Phenotypic and genotypic characterization, and detection of PVL encoding gene in methicillin resistant staphylococcus aureus strains isolated from patients admitted to a tertiary Hospital in Kuantan, Malaysia

Introduction: Methicillin-resistant Staphylococcus aureus is globally a major public health threat. Resistance to methicillin originates from a modified protein (PBP2a) encoded by the mecA gene. The PVL gene as an important virulence factor increases the pathogenicity of MRSA. Epidemiology and cha...

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Main Authors:	Bariman, Mohammad Hanif, Mustafa Mahmoud, Mohammed Imad Al-Deen, Hamzah, Hairul Aini
Format:	Article
Language:	English English English
Published:	Kulliyyah of Medicine, International Islamic University Malaysia 2019
Subjects:	R Medicine (General) RA966 Medical Centers. Hospitals. Dispensaries. Clinics
Online Access:	http://irep.iium.edu.my/76177/ http://irep.iium.edu.my/76177/ http://irep.iium.edu.my/76177/13/76177_Phenotypic%20and%20genotypic%20characterization.pdf http://irep.iium.edu.my/76177/2/76177_Phenotypic%20and%20genotypic%20characterization%2C%20and%20detection%20of%20PVL%20encoding%20gene%20in%20methicillin%20resistant%20staphylococcus%20aureus_SCOPUS.pdf http://irep.iium.edu.my/76177/14/76177_Phenotypic%20and%20genotypic%20characterization_WOS.pdf

