Antioxidant activity and in vitro cytotoxicity study of the phenolic compounds from piper sarmentosum.

Cancer is by far one of the most worrying health issues that continues to be the major killing diseases worldwide, accounting for more than 6 million deaths. In the confrontation of advances in modern medicine such as surgery, chemotherapy, radiotherapy and hormone therapy, cancer disease persists a...

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Main Authors:	Ibrahim, Maizatul Akma, Ghazali, Nor Farahiyah, Mustafa, Farah Farisha, Abdul, Nurul Huda, Tengku Muhammad2, Tengku Sifzizul
Format:	Conference or Workshop Item
Language:	English
Published:	2019
Subjects:	Q Science (General) QK Botany R Medicine (General) RS Pharmacy and materia medica RV Botanic, Thomsonian, and eclectic medicine
Online Access:	http://irep.iium.edu.my/76130/ http://irep.iium.edu.my/76130/1/76130_ANTIOXIDANT%20ACTIVITY%20AND%20IN%20VITRO%20CYTOTOXICITY_complete.pdf

id	iium-76130
recordtype	eprints
spelling	iium-761302019-12-10T09:10:43Z http://irep.iium.edu.my/76130/ Antioxidant activity and in vitro cytotoxicity study of the phenolic compounds from piper sarmentosum. Ibrahim, Maizatul Akma Ghazali, Nor Farahiyah Mustafa, Farah Farisha Abdul, Nurul Huda Tengku Muhammad2, Tengku Sifzizul Q Science (General) QK Botany R Medicine (General) RS Pharmacy and materia medica RV Botanic, Thomsonian, and eclectic medicine Cancer is by far one of the most worrying health issues that continues to be the major killing diseases worldwide, accounting for more than 6 million deaths. In the confrontation of advances in modern medicine such as surgery, chemotherapy, radiotherapy and hormone therapy, cancer disease persists a worldwide problem. Thus the search for new potential anticancer agents avertable to human malignancies is highly demanded. Since the past few years, the search for new alternatives has been concentrated in finding medicinal herbs from local natural resources. Approximately, 60% of currently applied cancer drugs are derived from natural sources, due to its fewer side effects. Piper sarmentosum Roxb. which is locally known as ‘kaduk’, is one of natural medicinal plant that had been used traditionally to treat headache, arthritis, menstrual pain, cough and eczema. It is proven to have various biological properties such as anti-inflammatory, antioxidant, antimalarial, antiplasmodial, anti-diabetic, antifungal and anticarcinogenic. The aims of this study were to identify the phenolic compounds of P. sarmentosum methanolic extracts by measuring total phenolic content, and HPLC analysis. The antioxidant activity was evaluated by DPPH scavenging assay. MTS assay was also conducted to study the effect of the extracts on human breast cancer cells, MCF-7. The HPLC analysis showed the presence of quercetin, naringin, gallic acid and tannic acid in P. sarmentosum. P. sarmentosum exhibited antioxidant property at 96.98 ± 2.29 μg/mL by DPPH scavenging activity with its high phenolic content at 89.22 mg GAE/ g dry extract. The cytotoxicity screening of P. sarmentosum extract using MTS assay provided IC50 of 24.63 ± 0.23 μg/mL on MCF-7 cells. In conclusion, the phenolic compounds obtained from P. sarmentosum possess potent antioxidant and anticancer activity against the tested breast cancer cells. Further study might focus on the compounds action mechanisms toward the tumour cells. 2019-11 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/76130/1/76130_ANTIOXIDANT%20ACTIVITY%20AND%20IN%20VITRO%20CYTOTOXICITY_complete.pdf Ibrahim, Maizatul Akma and Ghazali, Nor Farahiyah and Mustafa, Farah Farisha and Abdul, Nurul Huda and Tengku Muhammad2, Tengku Sifzizul (2019) Antioxidant activity and in vitro cytotoxicity study of the phenolic compounds from piper sarmentosum. In: International Environment And Health Conference 2019, Kota Bharu, Kelantan. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	Q Science (General) QK Botany R Medicine (General) RS Pharmacy and materia medica RV Botanic, Thomsonian, and eclectic medicine
