GC-MS analysis and antibacterial activity of Aquilaria malaccensis leaf extract

Ethnopharmacological practices have shown significant evidences of agarwood material as having myriad of pharmacological activities including anticancer, antipyretic and antioxidant. The complexity and variability of the chemical constitution of the plant establishes the quality of the medicinal...

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Main Authors:	Mohd Noor, Najihah, Hashim, Yumi Zuhanis Has-Yun, Mohammad Tormizi, Ummu Athiyah, Mohd Nasir, Asmak Muti;ah, Zainudin, Nur Umisha
Format:	Conference or Workshop Item
Language:	English
Published:	2019
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/77251/ http://irep.iium.edu.my/77251/ http://irep.iium.edu.my/77251/6/77251%20GC-MS%20analysis.pdf

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recordtype	eprints
spelling	iium-772512020-01-24T01:00:48Z http://irep.iium.edu.my/77251/ GC-MS analysis and antibacterial activity of Aquilaria malaccensis leaf extract Mohd Noor, Najihah Hashim, Yumi Zuhanis Has-Yun Mohammad Tormizi, Ummu Athiyah Mohd Nasir, Asmak Muti;ah Zainudin, Nur Umisha Q Science (General) Ethnopharmacological practices have shown significant evidences of agarwood material as having myriad of pharmacological activities including anticancer, antipyretic and antioxidant. The complexity and variability of the chemical constitution of the plant establishes the quality of the medicinal products. The present study was carried out to identify the phytochemical constituents of both derivatized and non-derivatized Aquilaria malaccensisleaf ethanolic extract (ALEX) and to study the anti-bacterial activity of ALEX extracted using Soxhlet extraction method. The metabolite profiling of bioactive compounds of ALEX samples were conducted using gas chromatography - mass spectrometry (GC-MS) coupled with the principal component analysis (PCA). The screening on the bacterial activity of the extract was determined by using modified Kirby Bauer disc diffusion method against leading bacterial pathogens: Staphylococcus aureus, Escherichia coli, Bacillus subtilis and Vibrio Vulnificus.GC-MS analysis of ALEX reveals a total of 127 compounds from the derivatized sample and only 22 compounds in the non-derivatized sample. Several biological active compoundsthat werefound in ALEX samples include hexadecanoic acid, trimethyl ester, hexadecenoic acid, ethyl ester, 2(4H)- Benzofuranone, 5,6,7,7a-tetrahydro-4,4,7a- trimethyl-, trans-9-Octadecenoic acid, trimethylsilyl ester and 4-Isopropylphenol, trimethylsilyl ether. ALEX showed comparable anti-bacterial activity with zone of inhibition of 6.0 - 15.7 mmfor gram positive bacteria and 2.0 - 13.0 mm for gram negative bacteria against control which is Dimethyl sulfoxide (DMSO). Penicillin was used as positive controlexhibited strong zone of inhibition of 21.0 – 28.3 mm. The antibacterial activity results indicate that ALEXpossessed mild to stronginhibitory activities against the pathogenic bacteria due to presence of various phytochemical constituents which can be used as source of drugs to fight infections caused by susceptible bacteria. 2019 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/77251/6/77251%20GC-MS%20analysis.pdf Mohd Noor, Najihah and Hashim, Yumi Zuhanis Has-Yun and Mohammad Tormizi, Ummu Athiyah and Mohd Nasir, Asmak Muti;ah and Zainudin, Nur Umisha (2019) GC-MS analysis and antibacterial activity of Aquilaria malaccensis leaf extract. In: 1st International Symposium of Agarwood and Aromatic Plants 2019 (1st ISAAP 2019), 1st-2nd October 2019, Lombok, Indonesia. (Unpublished) https://isaapp2019.unram.ac.id/wp-content/uploads/2019/10/NEW-Program-Book-5-Oct.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Mohd Noor, Najihah Hashim, Yumi Zuhanis Has-Yun Mohammad Tormizi, Ummu Athiyah Mohd Nasir, Asmak Muti;ah Zainudin, Nur Umisha GC-MS analysis and antibacterial activity of Aquilaria malaccensis leaf extract
description	Ethnopharmacological practices have shown significant evidences of agarwood material as having myriad of pharmacological activities including anticancer, antipyretic and antioxidant. The complexity and variability of the chemical constitution of the plant establishes the quality of the medicinal products. The present study was carried out to identify the phytochemical constituents of both derivatized and non-derivatized Aquilaria malaccensisleaf ethanolic extract (ALEX) and to study the anti-bacterial activity of ALEX extracted using Soxhlet extraction method. The metabolite profiling of bioactive compounds of ALEX samples were conducted using gas chromatography - mass spectrometry (GC-MS) coupled with the principal component analysis (PCA). The screening on the bacterial activity of the extract was determined by using modified Kirby Bauer disc diffusion method against leading bacterial pathogens: Staphylococcus aureus, Escherichia coli, Bacillus subtilis and Vibrio Vulnificus.GC-MS analysis of ALEX reveals a total of 127 compounds from the derivatized sample and only 22 compounds in the non-derivatized sample. Several biological active compoundsthat werefound in ALEX samples include hexadecanoic acid, trimethyl ester, hexadecenoic acid, ethyl ester, 2(4H)- Benzofuranone, 5,6,7,7a-tetrahydro-4,4,7a- trimethyl-, trans-9-Octadecenoic acid, trimethylsilyl ester and 4-Isopropylphenol, trimethylsilyl ether. ALEX showed comparable anti-bacterial activity with zone of inhibition of 6.0 - 15.7 mmfor gram positive bacteria and 2.0 - 13.0 mm for gram negative bacteria against control which is Dimethyl sulfoxide (DMSO). Penicillin was used as positive controlexhibited strong zone of inhibition of 21.0 – 28.3 mm. The antibacterial activity results indicate that ALEXpossessed mild to stronginhibitory activities against the pathogenic bacteria due to presence of various phytochemical constituents which can be used as source of drugs to fight infections caused by susceptible bacteria.
format	Conference or Workshop Item
author	Mohd Noor, Najihah Hashim, Yumi Zuhanis Has-Yun Mohammad Tormizi, Ummu Athiyah Mohd Nasir, Asmak Muti;ah Zainudin, Nur Umisha
author_facet	Mohd Noor, Najihah Hashim, Yumi Zuhanis Has-Yun Mohammad Tormizi, Ummu Athiyah Mohd Nasir, Asmak Muti;ah Zainudin, Nur Umisha
author_sort	Mohd Noor, Najihah
title	GC-MS analysis and antibacterial activity of Aquilaria malaccensis leaf extract
title_short	GC-MS analysis and antibacterial activity of Aquilaria malaccensis leaf extract
title_full	GC-MS analysis and antibacterial activity of Aquilaria malaccensis leaf extract
title_fullStr	GC-MS analysis and antibacterial activity of Aquilaria malaccensis leaf extract
title_full_unstemmed	GC-MS analysis and antibacterial activity of Aquilaria malaccensis leaf extract
title_sort	gc-ms analysis and antibacterial activity of aquilaria malaccensis leaf extract
publishDate	2019
url	http://irep.iium.edu.my/77251/ http://irep.iium.edu.my/77251/ http://irep.iium.edu.my/77251/6/77251%20GC-MS%20analysis.pdf
first_indexed	2023-09-18T21:48:58Z
last_indexed	2023-09-18T21:48:58Z
_version_	1777413640198029312

GC-MS analysis and antibacterial activity of Aquilaria malaccensis leaf extract

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