Salasa adulis as a novel dual-functional food additive for antimicrobial and antioxidant properties: experimental and theoretical approach

Lipid oxidation and microbial contamination are the major contributors to quality and safety loss of food products. The addition of food additives such as antioxidant and antimicrobial can increase the shelf life of foods and influence the characteristics of foods favourably. Salasa adulis or salak...

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Main Authors:	Ahmad, Mohammad Norazmi, Abdullah, Erna Normaya, Abu Samah, Mohd Armi, Omar, Muhammad Nor
Format:	Conference or Workshop Item
Language:	English English
Published:	Universiti Kuala Lumpur Branch Campus Malaysian Institute of Chemical and Bioengineering Technology 2019
Subjects:	QD Chemistry
Online Access:	http://irep.iium.edu.my/75324/ http://irep.iium.edu.my/75324/7/75324%20SALASA%20ADULIS%20AS%20A%20NOVEL.pdf http://irep.iium.edu.my/75324/13/SKAM32%20Presentation%20Ahmad%20et%20al.%202019b.pdf

id	iium-75324
recordtype	eprints
spelling	iium-753242019-11-15T05:43:49Z http://irep.iium.edu.my/75324/ Salasa adulis as a novel dual-functional food additive for antimicrobial and antioxidant properties: experimental and theoretical approach Ahmad, Mohammad Norazmi Abdullah, Erna Normaya Abu Samah, Mohd Armi Omar, Muhammad Nor Ahmad, Mohammad Norazmi QD Chemistry Lipid oxidation and microbial contamination are the major contributors to quality and safety loss of food products. The addition of food additives such as antioxidant and antimicrobial can increase the shelf life of foods and influence the characteristics of foods favourably. Salasa adulis or salak seeds contain phenolic compounds that exhibited interesting biological properties which contribute to its use as a new potential source of natural antioxidant and antimicrobial agent. This research aim to identify a novel phenolic compound of S. adulis seeds as a new antioxidant and antimicrobial inhibitor. A method development using green chemistry approach, supercritical fluid extraction (SFE) was used to extract the phenolic compounds from S. adulis seeds. The SFE conditions were optimized using Response Surface Methodology (RSM) that investigated the interaction between three variables (pressure, temperature, and extraction time). The optimum condition was achieved at 5814.67 psi, 78.09 ℃, and 96.43 min. The antioxidant activity test was done using DPPH radical scavenging assay where the % DPPH radical scavenging activity was 89.39 ± 0.16 %. The combination of GC-MS and PCA method revealed the potentiality of benzeneethanamine, 2,5- difluoro-β,3,4-trihydroxy-N-methyl- (C5) as antimicrobial agent. The suitability of carbon dioxide as the solvent for supercritical fluid extraction to extract C5 was shown by COSMO RS. MEP was used to identify key structural features of C5 that are necessary for their activity and to investigate their interaction with β ketoacyl-acyl carrier protein synthase III (FabH). The insight of microbial inhibitory action by C5 was carried out by using molecular docking and revealed the binding affinity with E. coli FabH-CoA was -6.1 kcal/mol. Universiti Kuala Lumpur Branch Campus Malaysian Institute of Chemical and Bioengineering Technology 2019 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/75324/7/75324%20SALASA%20ADULIS%20AS%20A%20NOVEL.pdf application/pdf en http://irep.iium.edu.my/75324/13/SKAM32%20Presentation%20Ahmad%20et%20al.%202019b.pdf Ahmad, Mohammad Norazmi and Abdullah, Erna Normaya and Abu Samah, Mohd Armi and Omar, Muhammad Nor and Ahmad, Mohammad Norazmi (2019) Salasa adulis as a novel dual-functional food additive for antimicrobial and antioxidant properties: experimental and theoretical approach. In: The 32nd Symposium of Malaysia Analytical Sciences : Simposium KImia Analisis Malaysia (SKAM 32), 14th-16th August 2019, Port Dickson, Negeri Sembilan.
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	QD Chemistry
