Comparative study of thaumatin protein from Thaumatococcus daniellii grown at different growth environments

Introduction: This study was carried out to investigate thaumatin profile in Thaumatococcus daniellii fruit cultivated in different growth condition. Methods: Three different samples used were T. daniellii that grown naturally, T. daniellii treated with low fertilizer and T. daniellii treated with h...

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Main Authors:	Md Zin, Noor Hasniza, Ahmad Radzi, Muhammad Ahsanuddin, Abdul Wahab, Widya, Mohd Khairlani, Nurul Hidayah
Format:	Article
Language:	English
Published:	Kulliyyah of Allied Health Sciences, International Islamic University Malaysia. 2019
Subjects:	QH301 Biology
Online Access:	http://irep.iium.edu.my/76122/ http://irep.iium.edu.my/76122/ http://irep.iium.edu.my/76122/1/IJAHS%201_185-Article%20Text-1130-5-10-20190809.pdf

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spelling	iium-761222019-12-09T01:31:46Z http://irep.iium.edu.my/76122/ Comparative study of thaumatin protein from Thaumatococcus daniellii grown at different growth environments Md Zin, Noor Hasniza Ahmad Radzi, Muhammad Ahsanuddin Abdul Wahab, Widya Mohd Khairlani, Nurul Hidayah QH301 Biology Introduction: This study was carried out to investigate thaumatin profile in Thaumatococcus daniellii fruit cultivated in different growth condition. Methods: Three different samples used were T. daniellii that grown naturally, T. daniellii treated with low fertilizer and T. daniellii treated with high fertilizer. The aril part of the fruit contains intense sweetener protein that can substitute sugar and other artificial sweetener was analaysed by Bradford assay and SDS-PAGE for determination of protein content and differences in protein expression repectively. Results: The total protein concentration of each sample calculated using Bradford assay were 0.367 ± 0.007 μg/μl, 0.343 ± 0.014 μg/μl and 0.426 ± 0.027 μg/μl for T. danielli grown at natural condition (uncontrolled environment), T. daniellii treated with low fertilizer and T. daniellii treated with high fertilizer respectively. All T. daniellii samples showed the presence of thaumatin as shown by the SDS-PAGE image. Only a single band appeared on the SDS-PAGE at the molecular weight of 22 kDa which is the actual size for thaumatin. The band intensity of T. danielli treated with high fertilizer appeared to be highest compared to the other two samples showing high in protein expression. Conclusion: This finding will be useful for other researchers to find out more about this plant for thaumatin production with the best growth condition. Kulliyyah of Allied Health Sciences, International Islamic University Malaysia. 2019 Article PeerReviewed application/pdf en http://irep.iium.edu.my/76122/1/IJAHS%201_185-Article%20Text-1130-5-10-20190809.pdf Md Zin, Noor Hasniza and Ahmad Radzi, Muhammad Ahsanuddin and Abdul Wahab, Widya and Mohd Khairlani, Nurul Hidayah (2019) Comparative study of thaumatin protein from Thaumatococcus daniellii grown at different growth environments. International Journal of Allied Health Sciences, 3 (2). pp. 668-674. E-ISSN 2600-8491 https://journals.iium.edu.my/ijahs/index.php/IJAHS
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QH301 Biology
spellingShingle	QH301 Biology Md Zin, Noor Hasniza Ahmad Radzi, Muhammad Ahsanuddin Abdul Wahab, Widya Mohd Khairlani, Nurul Hidayah Comparative study of thaumatin protein from Thaumatococcus daniellii grown at different growth environments
description	Introduction: This study was carried out to investigate thaumatin profile in Thaumatococcus daniellii fruit cultivated in different growth condition. Methods: Three different samples used were T. daniellii that grown naturally, T. daniellii treated with low fertilizer and T. daniellii treated with high fertilizer. The aril part of the fruit contains intense sweetener protein that can substitute sugar and other artificial sweetener was analaysed by Bradford assay and SDS-PAGE for determination of protein content and differences in protein expression repectively. Results: The total protein concentration of each sample calculated using Bradford assay were 0.367 ± 0.007 μg/μl, 0.343 ± 0.014 μg/μl and 0.426 ± 0.027 μg/μl for T. danielli grown at natural condition (uncontrolled environment), T. daniellii treated with low fertilizer and T. daniellii treated with high fertilizer respectively. All T. daniellii samples showed the presence of thaumatin as shown by the SDS-PAGE image. Only a single band appeared on the SDS-PAGE at the molecular weight of 22 kDa which is the actual size for thaumatin. The band intensity of T. danielli treated with high fertilizer appeared to be highest compared to the other two samples showing high in protein expression. Conclusion: This finding will be useful for other researchers to find out more about this plant for thaumatin production with the best growth condition.
format	Article
author	Md Zin, Noor Hasniza Ahmad Radzi, Muhammad Ahsanuddin Abdul Wahab, Widya Mohd Khairlani, Nurul Hidayah
author_facet	Md Zin, Noor Hasniza Ahmad Radzi, Muhammad Ahsanuddin Abdul Wahab, Widya Mohd Khairlani, Nurul Hidayah
author_sort	Md Zin, Noor Hasniza
title	Comparative study of thaumatin protein from Thaumatococcus daniellii grown at different growth environments
title_short	Comparative study of thaumatin protein from Thaumatococcus daniellii grown at different growth environments
title_full	Comparative study of thaumatin protein from Thaumatococcus daniellii grown at different growth environments
title_fullStr	Comparative study of thaumatin protein from Thaumatococcus daniellii grown at different growth environments
title_full_unstemmed	Comparative study of thaumatin protein from Thaumatococcus daniellii grown at different growth environments
title_sort	comparative study of thaumatin protein from thaumatococcus daniellii grown at different growth environments
publisher	Kulliyyah of Allied Health Sciences, International Islamic University Malaysia.
publishDate	2019
url	http://irep.iium.edu.my/76122/ http://irep.iium.edu.my/76122/ http://irep.iium.edu.my/76122/1/IJAHS%201_185-Article%20Text-1130-5-10-20190809.pdf
first_indexed	2023-09-18T21:47:36Z
last_indexed	2023-09-18T21:47:36Z
_version_	1777413554836602880

Comparative study of thaumatin protein from Thaumatococcus daniellii grown at different growth environments

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