects have not been conducted yet. Materials and Methods: The phytochemical sc...

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Main Authors: Azad, Abul Kalam, Islam, Mohd. Aminul, Sunzida, Nushrat Khan
Format: Article
Language:English
English
Published: Società Italo-Latinoamericana di Etnomedicina ( SILAE) 2019
Subjects:
RA1190 Toxicology
Online Access:http://irep.iium.edu.my/77493/
http://irep.iium.edu.my/77493/
http://irep.iium.edu.my/77493/7/77493%20CYTOTOXICITY%20STUDY.pdf
http://irep.iium.edu.my/77493/8/77493%20CYTOTOXICITY%20STUDY%20SCOPUS.pdf
id iium-77493
recordtype eprints
spelling iium-774932020-01-10T03:37:40Z http://irep.iium.edu.my/77493/ Cytotoxicity study of Basella alba leaves extract on brine shrimp lethality bioassay and mtt assay Azad, Abul Kalam Islam, Mohd. Aminul Sunzida, Nushrat Khan RA1190 Toxicology Introduction: The main objective of the present study is to investigate the cytotoxicity of crude extracts of Basella alba L. leaves. It has been reported to show various bioactivities, but the toxicity and cytotoxicity effects have not been conducted yet. Materials and Methods: The phytochemical screening of the plants extract has been carried qualitatively. The cytotoxic effect was determined through in vitro MTT assay using MCF-7 cell line, and brine shrimp lethality bioassay. Results: Brine shrimp lethality bioassay showed the percentage of survival rate of nauplii which were 72, 88, 88 and 96 % at the concentration of 200, 100, 50 and 25 μL/mL respectively. The mortality of brine shrimp nauplii was from 44% at the concentration of 800 μL/mL, while the negative control (Aluminium chloride) showed 80% mortality at the concentration of 20 μL/mL. However, the IC50 was found with the concentration of 617.31 μL/mL. In MTT assay, more than 90% cell viability showed from the concentration of 31.25 μL/mL to 250 μL/mL, while the vehicle control (DMSO; 1% v/v) was 63.71%. Moreover, the cytotoxicity assay on MCF-7 cell line also concluded that the extract of Basella alba L. leaves is nontoxic even with very high concentration as well. Conclusion: Since it is a popular vegetable which is consumed by the people of Asia every day, therefore, it was very important to study the toxicity of this plant. It has been clearly proven by this study that this vegetable has no toxic effect. Società Italo-Latinoamericana di Etnomedicina ( SILAE) 2019-08 Article PeerReviewed application/pdf en http://irep.iium.edu.my/77493/7/77493%20CYTOTOXICITY%20STUDY.pdf application/pdf en http://irep.iium.edu.my/77493/8/77493%20CYTOTOXICITY%20STUDY%20SCOPUS.pdf Azad, Abul Kalam and Islam, Mohd. Aminul and Sunzida, Nushrat Khan (2019) Cytotoxicity study of Basella alba leaves extract on brine shrimp lethality bioassay and mtt assay. Pharmacologyonline, 2. pp. 87-93. ISSN 1827-8620 https://pharmacologyonline.silae.it/files/archives/2019/vol2/PhOL_2019_2_A009_Azad.pdf
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
English
topic RA1190 Toxicology
spellingShingle RA1190 Toxicology
Azad, Abul Kalam
Islam, Mohd. Aminul
Sunzida, Nushrat Khan
Cytotoxicity study of Basella alba leaves extract on brine shrimp lethality bioassay and mtt assay
description Introduction: The main objective of the present study is to investigate the cytotoxicity of crude extracts of Basella alba L. leaves. It has been reported to show various bioactivities, but the toxicity and cytotoxicity effects have not been conducted yet. Materials and Methods: The phytochemical screening of the plants extract has been carried qualitatively. The cytotoxic effect was determined through in vitro MTT assay using MCF-7 cell line, and brine shrimp lethality bioassay. Results: Brine shrimp lethality bioassay showed the percentage of survival rate of nauplii which were 72, 88, 88 and 96 % at the concentration of 200, 100, 50 and 25 μL/mL respectively. The mortality of brine shrimp nauplii was from 44% at the concentration of 800 μL/mL, while the negative control (Aluminium chloride) showed 80% mortality at the concentration of 20 μL/mL. However, the IC50 was found with the concentration of 617.31 μL/mL. In MTT assay, more than 90% cell viability showed from the concentration of 31.25 μL/mL to 250 μL/mL, while the vehicle control (DMSO; 1% v/v) was 63.71%. Moreover, the cytotoxicity assay on MCF-7 cell line also concluded that the extract of Basella alba L. leaves is nontoxic even with very high concentration as well. Conclusion: Since it is a popular vegetable which is consumed by the people of Asia every day, therefore, it was very important to study the toxicity of this plant. It has been clearly proven by this study that this vegetable has no toxic effect.
format Article
author Azad, Abul Kalam
Islam, Mohd. Aminul
Sunzida, Nushrat Khan
author_facet Azad, Abul Kalam
Islam, Mohd. Aminul
Sunzida, Nushrat Khan
author_sort Azad, Abul Kalam
title Cytotoxicity study of Basella alba leaves extract on brine shrimp lethality bioassay and mtt assay
title_short Cytotoxicity study of Basella alba leaves extract on brine shrimp lethality bioassay and mtt assay
title_full Cytotoxicity study of Basella alba leaves extract on brine shrimp lethality bioassay and mtt assay
title_fullStr Cytotoxicity study of Basella alba leaves extract on brine shrimp lethality bioassay and mtt assay
title_full_unstemmed Cytotoxicity study of Basella alba leaves extract on brine shrimp lethality bioassay and mtt assay
title_sort cytotoxicity study of basella alba leaves extract on brine shrimp lethality bioassay and mtt assay
publisher Società Italo-Latinoamericana di Etnomedicina ( SILAE)
publishDate 2019
url http://irep.iium.edu.my/77493/
http://irep.iium.edu.my/77493/
http://irep.iium.edu.my/77493/7/77493%20CYTOTOXICITY%20STUDY.pdf
http://irep.iium.edu.my/77493/8/77493%20CYTOTOXICITY%20STUDY%20SCOPUS.pdf
first_indexed 2023-09-18T21:49:18Z
last_indexed 2023-09-18T21:49:18Z
_version_ 1777413661202055168

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