Comparison of the in vitro and in vivo toxic effects of thymoquinone using oral cancer HSC-3 and HSC-4 cell lines, oral fibroblasts, HACAT cell line, and Zebrafish embryos

The discovery and development of anticancer agents were made through long processes from screening new compounds in various cell lines, animal experimental studies, until clinical trials Majority of cancer chemotherapeutic agents have been initially screened in an in vitro cell-based system and the...

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Main Authors:	How, Fiona Ni Foong, Solachuddin Jauhari, Arief Ichwan, Kasmuri, Abdul Razak
Format:	Conference or Workshop Item
Language:	English English
Published:	Elsevier 2019
Subjects:	QD Chemistry RK Dentistry
Online Access:	http://irep.iium.edu.my/77267/ http://irep.iium.edu.my/77267/ http://irep.iium.edu.my/77267/1/Materials%20Today%20Proceedings%2016%20%282019%29%202108%E2%80%932114.pdf http://irep.iium.edu.my/77267/7/77267_Comparison%20of%20the%20in%20vitro%20and%20in%20vivo%20toxic%20effects_scopus.pdf

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spelling	iium-772672020-01-04T18:06:45Z http://irep.iium.edu.my/77267/ Comparison of the in vitro and in vivo toxic effects of thymoquinone using oral cancer HSC-3 and HSC-4 cell lines, oral fibroblasts, HACAT cell line, and Zebrafish embryos How, Fiona Ni Foong Solachuddin Jauhari, Arief Ichwan Kasmuri, Abdul Razak QD Chemistry RK Dentistry The discovery and development of anticancer agents were made through long processes from screening new compounds in various cell lines, animal experimental studies, until clinical trials Majority of cancer chemotherapeutic agents have been initially screened in an in vitro cell-based system and then subsequently studied in an in vivo model using small animals destined for pharmacokinetic properties. Zebrafish (Danio rerio) has become one of the most popular animal model systems used for chemical toxicity tests and drug discovery. Thymoquinone (TQ) is the main constituent of black seed (Nigella sativa, spp) essential oil which shows anti-neoplastic activities in various tumor cells. However, to date, studies on the toxicity of TQ on normal cells or animal model are still insufficient. Here we report that TQ is selective toxic to different human cell lines and show negligible effect on the Zebrafish. The MTT assay demonstrated that TQ induced cytotoxicity on oral cancer cells (HSC-3 and HSC-4) but not on the normal cells (human oral fibroblast and HACAT cells). Moreover, no significant morphological change was observed in the Zebrafish model following TQ exposure at the concentration (0.003 mg/mL), similar to those in the cancer cells. The study suggests that TQ as a cancer chemotherapeutic candidate without apparent adverse effects. Elsevier 2019-04-01 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/77267/1/Materials%20Today%20Proceedings%2016%20%282019%29%202108%E2%80%932114.pdf application/pdf en http://irep.iium.edu.my/77267/7/77267_Comparison%20of%20the%20in%20vitro%20and%20in%20vivo%20toxic%20effects_scopus.pdf How, Fiona Ni Foong and Solachuddin Jauhari, Arief Ichwan and Kasmuri, Abdul Razak (2019) Comparison of the in vitro and in vivo toxic effects of thymoquinone using oral cancer HSC-3 and HSC-4 cell lines, oral fibroblasts, HACAT cell line, and Zebrafish embryos. In: 2017 Conference on Biomedical and Advanced Materials, Bio-CAM 2017, 28-29 Nov 2017, Langkawi Island. https://www.journals.elsevier.com/materials-today-proceedings
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	QD Chemistry RK Dentistry
spellingShingle	QD Chemistry RK Dentistry How, Fiona Ni Foong Solachuddin Jauhari, Arief Ichwan Kasmuri, Abdul Razak Comparison of the in vitro and in vivo toxic effects of thymoquinone using oral cancer HSC-3 and HSC-4 cell lines, oral fibroblasts, HACAT cell line, and Zebrafish embryos
description	The discovery and development of anticancer agents were made through long processes from screening new compounds in various cell lines, animal experimental studies, until clinical trials Majority of cancer chemotherapeutic agents have been initially screened in an in vitro cell-based system and then subsequently studied in an in vivo model using small animals destined for pharmacokinetic properties. Zebrafish (Danio rerio) has become one of the most popular animal model systems used for chemical toxicity tests and drug discovery. Thymoquinone (TQ) is the main constituent of black seed (Nigella sativa, spp) essential oil which shows anti-neoplastic activities in various tumor cells. However, to date, studies on the toxicity of TQ on normal cells or animal model are still insufficient. Here we report that TQ is selective toxic to different human cell lines and show negligible effect on the Zebrafish. The MTT assay demonstrated that TQ induced cytotoxicity on oral cancer cells (HSC-3 and HSC-4) but not on the normal cells (human oral fibroblast and HACAT cells). Moreover, no significant morphological change was observed in the Zebrafish model following TQ exposure at the concentration (0.003 mg/mL), similar to those in the cancer cells. The study suggests that TQ as a cancer chemotherapeutic candidate without apparent adverse effects.
format	Conference or Workshop Item
author	How, Fiona Ni Foong Solachuddin Jauhari, Arief Ichwan Kasmuri, Abdul Razak
author_facet	How, Fiona Ni Foong Solachuddin Jauhari, Arief Ichwan Kasmuri, Abdul Razak
author_sort	How, Fiona Ni Foong
title	Comparison of the in vitro and in vivo toxic effects of thymoquinone using oral cancer HSC-3 and HSC-4 cell lines, oral fibroblasts, HACAT cell line, and Zebrafish embryos
title_short	Comparison of the in vitro and in vivo toxic effects of thymoquinone using oral cancer HSC-3 and HSC-4 cell lines, oral fibroblasts, HACAT cell line, and Zebrafish embryos
title_full	Comparison of the in vitro and in vivo toxic effects of thymoquinone using oral cancer HSC-3 and HSC-4 cell lines, oral fibroblasts, HACAT cell line, and Zebrafish embryos
title_fullStr	Comparison of the in vitro and in vivo toxic effects of thymoquinone using oral cancer HSC-3 and HSC-4 cell lines, oral fibroblasts, HACAT cell line, and Zebrafish embryos
title_full_unstemmed	Comparison of the in vitro and in vivo toxic effects of thymoquinone using oral cancer HSC-3 and HSC-4 cell lines, oral fibroblasts, HACAT cell line, and Zebrafish embryos
title_sort	comparison of the in vitro and in vivo toxic effects of thymoquinone using oral cancer hsc-3 and hsc-4 cell lines, oral fibroblasts, hacat cell line, and zebrafish embryos
publisher	Elsevier
publishDate	2019
url	http://irep.iium.edu.my/77267/ http://irep.iium.edu.my/77267/ http://irep.iium.edu.my/77267/1/Materials%20Today%20Proceedings%2016%20%282019%29%202108%E2%80%932114.pdf http://irep.iium.edu.my/77267/7/77267_Comparison%20of%20the%20in%20vitro%20and%20in%20vivo%20toxic%20effects_scopus.pdf
first_indexed	2023-09-18T21:48:59Z
last_indexed	2023-09-18T21:48:59Z
_version_	1777413641966977024

Comparison of the in vitro and in vivo toxic effects of thymoquinone using oral cancer HSC-3 and HSC-4 cell lines, oral fibroblasts, HACAT cell line, and Zebrafish embryos

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