spellingShingle	QD Chemistry Ahmad, Mohammad Norazmi Abdullah, Erna Normaya Abu Samah, Mohd Armi Omar, Muhammad Nor Ahmad, Mohammad Norazmi Salasa adulis as a novel dual-functional food additive for antimicrobial and antioxidant properties: experimental and theoretical approach
description	Lipid oxidation and microbial contamination are the major contributors to quality and safety loss of food products. The addition of food additives such as antioxidant and antimicrobial can increase the shelf life of foods and influence the characteristics of foods favourably. Salasa adulis or salak seeds contain phenolic compounds that exhibited interesting biological properties which contribute to its use as a new potential source of natural antioxidant and antimicrobial agent. This research aim to identify a novel phenolic compound of S. adulis seeds as a new antioxidant and antimicrobial inhibitor. A method development using green chemistry approach, supercritical fluid extraction (SFE) was used to extract the phenolic compounds from S. adulis seeds. The SFE conditions were optimized using Response Surface Methodology (RSM) that investigated the interaction between three variables (pressure, temperature, and extraction time). The optimum condition was achieved at 5814.67 psi, 78.09 ℃, and 96.43 min. The antioxidant activity test was done using DPPH radical scavenging assay where the % DPPH radical scavenging activity was 89.39 ± 0.16 %. The combination of GC-MS and PCA method revealed the potentiality of benzeneethanamine, 2,5- difluoro-β,3,4-trihydroxy-N-methyl- (C5) as antimicrobial agent. The suitability of carbon dioxide as the solvent for supercritical fluid extraction to extract C5 was shown by COSMO RS. MEP was used to identify key structural features of C5 that are necessary for their activity and to investigate their interaction with β ketoacyl-acyl carrier protein synthase III (FabH). The insight of microbial inhibitory action by C5 was carried out by using molecular docking and revealed the binding affinity with E. coli FabH-CoA was -6.1 kcal/mol.
format	Conference or Workshop Item
author	Ahmad, Mohammad Norazmi Abdullah, Erna Normaya Abu Samah, Mohd Armi Omar, Muhammad Nor Ahmad, Mohammad Norazmi
author_facet	Ahmad, Mohammad Norazmi Abdullah, Erna Normaya Abu Samah, Mohd Armi Omar, Muhammad Nor Ahmad, Mohammad Norazmi
author_sort	Ahmad, Mohammad Norazmi
title	Salasa adulis as a novel dual-functional food additive for antimicrobial and antioxidant properties: experimental and theoretical approach
title_short	Salasa adulis as a novel dual-functional food additive for antimicrobial and antioxidant properties: experimental and theoretical approach
title_full	Salasa adulis as a novel dual-functional food additive for antimicrobial and antioxidant properties: experimental and theoretical approach
title_fullStr	Salasa adulis as a novel dual-functional food additive for antimicrobial and antioxidant properties: experimental and theoretical approach
title_full_unstemmed	Salasa adulis as a novel dual-functional food additive for antimicrobial and antioxidant properties: experimental and theoretical approach
title_sort	salasa adulis as a novel dual-functional food additive for antimicrobial and antioxidant properties: experimental and theoretical approach
publisher	Universiti Kuala Lumpur Branch Campus Malaysian Institute of Chemical and Bioengineering Technology
publishDate	2019
url	http://irep.iium.edu.my/75324/ http://irep.iium.edu.my/75324/7/75324%20SALASA%20ADULIS%20AS%20A%20NOVEL.pdf http://irep.iium.edu.my/75324/13/SKAM32%20Presentation%20Ahmad%20et%20al.%202019b.pdf
first_indexed	2023-09-18T21:46:35Z
last_indexed	2023-09-18T21:46:35Z
_version_	1777413490458230784

Salasa adulis as a novel dual-functional food additive for antimicrobial and antioxidant properties: experimental and theoretical approach

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