Factors regulating nitric oxide production in spontaneously hypertensive rats treated with piper sarmentosum aqueous extract
Introduction: High blood pressure is a major risk factor for cardiovascular diseases which is one of the leading causes of death worldwide. Piper sarmentosum (PS) leaves have been widely used in traditional medicine and it has been proven for its antihypertensive and antioxidant effects. This stu...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English English |
| Published: |
Kulliyyah (Faculty) of Medicine, International Islamic University Malaysia
2019
|
| Subjects: | |
| Online Access: | http://irep.iium.edu.my/75232/ http://irep.iium.edu.my/75232/ http://irep.iium.edu.my/75232/13/75232%20%20Factors%20Regulating%20Nitric%20Oxide.pdf http://irep.iium.edu.my/75232/19/75232_Factors%20Regulating%20Nitric%20Oxide%20Production_WoS.pdf |
| Summary: | Introduction: High blood pressure is a major risk factor for cardiovascular diseases
which is one of the leading causes of death worldwide. Piper sarmentosum (PS)
leaves have been widely used in traditional medicine and it has been proven for its
antihypertensive and antioxidant effects. This study aims to evaluate the
antihypertensive potential of PS aqueous extract (PSAE) and to investigate the
factors modulating nitric oxide (NO) production through its anti-oxidant activities.
Materials and Methods: PS leaves were extracted with distilled water and freezedried. The PSAE was examined to quantify their antioxidant activities through
DPPH and FRAP test. It is also screened for total phenolic and flavonoids content.
The antihypertensive effect of PSAE in SHR was evaluated using four different
groups (n=6); C group (negative control), K group (PSAE 500mg/kg), P group (3
mg/kg perindopril) and M group (PSAE + 1.5 mg/kg perindopril). PSAE and other
treatment were given via oral gavage for 28 consecutive days. The blood pressure
and heart rate were determined using the non-invasive blood pressure monitoring
(NIBPM) tail cuff technique and recorded weekly for four weeks. SHR’s blood was
collected for the determination of serum NO level using Griess assay. Asymmetric
dimethylarginine (ADMA) and arginine levels were determined using HPLC. Results:
PSAE shows good in-vitro antioxidant activities with significant reduction of BP and
decreased serum ADMA level. Conclusion: PSAE enhances the clearance of ADMA
that maintains arginine level, thus increasing the serum NO level, which
ameliorate the blood pressure of SHR. |
|---|