Single-tube, dual channel pentaplexing for the identification of Candida strains associated with human infection.

Invasive candidiasis is one of the most common nosocomial fungal infections worldwide. Delayed implementation of effective antifungal treatment caused by inefficient Candida diagnosis contributes to its notoriously high mortality rates. The availability of better Candida diagnostic tools would posit...

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Main Authors:	Jainlabdin, Mohd Hanif, Batra, Ambalika, Paredes, Edith Sánchez, Hernández Hernández, Francisca, Fu, Guoliang, Jorge, Tovar-Torres
Format:	Article
Language:	English English
Published:	Nat\ure Publishing Group 2019
Subjects:	QR Microbiology
Online Access:	http://irep.iium.edu.my/76777/ http://irep.iium.edu.my/76777/ http://irep.iium.edu.my/76777/ http://irep.iium.edu.my/76777/1/76777_Single-tube%2C%20dual%20channel_WOS.pdf http://irep.iium.edu.my/76777/2/76777_Single-tube%2C%20dual%20channel.pdf

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recordtype	eprints
spelling	iium-767772019-12-30T01:04:06Z http://irep.iium.edu.my/76777/ Single-tube, dual channel pentaplexing for the identification of Candida strains associated with human infection. Jainlabdin, Mohd Hanif Batra, Ambalika Paredes, Edith Sánchez Hernández Hernández, Francisca Fu, Guoliang Jorge, Tovar-Torres QR Microbiology Invasive candidiasis is one of the most common nosocomial fungal infections worldwide. Delayed implementation of effective antifungal treatment caused by inefficient Candida diagnosis contributes to its notoriously high mortality rates. The availability of better Candida diagnostic tools would positively impact patient outcomes. Here, we report on the development of a single-tube, dual channel pentaplex molecular diagnostic assay based on Multiplex Probe Amplification (MPA) technology. It allows simultaneous identification of C. auris, C. glabrata and C. krusei, at species-level as well as of six additional albicans and non-albicans pathogenic Candida at genus level. The assay overcomes the one-channel one-biomarker limitation of qPCR-based assays. Assay specificities are conferred by unique biomarker probe pairs with characteristic melting temperatures; post-amplification melting curve analysis allows simple identification of the infectious agent. Alerting for the presence of C. auris, the well-characterised multi-drug resistant outbreak strain, will facilitate informed therapy decisions and aid antifungal stewardship. The MPA-Candida assay can also be coupled to a pan-Fungal assay when differentiation between fungal and bacterial infections might be desirable. Its multiplexing capacity, detection range, specificity and sensitivity suggest the potential use of this novel MPA-Candida assay in clinical diagnosis and in the control and management of hospital outbreaks. Nat\ure Publishing Group 2019-10-11 Article PeerReviewed application/pdf en http://irep.iium.edu.my/76777/1/76777_Single-tube%2C%20dual%20channel_WOS.pdf application/pdf en http://irep.iium.edu.my/76777/2/76777_Single-tube%2C%20dual%20channel.pdf Jainlabdin, Mohd Hanif and Batra, Ambalika and Paredes, Edith Sánchez and Hernández Hernández, Francisca and Fu, Guoliang and Jorge, Tovar-Torres (2019) Single-tube, dual channel pentaplexing for the identification of Candida strains associated with human infection. Scientific Reports, 9. pp. 1-14. E-ISSN 2045-2322 https://www.nature.com/articles/s41598-019-51198-6.pdf https://doi.org/10.1038/s41598-019-51198-6
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	QR Microbiology
spellingShingle	QR Microbiology Jainlabdin, Mohd Hanif Batra, Ambalika Paredes, Edith Sánchez Hernández Hernández, Francisca Fu, Guoliang Jorge, Tovar-Torres Single-tube, dual channel pentaplexing for the identification of Candida strains associated with human infection.
description	Invasive candidiasis is one of the most common nosocomial fungal infections worldwide. Delayed implementation of effective antifungal treatment caused by inefficient Candida diagnosis contributes to its notoriously high mortality rates. The availability of better Candida diagnostic tools would positively impact patient outcomes. Here, we report on the development of a single-tube, dual channel pentaplex molecular diagnostic assay based on Multiplex Probe Amplification (MPA) technology. It allows simultaneous identification of C. auris, C. glabrata and C. krusei, at species-level as well as of six additional albicans and non-albicans pathogenic Candida at genus level. The assay overcomes the one-channel one-biomarker limitation of qPCR-based assays. Assay specificities are conferred by unique biomarker probe pairs with characteristic melting temperatures; post-amplification melting curve analysis allows simple identification of the infectious agent. Alerting for the presence of C. auris, the well-characterised multi-drug resistant outbreak strain, will facilitate informed therapy decisions and aid antifungal stewardship. The MPA-Candida assay can also be coupled to a pan-Fungal assay when differentiation between fungal and bacterial infections might be desirable. Its multiplexing capacity, detection range, specificity and sensitivity suggest the potential use of this novel MPA-Candida assay in clinical diagnosis and in the control and management of hospital outbreaks.
format	Article
author	Jainlabdin, Mohd Hanif Batra, Ambalika Paredes, Edith Sánchez Hernández Hernández, Francisca Fu, Guoliang Jorge, Tovar-Torres
author_facet	Jainlabdin, Mohd Hanif Batra, Ambalika Paredes, Edith Sánchez Hernández Hernández, Francisca Fu, Guoliang Jorge, Tovar-Torres
author_sort	Jainlabdin, Mohd Hanif
title	Single-tube, dual channel pentaplexing for the identification of Candida strains associated with human infection.
title_short	Single-tube, dual channel pentaplexing for the identification of Candida strains associated with human infection.
title_full	Single-tube, dual channel pentaplexing for the identification of Candida strains associated with human infection.
title_fullStr	Single-tube, dual channel pentaplexing for the identification of Candida strains associated with human infection.
title_full_unstemmed	Single-tube, dual channel pentaplexing for the identification of Candida strains associated with human infection.
title_sort	single-tube, dual channel pentaplexing for the identification of candida strains associated with human infection.
publisher	Nat\ure Publishing Group
publishDate	2019
url	http://irep.iium.edu.my/76777/ http://irep.iium.edu.my/76777/ http://irep.iium.edu.my/76777/ http://irep.iium.edu.my/76777/1/76777_Single-tube%2C%20dual%20channel_WOS.pdf http://irep.iium.edu.my/76777/2/76777_Single-tube%2C%20dual%20channel.pdf
first_indexed	2023-09-18T21:48:26Z
last_indexed	2023-09-18T21:48:26Z
_version_	1777413606995918848

Single-tube, dual channel pentaplexing for the identification of Candida strains associated with human infection.

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