Plant tissue culture as a tool for sustainable production of bentong ginger (Zingiber officinale var. Bentong) plantlets

Bentong ginger (Zingiber officinale var. Bentong) is an exotic ginger variety that can only be grown at highlands of Bentong district, Pahang, due to the fertile soil and cold temperate climate. Morphologically it has larger rhizome, thinner skin and contains less fibrous pulp compared to the domes...

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Main Authors:	M. Sundram, Tamil Chelvan, Khusairi, Ayadi M., Zulkifli, Mazidah, Yunus, Muhamad Fahmi, Zainuddin, Zarina
Format:	Conference or Workshop Item
Language:	English English
Published:	2019
Subjects:	QH301 Biology
Online Access:	http://irep.iium.edu.my/77349/ http://irep.iium.edu.my/77349/2/77349%20abstract.pdf http://irep.iium.edu.my/77349/3/77349%20poster.pdf

id	iium-77349
recordtype	eprints
spelling	iium-773492020-02-03T07:17:05Z http://irep.iium.edu.my/77349/ Plant tissue culture as a tool for sustainable production of bentong ginger (Zingiber officinale var. Bentong) plantlets M. Sundram, Tamil Chelvan Khusairi, Ayadi M. Zulkifli, Mazidah Yunus, Muhamad Fahmi Zainuddin, Zarina QH301 Biology Bentong ginger (Zingiber officinale var. Bentong) is an exotic ginger variety that can only be grown at highlands of Bentong district, Pahang, due to the fertile soil and cold temperate climate. Morphologically it has larger rhizome, thinner skin and contains less fibrous pulp compared to the domestic gingers. Besides, bentong ginger also has higher content of pharmaceutically important compounds (i.e. gingerol and shogaol) compared to other varieties of Malaysian gingers. However, cultivating this ginger through conventional agricultural practice has several constraints. Every season, almost 40% of the harvested rhizomes are set aside to be used as seeds for next cycle of cultivation. This has been a major drawback, hindering the continuous supply of ginger to fulfil the increasing market demand. Thus, in this final year project (FYP) research a systematic approach was taken to establish in vitro cultures of Bentong ginger plantlets. Prior to introduction into Murashige and Skoog (MS) media, young rhizome shoot bud explants were surface sterilised using different sterilization methods. Various responses were obtained from shoot bud explants cultured on MS basal medium supplemented with different concentrations of BAP and NAA; either alone or in combinations. Cultures were maintained under light condition 16 h/day photoperiod from white light of fluorescent lamps. In this preliminary study, augmentation of MS-medium with 3 mg/l BAP and with 1 mg/l NAA recorded the highest number of shootlets and roots multiplication. This in vitro regenerated Bentong ginger plantlets could serve the increasing needs of the planting materials for smallholder farmers. Indirectly, this could also improve the economic wellbeing of local farmers. 2019 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/77349/2/77349%20abstract.pdf application/pdf en http://irep.iium.edu.my/77349/3/77349%20poster.pdf M. Sundram, Tamil Chelvan and Khusairi, Ayadi M. and Zulkifli, Mazidah and Yunus, Muhamad Fahmi and Zainuddin, Zarina (2019) Plant tissue culture as a tool for sustainable production of bentong ginger (Zingiber officinale var. Bentong) plantlets. In: Kuantan Research Day 2019, 23rd October 2019, Kuantan, Pahang. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	QH301 Biology
spellingShingle	QH301 Biology M. Sundram, Tamil Chelvan Khusairi, Ayadi M. Zulkifli, Mazidah Yunus, Muhamad Fahmi Zainuddin, Zarina Plant tissue culture as a tool for sustainable production of bentong ginger (Zingiber officinale var. Bentong) plantlets
description	Bentong ginger (Zingiber officinale var. Bentong) is an exotic ginger variety that can only be grown at highlands of Bentong district, Pahang, due to the fertile soil and cold temperate climate. Morphologically it has larger rhizome, thinner skin and contains less fibrous pulp compared to the domestic gingers. Besides, bentong ginger also has higher content of pharmaceutically important compounds (i.e. gingerol and shogaol) compared to other varieties of Malaysian gingers. However, cultivating this ginger through conventional agricultural practice has several constraints. Every season, almost 40% of the harvested rhizomes are set aside to be used as seeds for next cycle of cultivation. This has been a major drawback, hindering the continuous supply of ginger to fulfil the increasing market demand. Thus, in this final year project (FYP) research a systematic approach was taken to establish in vitro cultures of Bentong ginger plantlets. Prior to introduction into Murashige and Skoog (MS) media, young rhizome shoot bud explants were surface sterilised using different sterilization methods. Various responses were obtained from shoot bud explants cultured on MS basal medium supplemented with different concentrations of BAP and NAA; either alone or in combinations. Cultures were maintained under light condition 16 h/day photoperiod from white light of fluorescent lamps. In this preliminary study, augmentation of MS-medium with 3 mg/l BAP and with 1 mg/l NAA recorded the highest number of shootlets and roots multiplication. This in vitro regenerated Bentong ginger plantlets could serve the increasing needs of the planting materials for smallholder farmers. Indirectly, this could also improve the economic wellbeing of local farmers.
format	Conference or Workshop Item
author	M. Sundram, Tamil Chelvan Khusairi, Ayadi M. Zulkifli, Mazidah Yunus, Muhamad Fahmi Zainuddin, Zarina
author_facet	M. Sundram, Tamil Chelvan Khusairi, Ayadi M. Zulkifli, Mazidah Yunus, Muhamad Fahmi Zainuddin, Zarina
author_sort	M. Sundram, Tamil Chelvan
title	Plant tissue culture as a tool for sustainable production of bentong ginger (Zingiber officinale var. Bentong) plantlets
title_short	Plant tissue culture as a tool for sustainable production of bentong ginger (Zingiber officinale var. Bentong) plantlets
title_full	Plant tissue culture as a tool for sustainable production of bentong ginger (Zingiber officinale var. Bentong) plantlets
title_fullStr	Plant tissue culture as a tool for sustainable production of bentong ginger (Zingiber officinale var. Bentong) plantlets
title_full_unstemmed	Plant tissue culture as a tool for sustainable production of bentong ginger (Zingiber officinale var. Bentong) plantlets
title_sort	plant tissue culture as a tool for sustainable production of bentong ginger (zingiber officinale var. bentong) plantlets
publishDate	2019
url	http://irep.iium.edu.my/77349/ http://irep.iium.edu.my/77349/2/77349%20abstract.pdf http://irep.iium.edu.my/77349/3/77349%20poster.pdf
first_indexed	2023-09-18T21:49:05Z
last_indexed	2023-09-18T21:49:05Z
_version_	1777413648012017664

Plant tissue culture as a tool for sustainable production of bentong ginger (Zingiber officinale var. Bentong) plantlets

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