The use of active melioidosis detect tm in early diagnosis of melioidosis in Hospital Tengku Ampuan Afzan and Hospital Sultanah Nurzahirah

NTRODUCTION: Melioidosis is endemic in Malaysia and an important cause of sepsis. Current gold standard for diagnosis is by culture method,but its long procedure will delay the treatment leading to hospital-related mortality.Thus,a good rapid test is needed to reduce its mortality burden. Recently,...

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Main Authors:	Wan Ramli, Wan Nurliyana, How, Soon Hin, Dzawani, M, W.O, Wan Izzah Hafizah, A. Kashfi, A.R., N, Noorlina, Baharudin, Roesnita
Format:	Conference or Workshop Item
Language:	English English
Published:	2019
Subjects:	RC Internal medicine RC111 Infectious and Parasitic Diseases
Online Access:	http://irep.iium.edu.my/75767/ http://irep.iium.edu.my/75767/7/MRS%20poster.pdf http://irep.iium.edu.my/75767/19/MRS%202019%20Abstract%20Book.pdf

id	iium-75767
recordtype	eprints
spelling	iium-757672020-01-02T10:01:09Z http://irep.iium.edu.my/75767/ The use of active melioidosis detect tm in early diagnosis of melioidosis in Hospital Tengku Ampuan Afzan and Hospital Sultanah Nurzahirah Wan Ramli, Wan Nurliyana How, Soon Hin Dzawani, M W.O, Wan Izzah Hafizah A. Kashfi, A.R. N, Noorlina Baharudin, Roesnita RC Internal medicine RC111 Infectious and Parasitic Diseases NTRODUCTION: Melioidosis is endemic in Malaysia and an important cause of sepsis. Current gold standard for diagnosis is by culture method,but its long procedure will delay the treatment leading to hospital-related mortality.Thus,a good rapid test is needed to reduce its mortality burden. Recently, Active Melioidosis Detect (AMD)have been shown to be useful. OBJECTIVES: (1)To measure the sensitivity and specificity of AMD.(2)To study the sensitivity and specificity of early morning urine AMD compared to spot urine AMD. METHOD : A prospective cross-sectional study of clinically suspected melioidosis patients in HTAA and HSNZ from April until December 2018. Blood and urine samples were tested with AMD. Test results were analysed for sensitivity, specificity,positive predictive value and negative predictive value. RESULTS: A total of 89 patients were included in this study.The mean age is 52 years old, and 56.3% were male gender.64% of patients have diabetes mellitus.11 patients have positive blood culture for Bukholderia pseudomallei, 4 of them were tested positive for AMD.3 of them presented with septic shock (3.4%), however none died.The sensitivity of the AMD was 36.4% ([95% CI 12.4 to 68.4]) and the specificity was 66.7% ( [95% CI 46.0 to 82.8]) in all samples, with positive predictive value of 30.7% and negative predictive value of 72%.Blood samples have lower sensitivity of 9.1% ([95% CI 4.8 to 42.9]) with high specificity of 100% ([95% CI 84.5 to 100]). Urine spot samples have higher sensitivity compared to serum and morning urine, with 36.4% ([95%CI 12.4 to 68.4]) and specificity of 88.9% ([95% CI 69.7 to 97.1]). CONCLUSION: From this pilot study, this test requires further evaluation before incorporating as point of care assay. 2019-10-09 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/75767/7/MRS%20poster.pdf application/pdf en http://irep.iium.edu.my/75767/19/MRS%202019%20Abstract%20Book.pdf Wan Ramli, Wan Nurliyana and How, Soon Hin and Dzawani, M and W.O, Wan Izzah Hafizah and A. Kashfi, A.R. and N, Noorlina and Baharudin, Roesnita (2019) The use of active melioidosis detect tm in early diagnosis of melioidosis in Hospital Tengku Ampuan Afzan and Hospital Sultanah Nurzahirah. In: Medical Research Symposium 2019: Expanding Research Collaboration in Health, 9 October 2019, IIUM Medical Centre, Kuantan Pahang. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	RC Internal medicine RC111 Infectious and Parasitic Diseases
