Structural integrity of aortic scaffolds decellularized by sonication decellularization system
Sonication decellularization technique has shown effectiveness to remove all the cellular components by the disruption of the cell membranes and removal of the cell debris to prepare the bioscaffolds. However, it is important to confirm whether this technique does not have a detrimental effect o...
| Main Authors: | , , |
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| Format: | Conference or Workshop Item |
| Language: | English English |
| Published: |
Institute of Electrical and Electronics Engineers Inc.
2019
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/76211/ http://irep.iium.edu.my/76211/ http://irep.iium.edu.my/76211/ http://irep.iium.edu.my/76211/1/76211_Structural%20Integrity%20of%20Aortic_scopus.pdf http://irep.iium.edu.my/76211/2/76211_Structural%20Integrity%20of%20Aortic_complete.pdf |
| Summary: | Sonication decellularization technique has shown
effectiveness to remove all the cellular components by the
disruption of the cell membranes and removal of the cell debris
to prepare the bioscaffolds. However, it is important to confirm
whether this technique does not have a detrimental effect on
elastin and collagen in bioscaffolds. The objectives of this study
are to evaluate the structural integrity of bioscaffolds using
histological staining and quantitatively collagen and elastin
measurement. Aortic tissues were sonicated in 0.1% SDS for 10
hours at the frequency of 170 kHz with the power output of
15W and washed in Phosphate Buffer Solution (PBS) for 5
days. Then the sonicated aortic tissues were evaluated by
Hematoxylin & Eosin (H&E) staining for cell removal analysis,
Verhoeff-van Gieson (VVG) staining for visualizing elastin and
Picrosirius Red (PSR) staining for visualizing collagen. The
collagen and elastic fibres were semi-quantified by ImageJ
software. The results showed that sonication decellularization
system can remove all the cellular components while
maintaining the structural integrity of elastin and collagen on
bioscaffolds. This study indicates that sonication
decellularization system could remove all cellular components
and maintain the structure of the extracellular matrix. |
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