Effects of flaxseed (Linum usitatissimum) extract on osteoblast differentiation potential of stem cells derived from human exfoliated deciduous teeth (SHED)

Introduction: Flaxseed extract promotes bone health and possibly function in inducing bone regeneration process. SHED have been considered as an alternative source of MSC in tissue engineering because of their potential to differentiate into multiple cell-lineages including osteoblasts. Although fla...

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Main Authors:	Nordin, Nur Sazwi, Mokhtar@Makhtar, Khairani Idah, Mustafa Al-Ahmad, Basma Ezzat, Lestari, Widya, Arief Ichwan, Solachuddin Jauhari, Darnis, Deny Susanti, Thirumulu Ponnuraj, Kannan, Ahmad, Azlina
Format:	Conference or Workshop Item
Language:	English English English
Published:	2019
Subjects:	RK Dentistry
Online Access:	http://irep.iium.edu.my/75491/ http://irep.iium.edu.my/75491/1/75491_ICPRP%202019%20%28Cover%29.pdf http://irep.iium.edu.my/75491/2/75491_ICPRP%202019%20%28Program%20Book%29.pdf http://irep.iium.edu.my/75491/3/75491_ICPRP%202019%20%28Poster%29.pdf

id	iium-75491
recordtype	eprints
spelling	iium-754912019-11-18T04:50:07Z http://irep.iium.edu.my/75491/ Effects of flaxseed (Linum usitatissimum) extract on osteoblast differentiation potential of stem cells derived from human exfoliated deciduous teeth (SHED) Nordin, Nur Sazwi Mokhtar@Makhtar, Khairani Idah Mustafa Al-Ahmad, Basma Ezzat Lestari, Widya Arief Ichwan, Solachuddin Jauhari Darnis, Deny Susanti Thirumulu Ponnuraj, Kannan Ahmad, Azlina RK Dentistry Introduction: Flaxseed extract promotes bone health and possibly function in inducing bone regeneration process. SHED have been considered as an alternative source of MSC in tissue engineering because of their potential to differentiate into multiple cell-lineages including osteoblasts. Although flaxseed extract has been shown to affect bone formation process, its effect in inducing stem cell into osteoblast lineage has not been reported. Objective: To determine the effects of flaxseed extract on osteoblast differentiation potential of SHED. Materials and methods: SHED cultured in osteoblast induction media (OIM) was treated with flaxseed extract at 4mg/ml. The RNA was collected and extracted with Total RNA Mini Kit (Geneaid) from cells cultured at day 1, 3, 7, 14 and 21 and subjected to reverse-transcriptase PCR for osteoblast markers (OSX, OCN, DMP1). Alkaline phosphatase activity was determined by ALP assay and Alizarin Red S staining was performed to evaluate calcium deposits in SHED. Results: All osteoblast markers were expressed in all samples analysed. Interestingly, the expression of OSX appeared to be reduced in SHED treated with flaxseed extract. In addition, SHED treated with flaxseed extract resulted in low ALP activity compared to control (p<0.05) (t-test). Calcium deposition was positive in SHED cultured in OIM only. Conclusion: Flaxseed appears to reduce the expression of osteoblast markers, ALP activity and calcium deposition in SHED. This suggests that flaxseed as a potential inhibitor in osteoblast differentiation of SHED. 2019-10-19 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/75491/1/75491_ICPRP%202019%20%28Cover%29.pdf application/pdf en http://irep.iium.edu.my/75491/2/75491_ICPRP%202019%20%28Program%20Book%29.pdf application/pdf en http://irep.iium.edu.my/75491/3/75491_ICPRP%202019%20%28Poster%29.pdf Nordin, Nur Sazwi and Mokhtar@Makhtar, Khairani Idah and Mustafa Al-Ahmad, Basma Ezzat and Lestari, Widya and Arief Ichwan, Solachuddin Jauhari and Darnis, Deny Susanti and Thirumulu Ponnuraj, Kannan and Ahmad, Azlina (2019) Effects of flaxseed (Linum usitatissimum) extract on osteoblast differentiation potential of stem cells derived from human exfoliated deciduous teeth (SHED). In: International Conference on Pharmaceutical Research and Pharmacy Practice cum 14th IIUM-MPS Pharmacy Scientific Conference (ICPRP 2019), 19-20 October 2019, Istana Hotel, Kuala lumpur. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English
