Cross-species amplification of microsatellite DNA markers and comparison on the sequence of microsatellite regions among Southeast Asian Catfish species

Thirty microsatellite markers developed for River catfish (Hemibagrus nemurus) were screened for cross amplification in six species of catfish. Out of 30 primers, only 3 (10) to 6 (20) produced successful amplifications in six catfish species. Six different loci...

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Main Authors:	Fitri, Muhammad, Siraj, Siti Shapor, Nakajima, Masamichi
Format:	Article
Language:	English
Published:	The Japanese Society of Fish Genetics and Breeding Science 2015
Subjects:	SH Aquaculture. Fisheries. Angling SH151 Aquaculture - Fish Culture
Online Access:	http://irep.iium.edu.my/76559/ http://irep.iium.edu.my/76559/ http://irep.iium.edu.my/76559/1/Cross-Species_Amplification_of_Microsatellite_DNA_.pdf

id	iium-76559
recordtype	eprints
spelling	iium-765592019-12-26T08:35:10Z http://irep.iium.edu.my/76559/ Cross-species amplification of microsatellite DNA markers and comparison on the sequence of microsatellite regions among Southeast Asian Catfish species Fitri, Muhammad Siraj, Siti Shapor Nakajima, Masamichi SH Aquaculture. Fisheries. Angling SH151 Aquaculture - Fish Culture Thirty microsatellite markers developed for River catfish (Hemibagrus nemurus) were screened for cross amplification in six species of catfish. Out of 30 primers, only 3 (10) to 6 (20) produced successful amplifications in six catfish species. Six different loci could be amplified in Pangasius pangasius and Clarias batrachus, 5 loci in Clarias gariepinus, 4 loci in Pseudomystus siamensis and 3 loci in Clarias macrocephalus and Pangasius nasutus. The low rate of amplification in these 6 closely related species proved the primers are locus-specific. Sequence analysis of locus MnRm7-1 showed differences in the flanking region and repeat motif in certain species, suggesting the existence of indel mutations in catfish species in that particular region. The examined DNA sequence revealed that the repeat motif is conser ved within Pangasiid catfishes and not conser ved within Clariid catfishes The Japanese Society of Fish Genetics and Breeding Science 2015 Article PeerReviewed application/pdf en cc_public_domain http://irep.iium.edu.my/76559/1/Cross-Species_Amplification_of_Microsatellite_DNA_.pdf Fitri, Muhammad and Siraj, Siti Shapor and Nakajima, Masamichi (2015) Cross-species amplification of microsatellite DNA markers and comparison on the sequence of microsatellite regions among Southeast Asian Catfish species. Fish Genetics and Breeding Science, 43 (2). pp. 49-57. ISSN 1343-7917 http://www.suisan-ikushu.jp/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	SH Aquaculture. Fisheries. Angling SH151 Aquaculture - Fish Culture
spellingShingle	SH Aquaculture. Fisheries. Angling SH151 Aquaculture - Fish Culture Fitri, Muhammad Siraj, Siti Shapor Nakajima, Masamichi Cross-species amplification of microsatellite DNA markers and comparison on the sequence of microsatellite regions among Southeast Asian Catfish species
description	Thirty microsatellite markers developed for River catfish (Hemibagrus nemurus) were screened for cross amplification in six species of catfish. Out of 30 primers, only 3 (10) to 6 (20) produced successful amplifications in six catfish species. Six different loci could be amplified in Pangasius pangasius and Clarias batrachus, 5 loci in Clarias gariepinus, 4 loci in Pseudomystus siamensis and 3 loci in Clarias macrocephalus and Pangasius nasutus. The low rate of amplification in these 6 closely related species proved the primers are locus-specific. Sequence analysis of locus MnRm7-1 showed differences in the flanking region and repeat motif in certain species, suggesting the existence of indel mutations in catfish species in that particular region. The examined DNA sequence revealed that the repeat motif is conser ved within Pangasiid catfishes and not conser ved within Clariid catfishes
format	Article
author	Fitri, Muhammad Siraj, Siti Shapor Nakajima, Masamichi
author_facet	Fitri, Muhammad Siraj, Siti Shapor Nakajima, Masamichi
author_sort	Fitri, Muhammad
title	Cross-species amplification of microsatellite DNA markers and comparison on the sequence of microsatellite regions among Southeast Asian Catfish species
title_short	Cross-species amplification of microsatellite DNA markers and comparison on the sequence of microsatellite regions among Southeast Asian Catfish species
title_full	Cross-species amplification of microsatellite DNA markers and comparison on the sequence of microsatellite regions among Southeast Asian Catfish species
title_fullStr	Cross-species amplification of microsatellite DNA markers and comparison on the sequence of microsatellite regions among Southeast Asian Catfish species
title_full_unstemmed	Cross-species amplification of microsatellite DNA markers and comparison on the sequence of microsatellite regions among Southeast Asian Catfish species
title_sort	cross-species amplification of microsatellite dna markers and comparison on the sequence of microsatellite regions among southeast asian catfish species
publisher	The Japanese Society of Fish Genetics and Breeding Science
publishDate	2015
url	http://irep.iium.edu.my/76559/ http://irep.iium.edu.my/76559/ http://irep.iium.edu.my/76559/1/Cross-Species_Amplification_of_Microsatellite_DNA_.pdf
first_indexed	2023-09-18T21:48:08Z
last_indexed	2023-09-18T21:48:08Z
_version_	1777413588038713344

Cross-species amplification of microsatellite DNA markers and comparison on the sequence of microsatellite regions among Southeast Asian Catfish species

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