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spelling	iium-761772019-12-16T08:52:00Z http://irep.iium.edu.my/76177/ Phenotypic and genotypic characterization, and detection of PVL encoding gene in methicillin resistant staphylococcus aureus strains isolated from patients admitted to a tertiary Hospital in Kuantan, Malaysia Bariman, Mohammad Hanif Mustafa Mahmoud, Mohammed Imad Al-Deen Hamzah, Hairul Aini R Medicine (General) RA966 Medical Centers. Hospitals. Dispensaries. Clinics Introduction: Methicillin-resistant Staphylococcus aureus is globally a major public health threat. Resistance to methicillin originates from a modified protein (PBP2a) encoded by the mecA gene. The PVL gene as an important virulence factor increases the pathogenicity of MRSA. Epidemiology and characteristics of MRSA differ in different geographical regions. This study was conducted to characterize and determine the antibiotic susceptibility profile of MRSA strains isolated from patients in Hospital Tengku Ampuan Afzan, Pahang, Malaysia and to detect the presence of the mecA and PVL genes in the isolates. Materials and methods: In this study a total of 36 isolates of MRSA have been collected during a period of three months (1stFebruary –30thApril 2018). The susceptibility pattern of the isolates to ten different commonly used antibiotics were determined and the target genes were addressed by real-time PCR experiment. Results: Based on the identifying criteria, 44.4% of the isolates were CA-MRSA, and 55.5% were HA-MRSA. Resistance to oxacillin, cefoxitin and penicillin was 100%, gentamicin 88.8%, erythromycin 33.3%, tetracycline 77.7%, trimethoprim-sulfamethoxazole 61.1%, clindamycin 13.8%, chloramphenicol 11.1%, but no resistant strain of vancomycin was detected. Most of the isolates were resistant to more than three groups of antibiotics. Realtime PCR revealed that all the isolates were mecA positive and 4 isolates were PVL-positive. PVL-positive strains were CA-MRSA and susceptible to clindamycin. Conclusion: The study confirms multi-drug resistant MRSA in the study area, and shows that resistance to methicillin is mecA mediated. PVL carrier strains were present and related to CA-MRSA strains of the isolates. Kulliyyah of Medicine, International Islamic University Malaysia 2019-08-01 Article PeerReviewed application/pdf en http://irep.iium.edu.my/76177/13/76177_Phenotypic%20and%20genotypic%20characterization.pdf application/pdf en http://irep.iium.edu.my/76177/2/76177_Phenotypic%20and%20genotypic%20characterization%2C%20and%20detection%20of%20PVL%20encoding%20gene%20in%20methicillin%20resistant%20staphylococcus%20aureus_SCOPUS.pdf application/pdf en http://irep.iium.edu.my/76177/14/76177_Phenotypic%20and%20genotypic%20characterization_WOS.pdf Bariman, Mohammad Hanif and Mustafa Mahmoud, Mohammed Imad Al-Deen and Hamzah, Hairul Aini (2019) Phenotypic and genotypic characterization, and detection of PVL encoding gene in methicillin resistant staphylococcus aureus strains isolated from patients admitted to a tertiary Hospital in Kuantan, Malaysia. International Medical Journal Malaysia (IMJM), 18 (2). pp. 87-94. ISSN 1823-4631 https://journals.iium.edu.my/kom/index.php/imjm/article/view/92/17
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English
topic	R Medicine (General) RA966 Medical Centers. Hospitals. Dispensaries. Clinics
spellingShingle	R Medicine (General) RA966 Medical Centers. Hospitals. Dispensaries. Clinics Bariman, Mohammad Hanif Mustafa Mahmoud, Mohammed Imad Al-Deen Hamzah, Hairul Aini Phenotypic and genotypic characterization, and detection of PVL encoding gene in methicillin resistant staphylococcus aureus strains isolated from patients admitted to a tertiary Hospital in Kuantan, Malaysia
description	Introduction: Methicillin-resistant Staphylococcus aureus is globally a major public health threat. Resistance to methicillin originates from a modified protein (PBP2a) encoded by the mecA gene. The PVL gene as an important virulence factor increases the pathogenicity of MRSA. Epidemiology and characteristics of MRSA differ in different geographical regions. This study was conducted to characterize and determine the antibiotic susceptibility profile of MRSA strains isolated from patients in Hospital Tengku Ampuan Afzan, Pahang, Malaysia and to detect the presence of the mecA and PVL genes in the isolates. Materials and methods: In this study a total of 36 isolates of MRSA have been collected during a period of three months (1stFebruary –30thApril 2018). The susceptibility pattern of the isolates to ten different commonly used antibiotics were determined and the target genes were addressed by real-time PCR experiment. Results: Based on the identifying criteria, 44.4% of the isolates were CA-MRSA, and 55.5% were HA-MRSA. Resistance to oxacillin, cefoxitin and penicillin was 100%, gentamicin 88.8%, erythromycin 33.3%, tetracycline 77.7%, trimethoprim-sulfamethoxazole 61.1%, clindamycin 13.8%, chloramphenicol 11.1%, but no resistant strain of vancomycin was detected. Most of the isolates were resistant to more than three groups of antibiotics. Realtime PCR revealed that all the isolates were mecA positive and 4 isolates were PVL-positive. PVL-positive strains were CA-MRSA and susceptible to clindamycin. Conclusion: The study confirms multi-drug resistant MRSA in the study area, and shows that resistance to methicillin is mecA mediated. PVL carrier strains were present and related to CA-MRSA strains of the isolates.
format	Article
author	Bariman, Mohammad Hanif Mustafa Mahmoud, Mohammed Imad Al-Deen Hamzah, Hairul Aini
author_facet	Bariman, Mohammad Hanif Mustafa Mahmoud, Mohammed Imad Al-Deen Hamzah, Hairul Aini
author_sort	Bariman, Mohammad Hanif
title	Phenotypic and genotypic characterization, and detection of PVL encoding gene in methicillin resistant staphylococcus aureus strains isolated from patients admitted to a tertiary Hospital in Kuantan, Malaysia
title_short	Phenotypic and genotypic characterization, and detection of PVL encoding gene in methicillin resistant staphylococcus aureus strains isolated from patients admitted to a tertiary Hospital in Kuantan, Malaysia
title_full	Phenotypic and genotypic characterization, and detection of PVL encoding gene in methicillin resistant staphylococcus aureus strains isolated from patients admitted to a tertiary Hospital in Kuantan, Malaysia
title_fullStr	Phenotypic and genotypic characterization, and detection of PVL encoding gene in methicillin resistant staphylococcus aureus strains isolated from patients admitted to a tertiary Hospital in Kuantan, Malaysia
title_full_unstemmed	Phenotypic and genotypic characterization, and detection of PVL encoding gene in methicillin resistant staphylococcus aureus strains isolated from patients admitted to a tertiary Hospital in Kuantan, Malaysia
title_sort	phenotypic and genotypic characterization, and detection of pvl encoding gene in methicillin resistant staphylococcus aureus strains isolated from patients admitted to a tertiary hospital in kuantan, malaysia
publisher	Kulliyyah of Medicine, International Islamic University Malaysia
publishDate	2019
url	http://irep.iium.edu.my/76177/ http://irep.iium.edu.my/76177/ http://irep.iium.edu.my/76177/13/76177_Phenotypic%20and%20genotypic%20characterization.pdf http://irep.iium.edu.my/76177/2/76177_Phenotypic%20and%20genotypic%20characterization%2C%20and%20detection%20of%20PVL%20encoding%20gene%20in%20methicillin%20resistant%20staphylococcus%20aureus_SCOPUS.pdf http://irep.iium.edu.my/76177/14/76177_Phenotypic%20and%20genotypic%20characterization_WOS.pdf
first_indexed	2023-09-18T21:47:41Z
last_indexed	2023-09-18T21:47:41Z
_version_	1777413559544709120

Phenotypic and genotypic characterization, and detection of PVL encoding gene in methicillin resistant staphylococcus aureus strains isolated from patients admitted to a tertiary Hospital in Kuantan, Malaysia

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