Structural integrity of aortic scaffolds decellularized by sonication decellularization system

Sonication decellularization technique has shown effectiveness to remove all the cellular components by the disruption of the cell membranes and removal of the cell debris to prepare the bioscaffolds. However, it is important to confirm whether this technique does not have a detrimental effect o...

Full description

Bibliographic Details
Main Authors:	, Aqilah Hazwani, Sha’ban, Munirah, Azhim, Azran
Format:	Conference or Workshop Item
Language:	English English
Published:	Institute of Electrical and Electronics Engineers Inc. 2019
Subjects:	Q Science (General) QR Microbiology R Medicine (General)
Online Access:	http://irep.iium.edu.my/76211/ http://irep.iium.edu.my/76211/ http://irep.iium.edu.my/76211/ http://irep.iium.edu.my/76211/1/76211_Structural%20Integrity%20of%20Aortic_scopus.pdf http://irep.iium.edu.my/76211/2/76211_Structural%20Integrity%20of%20Aortic_complete.pdf

id	iium-76211
recordtype	eprints
spelling	iium-762112019-11-13T13:42:37Z http://irep.iium.edu.my/76211/ Structural integrity of aortic scaffolds decellularized by sonication decellularization system , Aqilah Hazwani Sha’ban, Munirah Azhim, Azran Q Science (General) QR Microbiology R Medicine (General) Sonication decellularization technique has shown effectiveness to remove all the cellular components by the disruption of the cell membranes and removal of the cell debris to prepare the bioscaffolds. However, it is important to confirm whether this technique does not have a detrimental effect on elastin and collagen in bioscaffolds. The objectives of this study are to evaluate the structural integrity of bioscaffolds using histological staining and quantitatively collagen and elastin measurement. Aortic tissues were sonicated in 0.1% SDS for 10 hours at the frequency of 170 kHz with the power output of 15W and washed in Phosphate Buffer Solution (PBS) for 5 days. Then the sonicated aortic tissues were evaluated by Hematoxylin & Eosin (H&E) staining for cell removal analysis, Verhoeff-van Gieson (VVG) staining for visualizing elastin and Picrosirius Red (PSR) staining for visualizing collagen. The collagen and elastic fibres were semi-quantified by ImageJ software. The results showed that sonication decellularization system can remove all the cellular components while maintaining the structural integrity of elastin and collagen on bioscaffolds. This study indicates that sonication decellularization system could remove all cellular components and maintain the structure of the extracellular matrix. Institute of Electrical and Electronics Engineers Inc. 2019-01 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/76211/1/76211_Structural%20Integrity%20of%20Aortic_scopus.pdf application/pdf en http://irep.iium.edu.my/76211/2/76211_Structural%20Integrity%20of%20Aortic_complete.pdf , Aqilah Hazwani and Sha’ban, Munirah and Azhim, Azran (2019) Structural integrity of aortic scaffolds decellularized by sonication decellularization system. In: "2018 IEEE EMBS Conference on Biomedical Engineering and Sciences, IECBES 2018", 3 - 6 December 2018, Borneo Convention Centre KuchingDemak-Isthmus Bridge, Sejingkat Kuching, Sarawak. https://ieeexplore-ieee-org.ezproxy.um.edu.my/stamp/stamp.jsp?tp=&arnumber=8626723 10.1109/IECBES.2018.08626723
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	Q Science (General) QR Microbiology R Medicine (General)
spellingShingle	Q Science (General) QR Microbiology R Medicine (General) , Aqilah Hazwani Sha’ban, Munirah Azhim, Azran Structural integrity of aortic scaffolds decellularized by sonication decellularization system
description	Sonication decellularization technique has shown effectiveness to remove all the cellular components by the disruption of the cell membranes and removal of the cell debris to prepare the bioscaffolds. However, it is important to confirm whether this technique does not have a detrimental effect on elastin and collagen in bioscaffolds. The objectives of this study are to evaluate the structural integrity of bioscaffolds using histological staining and quantitatively collagen and elastin measurement. Aortic tissues were sonicated in 0.1% SDS for 10 hours at the frequency of 170 kHz with the power output of 15W and washed in Phosphate Buffer Solution (PBS) for 5 days. Then the sonicated aortic tissues were evaluated by Hematoxylin & Eosin (H&E) staining for cell removal analysis, Verhoeff-van Gieson (VVG) staining for visualizing elastin and Picrosirius Red (PSR) staining for visualizing collagen. The collagen and elastic fibres were semi-quantified by ImageJ software. The results showed that sonication decellularization system can remove all the cellular components while maintaining the structural integrity of elastin and collagen on bioscaffolds. This study indicates that sonication decellularization system could remove all cellular components and maintain the structure of the extracellular matrix.
format	Conference or Workshop Item
author	, Aqilah Hazwani Sha’ban, Munirah Azhim, Azran
author_facet	, Aqilah Hazwani Sha’ban, Munirah Azhim, Azran
author_sort	, Aqilah Hazwani
title	Structural integrity of aortic scaffolds decellularized by sonication decellularization system
title_short	Structural integrity of aortic scaffolds decellularized by sonication decellularization system
title_full	Structural integrity of aortic scaffolds decellularized by sonication decellularization system
title_fullStr	Structural integrity of aortic scaffolds decellularized by sonication decellularization system
title_full_unstemmed	Structural integrity of aortic scaffolds decellularized by sonication decellularization system
title_sort	structural integrity of aortic scaffolds decellularized by sonication decellularization system
publisher	Institute of Electrical and Electronics Engineers Inc.
publishDate	2019
url	http://irep.iium.edu.my/76211/ http://irep.iium.edu.my/76211/ http://irep.iium.edu.my/76211/ http://irep.iium.edu.my/76211/1/76211_Structural%20Integrity%20of%20Aortic_scopus.pdf http://irep.iium.edu.my/76211/2/76211_Structural%20Integrity%20of%20Aortic_complete.pdf
first_indexed	2023-09-18T21:47:43Z
last_indexed	2023-09-18T21:47:43Z
_version_	1777413562140983296

Structural integrity of aortic scaffolds decellularized by sonication decellularization system

Similar Items