topic	RK Dentistry
spellingShingle	RK Dentistry Nordin, Nur Sazwi Mokhtar@Makhtar, Khairani Idah Mustafa Al-Ahmad, Basma Ezzat Lestari, Widya Arief Ichwan, Solachuddin Jauhari Darnis, Deny Susanti Thirumulu Ponnuraj, Kannan Ahmad, Azlina Effects of flaxseed (Linum usitatissimum) extract on osteoblast differentiation potential of stem cells derived from human exfoliated deciduous teeth (SHED)
description	Introduction: Flaxseed extract promotes bone health and possibly function in inducing bone regeneration process. SHED have been considered as an alternative source of MSC in tissue engineering because of their potential to differentiate into multiple cell-lineages including osteoblasts. Although flaxseed extract has been shown to affect bone formation process, its effect in inducing stem cell into osteoblast lineage has not been reported. Objective: To determine the effects of flaxseed extract on osteoblast differentiation potential of SHED. Materials and methods: SHED cultured in osteoblast induction media (OIM) was treated with flaxseed extract at 4mg/ml. The RNA was collected and extracted with Total RNA Mini Kit (Geneaid) from cells cultured at day 1, 3, 7, 14 and 21 and subjected to reverse-transcriptase PCR for osteoblast markers (OSX, OCN, DMP1). Alkaline phosphatase activity was determined by ALP assay and Alizarin Red S staining was performed to evaluate calcium deposits in SHED. Results: All osteoblast markers were expressed in all samples analysed. Interestingly, the expression of OSX appeared to be reduced in SHED treated with flaxseed extract. In addition, SHED treated with flaxseed extract resulted in low ALP activity compared to control (p<0.05) (t-test). Calcium deposition was positive in SHED cultured in OIM only. Conclusion: Flaxseed appears to reduce the expression of osteoblast markers, ALP activity and calcium deposition in SHED. This suggests that flaxseed as a potential inhibitor in osteoblast differentiation of SHED.
format	Conference or Workshop Item
author	Nordin, Nur Sazwi Mokhtar@Makhtar, Khairani Idah Mustafa Al-Ahmad, Basma Ezzat Lestari, Widya Arief Ichwan, Solachuddin Jauhari Darnis, Deny Susanti Thirumulu Ponnuraj, Kannan Ahmad, Azlina
author_facet	Nordin, Nur Sazwi Mokhtar@Makhtar, Khairani Idah Mustafa Al-Ahmad, Basma Ezzat Lestari, Widya Arief Ichwan, Solachuddin Jauhari Darnis, Deny Susanti Thirumulu Ponnuraj, Kannan Ahmad, Azlina
author_sort	Nordin, Nur Sazwi
title	Effects of flaxseed (Linum usitatissimum) extract on osteoblast differentiation potential of stem cells derived from human exfoliated deciduous teeth (SHED)
title_short	Effects of flaxseed (Linum usitatissimum) extract on osteoblast differentiation potential of stem cells derived from human exfoliated deciduous teeth (SHED)
title_full	Effects of flaxseed (Linum usitatissimum) extract on osteoblast differentiation potential of stem cells derived from human exfoliated deciduous teeth (SHED)
title_fullStr	Effects of flaxseed (Linum usitatissimum) extract on osteoblast differentiation potential of stem cells derived from human exfoliated deciduous teeth (SHED)
title_full_unstemmed	Effects of flaxseed (Linum usitatissimum) extract on osteoblast differentiation potential of stem cells derived from human exfoliated deciduous teeth (SHED)
title_sort	effects of flaxseed (linum usitatissimum) extract on osteoblast differentiation potential of stem cells derived from human exfoliated deciduous teeth (shed)
publishDate	2019
url	http://irep.iium.edu.my/75491/ http://irep.iium.edu.my/75491/1/75491_ICPRP%202019%20%28Cover%29.pdf http://irep.iium.edu.my/75491/2/75491_ICPRP%202019%20%28Program%20Book%29.pdf http://irep.iium.edu.my/75491/3/75491_ICPRP%202019%20%28Poster%29.pdf
first_indexed	2023-09-18T21:46:49Z
last_indexed	2023-09-18T21:46:49Z
_version_	1777413505341718528

Effects of flaxseed (Linum usitatissimum) extract on osteoblast differentiation potential of stem cells derived from human exfoliated deciduous teeth (SHED)

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