GC-MS analysis and antibacterial activity of Aquilaria malaccensis leaf extract
Ethnopharmacological practices have shown significant evidences of agarwood material as having myriad of pharmacological activities including anticancer, antipyretic and antioxidant. The complexity and variability of the chemical constitution of the plant establishes the quality of the medicinal...
| Main Authors: | , , , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
2019
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/77251/ http://irep.iium.edu.my/77251/ http://irep.iium.edu.my/77251/6/77251%20GC-MS%20analysis.pdf |
| Summary: | Ethnopharmacological practices have shown significant evidences of agarwood material
as having myriad of pharmacological activities including anticancer, antipyretic and antioxidant. The
complexity and variability of the chemical constitution of the plant establishes the quality of the
medicinal products. The present study was carried out to identify the phytochemical constituents of both
derivatized and non-derivatized Aquilaria malaccensisleaf ethanolic extract (ALEX) and to study the
anti-bacterial activity of ALEX extracted using Soxhlet extraction method. The metabolite profiling of
bioactive compounds of ALEX samples were conducted using gas chromatography - mass spectrometry
(GC-MS) coupled with the principal component analysis (PCA). The screening on the bacterial activity
of the extract was determined by using modified Kirby Bauer disc diffusion method against leading
bacterial pathogens: Staphylococcus aureus, Escherichia coli, Bacillus subtilis and Vibrio
Vulnificus.GC-MS analysis of ALEX reveals a total of 127 compounds from the derivatized sample and
only 22 compounds in the non-derivatized sample. Several biological active compoundsthat werefound
in ALEX samples include hexadecanoic acid, trimethyl ester, hexadecenoic acid, ethyl ester, 2(4H)-
Benzofuranone, 5,6,7,7a-tetrahydro-4,4,7a- trimethyl-, trans-9-Octadecenoic acid, trimethylsilyl ester
and 4-Isopropylphenol, trimethylsilyl ether. ALEX showed comparable anti-bacterial activity with zone
of inhibition of 6.0 - 15.7 mmfor gram positive bacteria and 2.0 - 13.0 mm for gram negative bacteria
against control which is Dimethyl sulfoxide (DMSO). Penicillin was used as positive controlexhibited
strong zone of inhibition of 21.0 – 28.3 mm. The antibacterial activity results indicate that
ALEXpossessed mild to stronginhibitory activities against the pathogenic bacteria due to presence of
various phytochemical constituents which can be used as source of drugs to fight infections caused by
susceptible bacteria. |
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