spellingShingle	Q Science (General) QK Botany R Medicine (General) RS Pharmacy and materia medica RV Botanic, Thomsonian, and eclectic medicine Ibrahim, Maizatul Akma Ghazali, Nor Farahiyah Mustafa, Farah Farisha Abdul, Nurul Huda Tengku Muhammad2, Tengku Sifzizul Antioxidant activity and in vitro cytotoxicity study of the phenolic compounds from piper sarmentosum.
description	Cancer is by far one of the most worrying health issues that continues to be the major killing diseases worldwide, accounting for more than 6 million deaths. In the confrontation of advances in modern medicine such as surgery, chemotherapy, radiotherapy and hormone therapy, cancer disease persists a worldwide problem. Thus the search for new potential anticancer agents avertable to human malignancies is highly demanded. Since the past few years, the search for new alternatives has been concentrated in finding medicinal herbs from local natural resources. Approximately, 60% of currently applied cancer drugs are derived from natural sources, due to its fewer side effects. Piper sarmentosum Roxb. which is locally known as ‘kaduk’, is one of natural medicinal plant that had been used traditionally to treat headache, arthritis, menstrual pain, cough and eczema. It is proven to have various biological properties such as anti-inflammatory, antioxidant, antimalarial, antiplasmodial, anti-diabetic, antifungal and anticarcinogenic. The aims of this study were to identify the phenolic compounds of P. sarmentosum methanolic extracts by measuring total phenolic content, and HPLC analysis. The antioxidant activity was evaluated by DPPH scavenging assay. MTS assay was also conducted to study the effect of the extracts on human breast cancer cells, MCF-7. The HPLC analysis showed the presence of quercetin, naringin, gallic acid and tannic acid in P. sarmentosum. P. sarmentosum exhibited antioxidant property at 96.98 ± 2.29 μg/mL by DPPH scavenging activity with its high phenolic content at 89.22 mg GAE/ g dry extract. The cytotoxicity screening of P. sarmentosum extract using MTS assay provided IC50 of 24.63 ± 0.23 μg/mL on MCF-7 cells. In conclusion, the phenolic compounds obtained from P. sarmentosum possess potent antioxidant and anticancer activity against the tested breast cancer cells. Further study might focus on the compounds action mechanisms toward the tumour cells.
format	Conference or Workshop Item
author	Ibrahim, Maizatul Akma Ghazali, Nor Farahiyah Mustafa, Farah Farisha Abdul, Nurul Huda Tengku Muhammad2, Tengku Sifzizul
author_facet	Ibrahim, Maizatul Akma Ghazali, Nor Farahiyah Mustafa, Farah Farisha Abdul, Nurul Huda Tengku Muhammad2, Tengku Sifzizul
author_sort	Ibrahim, Maizatul Akma
title	Antioxidant activity and in vitro cytotoxicity study of the phenolic compounds from piper sarmentosum.
title_short	Antioxidant activity and in vitro cytotoxicity study of the phenolic compounds from piper sarmentosum.
title_full	Antioxidant activity and in vitro cytotoxicity study of the phenolic compounds from piper sarmentosum.
title_fullStr	Antioxidant activity and in vitro cytotoxicity study of the phenolic compounds from piper sarmentosum.
title_full_unstemmed	Antioxidant activity and in vitro cytotoxicity study of the phenolic compounds from piper sarmentosum.
title_sort	antioxidant activity and in vitro cytotoxicity study of the phenolic compounds from piper sarmentosum.
publishDate	2019
url	http://irep.iium.edu.my/76130/ http://irep.iium.edu.my/76130/1/76130_ANTIOXIDANT%20ACTIVITY%20AND%20IN%20VITRO%20CYTOTOXICITY_complete.pdf
first_indexed	2023-09-18T21:47:37Z
last_indexed	2023-09-18T21:47:37Z
_version_	1777413555575848960

Antioxidant activity and in vitro cytotoxicity study of the phenolic compounds from piper sarmentosum.

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