Factors regulating nitric oxide production in spontaneously hypertensive rats treated with piper sarmentosum aqueous extract

Introduction: High blood pressure is a major risk factor for cardiovascular diseases which is one of the leading causes of death worldwide. Piper sarmentosum (PS) leaves have been widely used in traditional medicine and it has been proven for its antihypertensive and antioxidant effects. This stu...

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Main Authors:	Mohd Zainudin, Maizura, FT Elshami, Taher, Ismawi, Hidayatul Radziah, Hashim Fauzy, Fatimatuzzahra
Format:	Article
Language:	English English
Published:	Kulliyyah (Faculty) of Medicine, International Islamic University Malaysia 2019
Subjects:	R Medicine (General)
Online Access:	http://irep.iium.edu.my/75232/ http://irep.iium.edu.my/75232/ http://irep.iium.edu.my/75232/13/75232%20%20Factors%20Regulating%20Nitric%20Oxide.pdf http://irep.iium.edu.my/75232/19/75232_Factors%20Regulating%20Nitric%20Oxide%20Production_WoS.pdf

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spelling	iium-752322020-01-30T07:11:34Z http://irep.iium.edu.my/75232/ Factors regulating nitric oxide production in spontaneously hypertensive rats treated with piper sarmentosum aqueous extract Mohd Zainudin, Maizura FT Elshami, Taher Ismawi, Hidayatul Radziah Hashim Fauzy, Fatimatuzzahra R Medicine (General) Introduction: High blood pressure is a major risk factor for cardiovascular diseases which is one of the leading causes of death worldwide. Piper sarmentosum (PS) leaves have been widely used in traditional medicine and it has been proven for its antihypertensive and antioxidant effects. This study aims to evaluate the antihypertensive potential of PS aqueous extract (PSAE) and to investigate the factors modulating nitric oxide (NO) production through its anti-oxidant activities. Materials and Methods: PS leaves were extracted with distilled water and freezedried. The PSAE was examined to quantify their antioxidant activities through DPPH and FRAP test. It is also screened for total phenolic and flavonoids content. The antihypertensive effect of PSAE in SHR was evaluated using four different groups (n=6); C group (negative control), K group (PSAE 500mg/kg), P group (3 mg/kg perindopril) and M group (PSAE + 1.5 mg/kg perindopril). PSAE and other treatment were given via oral gavage for 28 consecutive days. The blood pressure and heart rate were determined using the non-invasive blood pressure monitoring (NIBPM) tail cuff technique and recorded weekly for four weeks. SHR’s blood was collected for the determination of serum NO level using Griess assay. Asymmetric dimethylarginine (ADMA) and arginine levels were determined using HPLC. Results: PSAE shows good in-vitro antioxidant activities with significant reduction of BP and decreased serum ADMA level. Conclusion: PSAE enhances the clearance of ADMA that maintains arginine level, thus increasing the serum NO level, which ameliorate the blood pressure of SHR. Kulliyyah (Faculty) of Medicine, International Islamic University Malaysia 2019 Article PeerReviewed application/pdf en http://irep.iium.edu.my/75232/13/75232%20%20Factors%20Regulating%20Nitric%20Oxide.pdf application/pdf en http://irep.iium.edu.my/75232/19/75232_Factors%20Regulating%20Nitric%20Oxide%20Production_WoS.pdf Mohd Zainudin, Maizura and FT Elshami, Taher and Ismawi, Hidayatul Radziah and Hashim Fauzy, Fatimatuzzahra (2019) Factors regulating nitric oxide production in spontaneously hypertensive rats treated with piper sarmentosum aqueous extract. International Medical Journal of Malaysia, 17 (Supplementary Issue No 1). p. 19. ISSN 1823-4631 E-ISSN 1823-4631 http://iiumedic.net/imjm/v1/download/vol_17_no_3_supp_1/MRS2018-20.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	R Medicine (General)
spellingShingle	R Medicine (General) Mohd Zainudin, Maizura FT Elshami, Taher Ismawi, Hidayatul Radziah Hashim Fauzy, Fatimatuzzahra Factors regulating nitric oxide production in spontaneously hypertensive rats treated with piper sarmentosum aqueous extract
description	Introduction: High blood pressure is a major risk factor for cardiovascular diseases which is one of the leading causes of death worldwide. Piper sarmentosum (PS) leaves have been widely used in traditional medicine and it has been proven for its antihypertensive and antioxidant effects. This study aims to evaluate the antihypertensive potential of PS aqueous extract (PSAE) and to investigate the factors modulating nitric oxide (NO) production through its anti-oxidant activities. Materials and Methods: PS leaves were extracted with distilled water and freezedried. The PSAE was examined to quantify their antioxidant activities through DPPH and FRAP test. It is also screened for total phenolic and flavonoids content. The antihypertensive effect of PSAE in SHR was evaluated using four different groups (n=6); C group (negative control), K group (PSAE 500mg/kg), P group (3 mg/kg perindopril) and M group (PSAE + 1.5 mg/kg perindopril). PSAE and other treatment were given via oral gavage for 28 consecutive days. The blood pressure and heart rate were determined using the non-invasive blood pressure monitoring (NIBPM) tail cuff technique and recorded weekly for four weeks. SHR’s blood was collected for the determination of serum NO level using Griess assay. Asymmetric dimethylarginine (ADMA) and arginine levels were determined using HPLC. Results: PSAE shows good in-vitro antioxidant activities with significant reduction of BP and decreased serum ADMA level. Conclusion: PSAE enhances the clearance of ADMA that maintains arginine level, thus increasing the serum NO level, which ameliorate the blood pressure of SHR.
format	Article
author	Mohd Zainudin, Maizura FT Elshami, Taher Ismawi, Hidayatul Radziah Hashim Fauzy, Fatimatuzzahra
author_facet	Mohd Zainudin, Maizura FT Elshami, Taher Ismawi, Hidayatul Radziah Hashim Fauzy, Fatimatuzzahra
author_sort	Mohd Zainudin, Maizura
title	Factors regulating nitric oxide production in spontaneously hypertensive rats treated with piper sarmentosum aqueous extract
title_short	Factors regulating nitric oxide production in spontaneously hypertensive rats treated with piper sarmentosum aqueous extract
title_full	Factors regulating nitric oxide production in spontaneously hypertensive rats treated with piper sarmentosum aqueous extract
title_fullStr	Factors regulating nitric oxide production in spontaneously hypertensive rats treated with piper sarmentosum aqueous extract
title_full_unstemmed	Factors regulating nitric oxide production in spontaneously hypertensive rats treated with piper sarmentosum aqueous extract
title_sort	factors regulating nitric oxide production in spontaneously hypertensive rats treated with piper sarmentosum aqueous extract
publisher	Kulliyyah (Faculty) of Medicine, International Islamic University Malaysia
publishDate	2019
url	http://irep.iium.edu.my/75232/ http://irep.iium.edu.my/75232/ http://irep.iium.edu.my/75232/13/75232%20%20Factors%20Regulating%20Nitric%20Oxide.pdf http://irep.iium.edu.my/75232/19/75232_Factors%20Regulating%20Nitric%20Oxide%20Production_WoS.pdf
first_indexed	2023-09-18T21:46:27Z
last_indexed	2023-09-18T21:46:27Z
_version_	1777413481889267712

Factors regulating nitric oxide production in spontaneously hypertensive rats treated with piper sarmentosum aqueous extract

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