GCMS-based profiling revealed bioactive compounds from Aquilaria malaccensis leaf extract
Bioactive compounds from plants have been shown to possess therapeutic values which are being used as medicine as well as ingredients for functional foods and nutraceuticals. While qualitative and quantitative studies on the bioactive compounds of plant extract are important to establish their cla...
| Main Authors: | , , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
2019
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/77253/ http://irep.iium.edu.my/77253/7/77253%20GCMS-based%20Profiling%20Revealed.pdf |
| Summary: | Bioactive compounds from plants have been shown to possess therapeutic values which are being used as
medicine as well as ingredients for functional foods and nutraceuticals. While qualitative and quantitative studies on
the bioactive compounds of plant extract are important to establish their claimed therapeutic properties; less is
focused on effects and interrelationship of process (extraction) conditions used to obtain the desired bioactive
compounds. As such, this study intends to determine the effects of different parameters on the extraction of
Aquilaria malaccensis leaf extract (ALEX) bioactive compounds. Metabolite profiling of bioactive compounds of
ALEX samples were conducted using gas chromatography-mass spectrometry (GCMS) with subsequent
multivariate statistical analysis through principal component analysis (PCA) with the aid of MATLAB software. The
GCMS analysis detected diverse potential bioactive compounds in ALEX samples with hydrocarbon, terpenes and
esters being the primary groups. n-hexadecanoic acid and hexadecanoic acid, 2-hydroxy-1-(hydroxymethyl)ethyl
ester were found to be present in most of the ALEX samples. PCA showed that same Soxhlet extraction parameters
were able to cluster the samples together and separate samples extracted with different extraction parameters
based on the relative abundance of the particular compounds. In conclusion, it is notable that the samples were
significantly separated based on Soxhlet extraction time as compared to the solid to solvent ratio.The findings from
this study would facilitate in extraction and purification of the desired bioactive compounds from agarwood leaf that
could be further used in development of functional foods and nutraceuticals. |
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