Anticancer potential and chemical profile of agarwood hydrosol
This is the first report discussing the chemical profile of agarwood hydrosol and its potential anticancer effects. Agarwood hydrosols, from two batches, obtained from Negeri Sembilan, Malaysia, were used in this study. Calu-3 cancer cells were used as a model cell line for lung cancer. The cells...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
UTM Press
2019
|
| Subjects: | |
| Online Access: | http://irep.iium.edu.my/77247/ http://irep.iium.edu.my/77247/ http://irep.iium.edu.my/77247/ http://irep.iium.edu.my/77247/1/77247_%20Anticancer%20potential%20and%20chemical.pdf |
| Summary: | This is the first report discussing the chemical profile of agarwood hydrosol and its potential
anticancer effects. Agarwood hydrosols, from two batches, obtained from Negeri Sembilan,
Malaysia, were used in this study. Calu-3 cancer cells were used as a model cell line for lung cancer.
The cells were cultured in Eagle’s minimum essential medium (EMEM) supplemented with 10% (v/v)
fetal bovine serum (FBS). The study was carried out in two phases, namely the anticancer study via
an attachment (AT) assay and cell viability (CV) assay, and then profiling the hydrosol from the two
batches via GCMS and hSPME-GCMS. Design Expert software’s two-factor face-centered central
composite design (FCCCD) was used to study the effects of agarwood hydrosol amount and time of
exposure on cell attachment (AT) and viability (CV). The findings suggest that agarwood hydrosols of
Aquilaria malaccensis possess both anti-attachment and cytotoxic effects on Calu-3 lung cancer
cells. A linear model was developed for anti-attachment effects and a quadratic model for the
cytotoxic effects with exposure duration being significant in both cases. From the profiling data, 1-
tricosene and 16-hentriacontanone were identified as potential contributors to the anti-attachment
and/or cytotoxic activity observed. In conclusion, agarwood hydrosol holds a potential to be further
investigated as a source of anti-cancer compounds. |
|---|