spellingShingle	RC Internal medicine RC111 Infectious and Parasitic Diseases Wan Ramli, Wan Nurliyana How, Soon Hin Dzawani, M W.O, Wan Izzah Hafizah A. Kashfi, A.R. N, Noorlina Baharudin, Roesnita The use of active melioidosis detect tm in early diagnosis of melioidosis in Hospital Tengku Ampuan Afzan and Hospital Sultanah Nurzahirah
description	NTRODUCTION: Melioidosis is endemic in Malaysia and an important cause of sepsis. Current gold standard for diagnosis is by culture method,but its long procedure will delay the treatment leading to hospital-related mortality.Thus,a good rapid test is needed to reduce its mortality burden. Recently, Active Melioidosis Detect (AMD)have been shown to be useful. OBJECTIVES: (1)To measure the sensitivity and specificity of AMD.(2)To study the sensitivity and specificity of early morning urine AMD compared to spot urine AMD. METHOD : A prospective cross-sectional study of clinically suspected melioidosis patients in HTAA and HSNZ from April until December 2018. Blood and urine samples were tested with AMD. Test results were analysed for sensitivity, specificity,positive predictive value and negative predictive value. RESULTS: A total of 89 patients were included in this study.The mean age is 52 years old, and 56.3% were male gender.64% of patients have diabetes mellitus.11 patients have positive blood culture for Bukholderia pseudomallei, 4 of them were tested positive for AMD.3 of them presented with septic shock (3.4%), however none died.The sensitivity of the AMD was 36.4% ([95% CI 12.4 to 68.4]) and the specificity was 66.7% ( [95% CI 46.0 to 82.8]) in all samples, with positive predictive value of 30.7% and negative predictive value of 72%.Blood samples have lower sensitivity of 9.1% ([95% CI 4.8 to 42.9]) with high specificity of 100% ([95% CI 84.5 to 100]). Urine spot samples have higher sensitivity compared to serum and morning urine, with 36.4% ([95%CI 12.4 to 68.4]) and specificity of 88.9% ([95% CI 69.7 to 97.1]). CONCLUSION: From this pilot study, this test requires further evaluation before incorporating as point of care assay.
format	Conference or Workshop Item
author	Wan Ramli, Wan Nurliyana How, Soon Hin Dzawani, M W.O, Wan Izzah Hafizah A. Kashfi, A.R. N, Noorlina Baharudin, Roesnita
author_facet	Wan Ramli, Wan Nurliyana How, Soon Hin Dzawani, M W.O, Wan Izzah Hafizah A. Kashfi, A.R. N, Noorlina Baharudin, Roesnita
author_sort	Wan Ramli, Wan Nurliyana
title	The use of active melioidosis detect tm in early diagnosis of melioidosis in Hospital Tengku Ampuan Afzan and Hospital Sultanah Nurzahirah
title_short	The use of active melioidosis detect tm in early diagnosis of melioidosis in Hospital Tengku Ampuan Afzan and Hospital Sultanah Nurzahirah
title_full	The use of active melioidosis detect tm in early diagnosis of melioidosis in Hospital Tengku Ampuan Afzan and Hospital Sultanah Nurzahirah
title_fullStr	The use of active melioidosis detect tm in early diagnosis of melioidosis in Hospital Tengku Ampuan Afzan and Hospital Sultanah Nurzahirah
title_full_unstemmed	The use of active melioidosis detect tm in early diagnosis of melioidosis in Hospital Tengku Ampuan Afzan and Hospital Sultanah Nurzahirah
title_sort	use of active melioidosis detect tm in early diagnosis of melioidosis in hospital tengku ampuan afzan and hospital sultanah nurzahirah
publishDate	2019
url	http://irep.iium.edu.my/75767/ http://irep.iium.edu.my/75767/7/MRS%20poster.pdf http://irep.iium.edu.my/75767/19/MRS%202019%20Abstract%20Book.pdf
first_indexed	2023-09-18T21:47:11Z
last_indexed	2023-09-18T21:47:11Z
_version_	1777413528026611712

The use of active melioidosis detect tm in early diagnosis of melioidosis in Hospital Tengku Ampuan Afzan and Hospital Sultanah Nurzahirah

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